Objective:To evaluate the diagnostic value of different autoantibodies combined detection in the Systemic Lupus Er -ythematosus ( SLE) .Methods:Collected 68 patients of SLE and 56 cases of other autoimmune disease ( AID) and the 50 patients of the control group .The dsDNA was tested by indirect immunofluorescence ( IIF ) and the Quantitative enzyme linked immunosorbent assay(ELISA), and the ANA was tested by IIF too, and the Linear Western blot detected 15 species-specific IgG class antibodies (ANA spectrum 3).Screened better indicators with SLE , and compared to the positive rate of different autoantibodies detection mode of SLE.Results:The positive rate was significantly different in SLE group and other AID group compared with the normal control group (P<0.01).Screened six better indicators with SLE:ANA, dsDNA, Sm ,Nucleosome ,Histone, ARPA (anti-ribosomal P protein an-tibodies).The positive rate of ANA, dsDNA, Nucleosome combined was significantly higher than the other three autoantibodies detection mode (ANA, dsDNA, Sm mode and ANA, dsDNA,Histone mode and ANA, dsDNA, ARPA mode) (P<0.01). Conclusion:They have important diagnostic value for SLE of ANA , dsDNA, Sm, Nucleosome, Histone, ARPA.The combined detection mode of the different autoantibodies can significantly improve the detection rate of SLE .Combined detection of ANA , dsDNA, Nucleosome have the diagnostic value to SLE .