1.Biochemical investigation and biological evaluation of the methanolic extract of the leaves of Nyctanthes arbortristis in vitro
Saha Kumer Repon ; Acharya Srijan ; Apu Sarker Apurba ; Roy Priyanka
Asian Pacific Journal of Tropical Biomedicine 2012;(z3):1534-1541
Objective: Nyctanthes arbortristis is a common plant in Bangladesh. The objective of our research was to biochemical and biological analysis of the methanolic extract of the dried leaves of Nyctanthesarbortristis found in Bangladesh. Methods: We investigated the presence of polyphenols, flavanoids and other types of compounds by thin layer chromatography, infrared spectroscopy, and UV spectroscopy analysis. We performed antioxidant assay by colorimetric methods. We investigated antibacterial assay by disk diffusion method. Cell surface receptor binding assay was performed by hemagglutination inhibition assay and hemolysis assay. Results: Methanolic extract of the leaves of Nyctanthes arbortristis contains flavanoids and other biologically active compounds. The extract showed antioxidant, peroxide scavenging and total reducing activity. The extract also showed antibacterial activities against several strains of bacteria. It also showed hemaglutination inhibition activities and hydrogen peroxide induced hemolysis inhibition activity in human blood cells. Conclusions: Therefore, Nyctanthes arbortristis may be considered as a plant of various health benefits.
2.HPLC analysis and cell surface receptor binding activities of the crude aqueous and methanolic extract of Sesamum indicum
Saha Kumer Repon ; Nabila Akther Kausain ; Roy Priyanka
Asian Pacific Journal of Tropical Biomedicine 2014;(z1):516-520
Objective: To identify the possible functional molecules for therapeutic uses by screening the crude aqueous and methanolic extracts derived from sesame seeds (Sesamum indicum) in vitro. Methods: High performance liquid chromatography was used to scan the functional molecules present in the extracts.Results:or its derivatives like molecules. On the other hand, the crude methanolic extract may contain Loratadine or its derivatives like molecules. Both type of extracts showed hemagglutination inhibition activities in all types of human blood samples tested. However, they showed stronger binding with AB+ blood group than those of A+ and B+ blood. The crude aqueous extracts showed the possibilities to present caffeine and cetirizine Conclusions: Sesame seeds may be considered as a functional food.
3.HPLC analysis and cell surface receptor binding activities of the crude aqueous and methanolic extract of Sesamum indicum.
Repon Kumer SAHA ; Md Abu Monsur DINAR ; Kausain Akther NABILA ; Priyanka ROY
Asian Pacific Journal of Tropical Biomedicine 2014;4(Suppl 1):S516-20
OBJECTIVETo identify the possible functional molecules for therapeutic uses by screening the crude aqueous and methanolic extracts derived from sesame seeds (Sesamum indicum) in vitro.
METHODSHigh performance liquid chromatography was used to scan the functional molecules present in the extracts.
RESULTSThe crude aqueous extracts showed the possibilities to present caffeine and cetirizine or its derivatives like molecules. On the other hand, the crude methanolic extract may contain Loratadine or its derivatives like molecules. Both type of extracts showed hemagglutination inhibition activities in all types of human blood samples tested. However, they showed stronger binding with AB+ blood group than those of A+ and B+ blood.
CONCLUSIONSSesame seeds may be considered as a functional food.