Objectives: This study aimed to evaluate the content, quality and demographics of YouTube videos about rubber dam as an information source for clinicians and dental students. Materials and Methods: “Rubber dam,” “rubber dam application,” “dental isolation,” “rubber dam isolation,” and “dental dam” were determined as keywords for the detection of YouTube videos related to rubber dam. Seventy 3 videos were evaluated and a total of 34 videos met the inclusion criteria. All selected videos were evaluated according to 8 parameters. The videos were scored 1 if the videos contained information about the selected parameter, but if the videos did not contain enough information, they were scored 0. The data were statistically analyzed with the analysis of variance and post hoc Tukey test (p < 0.05). Results: We found that 41% of the videos have poor, 47% have moderate, and 12% have good information. There is a statistically significant difference in time between poor and good information content (p < 0.05). There is a statistically significant difference between the poor and good information in the video information and quality index 1. Conclusions Rubber dam-related videos available on YouTube are generally moderately informed and insufficient. YouTube is currently not sufficient as a source of information for patients and clinicians at the moment. The YouTube platform should be developed and enriched with quality information on current and dental issues.