1.Altered expression of MALAT1 lncRNA in chronic lymphocytic leukemia patients, correlation with cytogenetic findings.
Abdolrahim AHMADI ; Saeid KAVIANI ; Marjan YAGHMAIE ; Hossein PASHAIEFAR ; Mohammad AHMADVAND ; Mahdi JALILI ; Kamran ALIMOGHADDAM ; Mohammad ESLAMIJOUYBARI ; Ardeshir GHAVAMZADEH
Blood Research 2018;53(4):320-324
BACKGROUND: Recent studies have devoted much attention to non-protein-coding transcripts in relation to a wide range of malignancies. MALAT1, a long non-coding RNA, has been reported to be associated with cancer progression and prognosis. Thus, we here determined MALAT1 gene expression in chronic lymphocytic leukemia (CLL), a genetically heterogeneous disease, and explored its possible relationships with cytogenetic abnormalities. METHODS: MALAT1 expression level was evaluated using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) on blood mononuclear cells from 30 non-treated CLL patients and 30 matched healthy controls. Cytogenetic abnormalities were determined in patients by fluorescence in situ hybridization (FISH). RESULTS: MALAT1 expression level was up-regulated in the CLL group compared to healthy controls (P=0.008). Del13q14, followed by Del11q22, were the most prevalent cytogenetic abnormalities. We found no association between the FISH results and MALAT1 expression in patients. CONCLUSION: Altered expression of MALAT1 is associated with CLL development. Further investigations are required to assess the relationship between this long non-coding RNA and CLL patient survival and prognosis.
Chromosome Aberrations
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Cytogenetics*
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Fluorescence
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Gene Expression
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Humans
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In Situ Hybridization
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Leukemia, Lymphocytic, Chronic, B-Cell*
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Polymerase Chain Reaction
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Prognosis
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Reverse Transcription
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RNA, Long Noncoding*