This study was conducted to evaluate the effect of argan oil with the exposure of low frequency electromagnetic field (EMF)on open wound healing in mice. Eighteen male mice (20-40 g) were divided into three groups: phosphate buffer saline(PBS) as negative control, solcoseryl gel as positive control, and argan oil with the exposure of low frequency EMF, 1.2mT (treatment group). Full thickness wounds (4 mm diameter) were induced on the shaved dorsal of the mouse. All micewere sacrificed on day 12 after the final treatment. Macroscopic observation, wound contraction rate, histopathologyanalysis and total protein content were examined in this study. Results showed that wounds treated with argan oil andexposed to low frequency EMF has a significant increase in wound contraction rate (p < 0.05) and total protein content(p < 0.05). Moreover, histopathological analysis on the wound tissues displayed complete re-epithelization with thick anddense collagen fibers in the argan oil with low frequency EMF exposure treated group. In conclusion, topical treatment ofargan oil with low frequency EMF exposure yield a better healing progress and showed the ability to accelerate woundhealing.