Objective:The prokaryotic expression vector of human anti-Siglec-9 Fab fragment antibody was constructed and purified,while was identified. Methods:The variable and conserved regions of heavy chain and light chain were obtained by polymerase chain reaction respectively(PCR),which was combined by overlap extension PCR and was digested with restriction enzyme,and then it was transformed into Escherichia coli BL21 and was purified by His-trap Lambda Fab column and AKTA system. SDS-PAGE,ELISA and Western blot were used for the identification of human anti-Siglec-9 Fab fragment antibody. The effect of human anti-Siglec-9 Fab fragment antibody on regulating the mRNA expression of TNF-α,IL-1,IL-6,IL-8 was detected by real-time PCR. Results:Successfully obtained the chains of heavy and light, while constructed an activation human anti-Siglec-9 Fab fragment antibody which could specifically bind to Siglec-9 protein. The human anti-Siglec-9 Fab fragment antibody could specifically bind to Siglec-9 was confirmed by SDS-PAGE,ELISA and Western blot. The human anti-Siglec-9 Fab fragment antibody inhibited the mRNA expression of TNF-α,IL-1, IL-6,IL-8. Conclusion:Successful prokaryotic expression,purification,character analysis,and suppressed the mRNA expression of in-flammatory cytokines with the human anti-Siglec-9 Fab fragment antibody and lay the biology foundation for the further study.

No abstract available.
Meningitis*

No abstract available.
Heterotaxy Syndrome*

Objective To establish a method for quantitative determination of 4 chemical constituentsgeniposide,genipin-1-β-D-gentiobioside,arctiin and baicalin in the effective fraction of Zhibangreduping Formula.Methods The procedare of HPLC was performed on the chromatographic column of Diamonsil C_(18)(4.6 mm×150 mm,5μm),and the mobile phase in the gradient elution was acetonitrile-phosphoric acid solution(0.2%).The flow rate was 1.0 mL/min and the detection wavelength was 238 nm and 278 nm.Results The average recovery of geniposide WaS 99.02%(RSD=1.99%,n=6),genipin-1-β-D-gentiobioside,99.03%(RSD=2.83%,n=6),arctiin,101.12%(RSD=2.86%,n=6),and baicalin,100.05%(RsD=2.02%.n=6).Conclusion The results of determination in 3 batches of samples show that the method is easy and accurate,which can be used in the quantitative determination of 4 chemical constituents in the effective fraction of Zhibangreduping Formula.

Objective To establish the characteristic chromatograms of Mongolian compound Sengdeng-4 in vivo and in vitro, analyze and identify its chemical components in vitro and serum. Methods After the model of rheumatoid arthritis was established, SD rats were orally administered Mongolian compound Sengdeng-4 and each single ingredient respectively. The medicinal serum were separated and measured with HPLC. The HPLC characteristic chromatograms of Mongolian compound Sengdeng-4 in vitro and medicinal serum were identified. The characteristic chromatograms of Mongolian compound Sengdeng-4, single ingredient in vitro and medicinal serum were analyzed, and the chemical composition of Mongolian compound Sengdeng-4 in vitro, original crude medicinal in blood were identified and compared. Results The results of HPLC characteristic chromatograms of Mongolian compound Sengdeng-4 showed that 47 peaks were identified. There were 14 chemical compositions absorbed into blood, among them 9 were original ingredients and 5 were metabolites. The second peak was from Wenguanmu; the fifth, sixth, and seventh peaks were from Zhizi; the first, eighth, and tenth peaks were from Hezi. The third peak was from Wenguanmu, Chuanlianzi and Hezi. The fourth and thirteenth peaks were from Mongolian compound Sengdeng-4. The ninth peak was from Wenguanmu and Chuanlianzi. The eleventh, twelfth
and fourteenth peaks were from Wenguanmu and Hezi. 7 components of Mongolian compound Sengdeng-4 were identified. Conclusion The chemical compositions and metabolites in blood may be the active ingredients with direct actions in vivo, which come from the ingredients of Mongolian compound Sengdeng-4 .

PURPOSE: This study was to identify knowledge, perception and health behavior about metabolic syndrome for an at risk group in a rural community area. METHODS: A descriptive cross-sectional survey design was used. A total of 575 adults with hypertension, diabetes mellitus, dyslipidemia, and/or abdominal obesity were recruited from 11 rural community health care centers. A questionnaire was developed for this study. Anthropometric measures were measured and blood data was reviewed from the health record. RESULTS: Knowledge about the metabolic syndrome was low as evidenced by only a 47% correct answer rate. Only 9% of the subjects ever heard about the disease, and 87% answered they do not know the disease at all. 87% of the subjects were not performing regular exercise, 31% drank alcohol more than once a month, 12.5% were current smokers, and 33.6% are did not have a regular health check-up. CONCLUSION: Development of systematic public health care programs are needed to prevent future increases in cardiovascular complications and to decrease health care costs. These might include educational programs for the primary health care provider and an at risk group, a therapeutic lifestyle modification program, and a health screening program to identify potential groups.
Aged ; Anthropometry ; Awareness ; Blood Chemical Analysis ; Cross-Sectional Studies ; Female ; *Health Behavior ; Health Care Surveys ; Health Knowledge, Attitudes, Practice ; Humans ; Life Style ; Male ; Metabolic Syndrome X/etiology/*psychology ; Middle Aged ; *Perception ; Risk Factors ; *Rural Health ; Rural Health Services/organization & administration

BACKGROUND: Vascular endothelial growth factor (VEGF) is a multifunctional cytokine involved in angiogenesis as selective mitogen for endothelial cells as well as potent permeability factor. And interleukin-6 (IL-6) is also known to be a growth factor of myeloma cells. To determine the role of angiogenesis, VEGF and IL-6 in the patients with multiple myeloma, the relationship between the level of VEGF expression, microvessel count (MVC), IL-6 expression in the bone marrow specimen of multiple myeloma patients and stage, response, survival duration were evaluated in 18 patients with multiple myeloma who underwent bone marrow biopsy. METHODS: VEGF expression, MVC and IL-6 expression were assessed by immunohistochemical stain with polyclonal antibody to VEGF, factor VIII related antigen and IL-6 respectively. RESULTS: VEGF expression was higher in multiple myeloma than that of control (61.4+/-34.4% vs 19.0+/-25.9%, P<0.001), and MVC was also higher in multiple myeloma than that of control (11.7+/-6.1 vs 6.2+/-3.8, P=0.005). IL-6 was expressed in 66.7% of multiple myeloma but not in control (P<0.001). Between high VEGF expression group and low VEGF expression group, there were no significant differences in the stage, response or survival. There were no significant differences between hypervascular group and hypovascular group. Also IL-6 expression was not a prognostic indicator. After treatment, VEGF expression, MVC and IL- 6 expression were decreased in the responder, but these differences were not statistically significant (P=0.23, P=0.07, P=0.06), probably due to limited number of cases. CONCLUSION: VEGF, angiogenesis and IL-6 can play a role in the pathogenesis of multiple myeloma. But we cannot confirm the prognostic role of those parameters. Further study with more cases in longer duration as well as prospective study would be necessary for the establishment of relationship between VEGF expression, neovascularization, IL-6 expression and disease severity and prognosis of multiple myeloma.
Biopsy ; Bone Marrow ; Endothelial Cells ; Humans ; Interleukin-6* ; Microvessels ; Multiple Myeloma* ; Permeability ; Prognosis ; Vascular Endothelial Growth Factor A* ; von Willebrand Factor

Anemia, Dyserythropoietic, Congenital/complications* ; Cholelithiasis/complications* ; Hemochromatosis ; Humans