CD4 Antigens ; metabolism ; CD56 Antigen ; metabolism ; Dendritic Cells ; pathology ; Diagnosis, Differential ; Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor ; Hematologic Neoplasms ; drug therapy ; genetics ; metabolism ; pathology ; surgery ; Humans ; Immunohistochemistry ; Leukemia, Myeloid ; pathology ; Lymphoma, Extranodal NK-T-Cell ; pathology ; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma ; pathology ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ; pathology ; Skin Neoplasms ; drug therapy ; genetics ; metabolism ; pathology ; surgery

Animals ; Eosinophilia ; genetics ; pathology ; Gene Rearrangement ; Humans ; Lymphoma ; genetics ; pathology ; Myeloproliferative Disorders ; genetics ; pathology ; Receptor, Fibroblast Growth Factor, Type 1 ; genetics ; Receptor, Platelet-Derived Growth Factor alpha ; genetics ; Receptor, Platelet-Derived Growth Factor beta ; genetics

Objective To investigate the characteristics of pharmacoki-netics of pingyangmycin ion -exchanged microspheres in rabbits.Methods A total of 12 Japanese rabbits, half male and half female, were randomly divided into group A and group B.The rabbits in group A and group B were injected with PYM and PYM-MS via external carotid artery separately.The blood samples were obtained at 2, 5, 10, 20, 40, 60 ,90 and 120 minutes after injection and detected by HPLC method.The parameters of pharmacokinetics were analyzed.Results Compared with group A, it showed a slow decrease in terms of concentration-time curves of pingyangmycin in rabbits after administration of PYM-MS in group B;the Cmax [(11.62 ±2.66) vs (58.96 ±4.51) mg· L-1 ] and AUC [(21.06 ±31.33) vs (791.98 ±55.30) mg· L-1 · min] in group B were significantly lower ( P <0.01 ) , but the data of MRT0-60 min was longer in group B [ ( 22.29 ±1.52 ) vs ( 14.20 ±1.24 ) min ] , P <0.01.Conclusion PYM-MS showed definite property of delayed drug release, with a lower plasma concentration.

Adolescent ; Anti-Bacterial Agents ; therapeutic use ; Calcitonin ; blood ; Calcitonin Gene-Related Peptide ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Protein Precursors ; blood ; Respiratory Tract Infections ; blood ; drug therapy

Liver depression and spleen deficiency syndrome is a common syndrome type of ulcerative colitis (UC). Based on the theory of 'collaborative treatment of liver and spleen', TCM shows its effect and less adverse reactions in the treatment of UC with liver depression and spleen deficiency. The internal treatment of TCM based on syndrome differentiation and treatment by stages, as well as the external treatment of TCM based on TCM enema therapy, acupuncture therapy and acupoint application therapy, can relieve symptoms, improve relevant clinical indicators and improve the quality of life of patients. The mechanism studies show that the TCM with the effect of soothing the liver and activating the spleen plays a therapeutic role by regulating signal pathway conduction, regulating intestinal flora, and improving the level of inflammatory factors.

Objective To investigate the ability in diagnosing and reporting brucellosis of primary medical personnel in Suide County of Shaanxi Province,and provide scientific basis for making prevention and control strategies.Methods Convenience sampling method was adopted in October 2016.A questionnaire survey was conducted among the county hospitals,township hospitals,village clinics and individual clinics in Suide County to collect information on the ability in diagnosing and reporting brucellosis of primary medical personnel.The specific content includes the basic information,the awareness of brucellosis diagnosis and reporting capabilities,the situation of brucellosis training,and the views about the diagnosis of brucellosis.SPSS 18.0 software was used to carry out descriptive statistical analysis.Results A total of 77 primary healthcare workers were surveyed.Their average age was (38 ± 9) years old,57.1％ (44/77) workers were undergraduates,and 53.2％ (41/77) were working in county hospitals.9.1％ (7/77) were completely aware of the major infectious diseases,29.9％ (23/77) were fully aware of the transmission mute,28.6％ (22/77) and 66.2％ (51/77) known the high risk population and the epidemiological history of brucellosis.Twenty-six percent (20/77) of the medical staff were thoroughly aware of the main clinical manifestations,68.8％ (53/77) were clearly aware of the clinical stage of brucellosis;24.7％ (19/77) could understand the specificity of the laboratory tests of brucellosis;44.2％ (34/77) had a strong ability in reporting brucellosis.71.4％ (55/77) of the medical staff received the training about the knowledge of brucellosis;49.4％ (38/77) thought that the clinical manifestation was the most difficult to grasp in the criteria about diagnosis of brucellosis.Conclusions The ability of diagnosis and reporting of brucellosis is weak in primary healthcare workers in Suide County,the epidemiology and clinical knowledge of brucellosis are not comprehensive.It is suggested to strengthen the understanding of brucellosis among medical staff,especially in primary healthcare workers.We should carry out the training of disease epidemiology and clinical knowledge of brucellosis and standardize patient management.

Objective To learn the diagnosis and treatment of brucellosis patients in Suide County of Shaanxi Province in 2015,and to provide a scientific basis for making accurate prevention and control measures.Methods All the cases diagnosed as brucellosis in Suide County from January 1 to December 31 in 2015 and lived in this county were retrospectively investigated by case questionnaire survey,including basic information,medical procedures,and epidemiological contacts.Descriptive statistical analysis was performed using SPSS 18.0 software.Results In 2015,75 cases of brucellosis were diagnosed in Suide County,75 questionnaires were distributed,with 53 completed questionnaires returned.The average age of respondents was (49 ± 14) years old,of which 84.9％ (45/53) were male and 94.3％ (50/53) were farmers,except one case was actively monitored by Suide County Centre for Disease Control and Prevention then treated,the rest of the patients were treated after the onset of the disease.Of the 52 patients,one patient was diagnosed after one visit,accounting for 25.0％ (13/52);one patient was diagnosed after at least 4 visits,accounting for 3.8％ (2/52).The cumulative diagnosis rate at provincial-level hospitals was 1/3 and that at disease prevention and control institutions was 92.7％ (51/55),there were no confirmed cases in municipal hospitals,county hospitals,township hospitals,village clinics and individual clinics.For the first reason to see a doctor,65.4％ (34/52) of the patients were fever or accompanied by sweating,fatigue,arthralgia and waist and leg pain.The medians from onset to treatment between the first diagnosis,and 2,3,4 visits were 15,18,27,45 days,respectively;the median from onset to diagnosis was 21 days,ranging from 2 to 182 days.Totally 84.9％ (45/53) patients had a history of exposure to animals,of which 97.8％ (44/45) had contacted the sheep.Conclusions The cases in Suide County are mainly adult male farmers,and the diagnosis rate at hospitals below municipal level is low.It is recommended to strengthen the health intervention of high-risk groups and improve the level of diagnosis and treatment of primary medical staff.

Objective To investigate the effects and potential mechanisms of ultrasound-guided thoracic paravertebral block(TPVB)combined with general anesthesia in patients undergoing thoraco-scopic surgery.Methods A total of 122 patients undergoing thoracoscopic surgery were selected and randomly divided into control group and tudy group,with 61 patients in each group.The control group received general anesthesia,while the study group received ultrasound-guided TPVB combined with general anesthesia.Clinical data and perioperative indicators were recorded and compared between the two groups.Mean arterial pressure(MAP),heart rate(HR),Visual Analogue Scale(VAS)scores before surgery,24 hours and 72 hours after surgery,and inflammatory factors[interleukin-6(IL-6),interleukin-10(IL-10),tumor necrosis factor-α(TNF-α)],oxidative stress indicators[malondialde-hyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)]levels before surgery and 72 hours after surgery were compared between the two groups.Postoperative adverse reactions were recorded.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of key molecules mRNA in the Nrf2/Keap1/ARE pathway in the peripheral blood of pa-tients in the two groups.Results There were no statistically significant differences in operative time and intraoperative blood loss between the two groups(P＞0.05).The study group had shorter extu-bation time and indwelling time of drainage tube,less total drainage volume,and less total PCIA drug consumption within 36 hours after surgery and times of patient controlled intravenous analgesia(PCIA)effective compression compared to the control group(P＜0.05).At 24 and 72 hours after surgery,MAP,HR,and VAS scores were higher than preoperative levels in the control group,while VAS scores were higher than preoperative levels in the study group,but MAP,HR,and VAS scores were lower in the study group compared to the control group(P＜0.05).At 72 hours after surgery,serum levels of IL-6,IL-10,TNF-α,and MDA were higher than preoperative levels,while SOD and GSH-Px levels were lower than preoperative levels in both groups.Additionally,the study group had lower serum levels of IL-6,TNF-α,and MDA and higher levels of IL-10,SOD,and GSH-Px compared to the control group(P＜0.05).The total incidence of postoperative adverse re-actions was 4.92％(3/61)in the study group,which was lower than 21.31％(13/61)in the con-trol group(P＜0.05).At 72 hours after surgery,the expression levels of Nrf2 mRNA and ARE mRNA in the peripheral blood of patients in the study group were lower than those in the control group,while the expression level of Keap1 mRNA was higher(P＜0.05).Conclusion Compared with general anesthesia,ultrasound-guided TPVB combined with general anesthesia can significantly improve hemodynamic stability and analgesic effects in patients with early-stage lung cancer after thoracoscopic surgery,relieve inflammatory and oxidative stress responses,and decrease the inci-dence of postoperative adverse reactions.The molecular mechanism may be related to the inhibition of Nrf2/Keap1/ARE signaling pathway activation.

Objective To investigate the effects and potential mechanisms of ultrasound-guided thoracic paravertebral block(TPVB)combined with general anesthesia in patients undergoing thoraco-scopic surgery.Methods A total of 122 patients undergoing thoracoscopic surgery were selected and randomly divided into control group and tudy group,with 61 patients in each group.The control group received general anesthesia,while the study group received ultrasound-guided TPVB combined with general anesthesia.Clinical data and perioperative indicators were recorded and compared between the two groups.Mean arterial pressure(MAP),heart rate(HR),Visual Analogue Scale(VAS)scores before surgery,24 hours and 72 hours after surgery,and inflammatory factors[interleukin-6(IL-6),interleukin-10(IL-10),tumor necrosis factor-α(TNF-α)],oxidative stress indicators[malondialde-hyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)]levels before surgery and 72 hours after surgery were compared between the two groups.Postoperative adverse reactions were recorded.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of key molecules mRNA in the Nrf2/Keap1/ARE pathway in the peripheral blood of pa-tients in the two groups.Results There were no statistically significant differences in operative time and intraoperative blood loss between the two groups(P＞0.05).The study group had shorter extu-bation time and indwelling time of drainage tube,less total drainage volume,and less total PCIA drug consumption within 36 hours after surgery and times of patient controlled intravenous analgesia(PCIA)effective compression compared to the control group(P＜0.05).At 24 and 72 hours after surgery,MAP,HR,and VAS scores were higher than preoperative levels in the control group,while VAS scores were higher than preoperative levels in the study group,but MAP,HR,and VAS scores were lower in the study group compared to the control group(P＜0.05).At 72 hours after surgery,serum levels of IL-6,IL-10,TNF-α,and MDA were higher than preoperative levels,while SOD and GSH-Px levels were lower than preoperative levels in both groups.Additionally,the study group had lower serum levels of IL-6,TNF-α,and MDA and higher levels of IL-10,SOD,and GSH-Px compared to the control group(P＜0.05).The total incidence of postoperative adverse re-actions was 4.92％(3/61)in the study group,which was lower than 21.31％(13/61)in the con-trol group(P＜0.05).At 72 hours after surgery,the expression levels of Nrf2 mRNA and ARE mRNA in the peripheral blood of patients in the study group were lower than those in the control group,while the expression level of Keap1 mRNA was higher(P＜0.05).Conclusion Compared with general anesthesia,ultrasound-guided TPVB combined with general anesthesia can significantly improve hemodynamic stability and analgesic effects in patients with early-stage lung cancer after thoracoscopic surgery,relieve inflammatory and oxidative stress responses,and decrease the inci-dence of postoperative adverse reactions.The molecular mechanism may be related to the inhibition of Nrf2/Keap1/ARE signaling pathway activation.

Objective:To investigate the value of fetal heart quantification (fetal HQ) in assessing ventricular function of fetuses with small-for-gestational-age (SGA) and fetal growth restriction (FGR).Methods:A total of 152 singleton pregnancies with estimated fetal weight (EFW) or abdominal circumference less than the 10th percentile in the Third Affiliated Hospital of Zhengzhou University were prospectively selected from August 2022 to September 2023, where fetal EFW or abdominal circumference were in the 3rd to 9th percentile with normal Doppler findings were classified as the SGA group ( n=79), and the rest as the FGR group ( n=73). In the same period, 161 cases of normal single fetuses were matched as the control group.Based on the gestational week in which FGR occurred, the FGR group were categorized into the early-onset group (<32 weeks, n=46) and the late-onset group (≥32 weeks, n=27), and fetuses in the FGR group with absent end-diastolic velocity of the umbilical artery were defined as severe FGR ( n=11), and the rest as mild FGR ( n=62). Fetal ventricular fractional area change (FAC), global longitudinal strain (GLS), longitudinal fractional shortening (LFS) and 24-segment fractional shortening (FS) were obtained by fetal HQ. The cardiac systolic function between groups were compared, the correlations between each parameter and gestational week were analyzed, the inter-observer and intra-observer repeatability tests were performed. Results:Compared with the control group, ventricular FAC, LFS, and GLS were lower in the SGA and the FGR group, right ventricular FS of segments 9-24 were reduced in the SGA group, and left ventricular FS of segments 10-19, 21-24 and right ventricular FS of segments 18-24 were reduced in the FGR group, the differences were statistically significant (all P<0.05). Left ventricular GLS, LFS and right ventricular FAC, GLS, LFS, FS of segments 1-14 were lower in the severe FGR group than in the mild FGR group, the differences were statistically significant (all P<0.05). The values of left ventricular GLS and LFS were higher in the early-onset FGR group than in the late-onset FGR group, the differences were statistically significant (all P<0.05). The ROC showed that the ventricular systolic function parameters predicted adverse perinatal outcomes with an AUC>0.6 (all P<0.05). Left ventricular GLS and right ventricular partial-segments FS were no correlations with gestational week in the FGR group (-0.3< rs<0.3, all P<0.05). There was no correlation between the parameters and gestational week in the SGA group (all P>0.05). The inter-observer and intra-observer intraclass correlation coefficients (ICC) were >0.75, with good reproducibility. Conclusions:Fetal HQ can quantitatively assess the changes of ventricular function in SGA and FGR fetuses, and the ventricular overall, longitudinal and localized contractile function in SGA and FGR fetuses are reduced, and abnormal ventricular systolic function is associated with adverse perinatal outcomes.