1.Establishment and optimization of sequence-related amplified polymorphism amplification system for Carthamus tinctorius L.
Academic Journal of Second Military Medical University 1985;0(05):-
Objective:To study the factors influencing the amplification of sequence-related amplified polymorphism(SRAP) system in Carthamus tinctorius L.and to establish a stable SRAP reaction system,laying a foundation for molecular marker assistant breeding of Carthamus tinctorius L.Methods: Cetyltrimethylammonium bromide method was used to extract the genomic DNA of Carthamus tinctorius L..Twenty-seven tests with 3 factors at 3 levels were designed,the conditions including Taq polymerase concentrations(0.02,0.04,0.06 U/?l),dNTP concentrations(0.15,0.25,0.30 mmol/L)and Primer concentrations(0.15,0.30,0.45 ?mol/L);another 8 tests were designed based on the single factor of Mg~(2+) concentrations(0.5,1.0,1.5,2.0,2.5,3.0,3.5 and 4.0 mmol/L).Twenty ng DNA template was added into 25 ?l SRAP reaction system;the system was optimized and agarose electrophoresis was used for determination.Results: A SRAP reaction system for Carthamus tinctorius L.was established.In a 25 ?l reaction system,Taq polymerase was 0.02 U/?l,dNTP was 0.25 mmol/L,Primer was 0.30 ?mol/L,and Mg~(2+) was 3.0 mmol/L.Target bands increased after optimization,with good reproducibility,and the amplification results were satisfactory.Conclusion: The SRAP reaction system in this experiment is suitable for analysis of Carthamus tinctorius L.
2.Normal Vestibulo Ocular Reflex (VOR) gain Measured Using the Video Head Impulse Test (vHIT) in Healthy Young Adults
The International Medical Journal Malaysia 2019;18(3):88-94
Introduction: Many new objective tests to assess the function of specific structures of the vestibular
organ are currently adopted in vestibular clinics. One of the objective assessments include the video head
impulse test (vHIT) where gain & velocity responses of eye relative to the head movements are recorded
using an infrared camera. Methods: Thirty normal hearing subjects age between 18 to 25 years old
participated in this study. At least ten Lateral, Left Anterior Right Posterior (LARP), and Right Anterior Left
Posterior (RALP) responses were recorded for each participant by making small and rapid unpredictable head
movements. Results: The average velocity gain for Lateral responses at 40 ms, 60 ms and 80 ms were 1.05 ±
0.003, 1.03 ± 0.002 and 1.01 ± 0.003 respectively. The LARP average velocity regression were 1.01 ± 0.24 for
Left Anterior and 1.05 ± 0.25 for Right Posterior, with an average gain asymmetry of 5.13%. The RALP
average velocity regression were 1.08 ± 0.31 for Right Anterior and 1.12 ± 0.30 for Left Posterior, with an
average gain asymmetry of 5.87%. One sample T-test were conducted to compare Lateral responses to a
previous study by Mossman et al. (2015) where significant differences in velocity gain at 60 ms and 80 ms
between studies were found where, t (59) = 5.56, p <0.01 and t (59) = 2.86, p < 0.01 respectively.
Conclusion: This indicates the importance of establishing on-site norms for every clinical settings as
techniques used and equipment differences could affect the results.
3.Hypoglyceamic effects of aqueous extract of Aframomum Melegueta leaf on alloxan-induced diabetic male albino rats.
Mojekwu TO ; Yama OE ; Ojokuku SA ; Oyebadejo SA.
Pacific Journal of Medical Sciences 2011;8(1):28-36
Aframomum melegueta (Zingiberaceae) seeds are used in West Africa, as a remedy for variety of ailments such as stomach ache, snakebite, diarrhea and anti-inflammatory properties. The hypoglycaemic effects of crude leaf extract of Aframomum melegueta on the treatment of alloxan
induced diabetes in male rats and non-diabetic rats (control) were examined in this study. Results
obtained from the experiment showed that the elevated blood glucose level caused by oral administration of 250 mg / kg body weight of alloxan was reduced significantly (p < 0.01) by oral administration of Aframomum melegueta leaf extract doses of 50, 100 and 200 mg/kg with the
exception of 20 mg/kg when compared to control groups. The non-diabetic groups that received the extract showed reduction in blood sugar level as the dose increases when compared to their control group. There was a final weight gain and organ restoration for both the diabetic and non-diabetic rats after treatment when compared with their controls. This study showed that the extract have
hypoglycemic and prophylactic effects.
4.Classification of Epileptic Seizures and Syndromes: Historical Review and Changes in Terms and Concepts.
Journal of the Korean Child Neurology Society 2001;9(1):178-191
No abstract available.
Classification*
;
Epilepsy*
5.Evaluation on environment sanitation in Ha Nam General Hospital
Journal of Medical and Pharmaceutical Information 2004;0(2):22-25
This study was carried out to evaluate environmental factors at Ha Nam General Hospital. The result showed that: about 640 kg of solid waste is generated everyday of which 22% is toxic. The equipments and infrastructure are under ability for treating this waste. Almost of the microclimatic parameters are in the normal value, but the illumination is a little low. The physical and chemical parameters in supplied water at the hospital are within the permitted standard. The hospital need to equip tools and equipment for collecting, transportation and treatment this waste according to the standards of Viet Nam Ministry of Health, standard waste bins
Environment, Sanitation
6.Environmental sanitation in Bac Ninh General Hospital in 2004
Journal of Medical and Pharmaceutical Information 2003;0(3):28-31
A study of environmental sanitation in Bac Ninh General Hospital was carried out in 2004 with certain objects: 145 patients and their relatives, 37 people living near the hospital, 35 health workers, and 5 managers of the hospital… The hospital delivered about 1570 kg of waste daily; 340 kg of them was dangerous and must be treated in special way. The hospital implemented medical solid waste management and treatment well; the incinerator had worked effectively in making the hospital’s environment fresh. The medical solid waste treatment system of this hospital was a good sample
Sanitation, Environment
7.Research Advance in the Role of Toll-like Receptors in Hepatocarcinogenesis
Journal of Kunming Medical University 2016;37(7):121-125
Hepatocellular carcinoma (HCC) is the second largest cause of cancer deaths worldwide.TLRs signal transduction pathways are related to the progress of HCC.As an innate immune recognition receptors on the surface of the cell,TLRs are involved in the ligand-mediated activation of various cells and the secretion of cytokines in liver through signal transduction,which is part of the cause and development of HCC.A better understanding of TLRs and these signaling pathways in the liver will help us understand the mechanism of hepatocarcinogenesis and provide a new therapeutic target for HCC.
8.Application of retroauricular fascial flap to postburn auricular reconstruction
Journal of Third Military Medical University 2002;0(12):-
Objective To introduce a new technique of one-stage auricular reconstruction by using the retroauricular fascial flap. Methods The retroauricular fascial flap was designed to cover a cartilage framework for auricular reconstruction. Results Seventeen cases who had injured over two years and received no surgical treatment, underwent auricular construction. In 3-month to 2-year follow-up, all patients obtained satisfactory appearance of the reconstructed ears that possess delicate structure and close color to adjacent skin. Conclusion For retroauricular fascial flap has adequate blood supply, it makes operating procedure simple and the occurrence rate of complication reduced. It is proven that this is a good method of auricular reconstruction.
9.DIFFERENTIATION OF MESENCHYMAL STEM CELLS INTO NEURON- LIKE CELLS WITH LIGUSTRAZIN HYDROCHLORIDE
Acta Anatomica Sinica 2002;0(05):-
Objective To investigate the differentiation of mesenchymal stem cells(rMSCs) of young rat into neuron-like cells with Ligustrazin hydrochloride. Methods rMSCs were separated from femurs marrow flushed out with DMEM(low glucose) by using a needle and syringe, then planted in plastic culture flask. Through expanded to 5 passages, rMSCs were induced to differentiate into neuron-like cells with Ligustrazin hydrochloride. Anti-neurofilament(NF-M), nestin, neuron-specific enolase(NSE), MAP-2,GAP-43 and glial fibrillary acidic protein(GFAP) antibodies were detected by immunohistochemistry. Results rMSCs were comprised a single phenotypic population and displayed a fibroblast-like morphology after 5 passage in culture. With 10*!?g/L bFGF pre-induce for 24*!h, then the medium was replaced with induction medium containing Ligustrazin hydrochloride. The induced-rMSCs exhibited neuronal morphological characteristics from the first half an hour to 5*!h. The neuron-like cells expressed NF-M, NSE, MAP-2,GAP-43 and nestin positive, but didn't express glial astrocyte marker GFAP.Conclusion rMSCs can be induced to differentiate into neuron like cells with Ligustrazin hydrochloride in vitro.
10.EXPRESSION AND ELECTROPHYSIOLOGICAL CHARACTERISTICS OF L-TYPE CALCIUM CHANNEL IN RAT BONE MESENCHYMAL STEM CELLS
Acta Anatomica Sinica 1953;0(01):-
Objective To explore the electrophysiological characteristics and the expression of mRNA and protein of L-type calcium channel in rat bone mesenchymal stem cells(MSCs). Methods MSCs were isolated,cultured and purified.RT-PCR was used to detect the mRNA expression of ?1C,?1D,?1H,?1S.The protein expression of L-type calcium channel(?1C) was testified by immunohistochamical.Ion currents were recorded in MSCs using whole-cell patch clamp technques.Results CD29,CD44,CD106 expressed in about 93% MSCs and CD14,CD34,CD45 expressed negatively.A high expression of mRNA in ?1C was detected by RT-PCR but no expressions were observed in ?1D,?1G,?1H,?1S.Immunofluorescent double labeling showed an expression of ?1C subunits in MSCs.Moreover,inward currents were recorded in 16 of 36 cells using whole-cell patch clamp techniques.The currents were activated around-30?mV and peaked at 0 to 10?mV and were blocked by nifedipine(10??mol/L).These cells had larger currents with Ba~(2+)(10?mmol/L) in bath solution than with Ca~(2+)(2?mmol/L).Conclusion The results indicated that adult rat MSCs expresse functional L-type calcium channels.It is possible that this channel plays a role on the proliferation and differentiation of MSCs.