Objective:To investigate the effects of sodium selenite on the cell apoptosis of K562 cells and to elucidate its molecular mechanisms.Methods:K562 cells were treated with various concentrations of sodium selenite at different time points,and then MTT assay was employed to evaluate the effects of sodium selenite on the proliferative inhibition of K562 cells.MTT assay was employed to evaluate the effects of sodium selenite on the proliferative inhibition of K562 cells.Electronmicroscopy,and TUNEL were performed to confirm the apoptosis of K562 cells,RT-PCR was employed to analyze the mRNA expression levels of Bcl-2 and Bax.Colorimetric method were used to measure the activities of caspase-3 of K562.Results:Sodium selenite could inhibit proliferation of K562 cells and induce them to undergo apoptosis.RT-PCR results showed that sodium selenite could decrease the mRNA expression level of Bcl-2 and increase the level of Bax of K562 cells which had been treated with sodium selenite for 48h significantly,and the activity of caspase-3 elevated remarkably too.Compared with the control group,the expression levels of Bcl-2,Bax and acivity of caspase-3 in 20?mol/L sodium selenite treatment group were markedly changed(P

Objective To optimize the extraction conditions for total flavonoids from Cibotium barometz by response surface meth-od(RSM). Methods According to the center combination of Box-Benhnken,using the RSM,the effects of ethanol concentration,the ratio of solid to liquid,the extraction time,and the extraction frequency were studied by central composite design. Results The opti-mal conditions of extraction were as follows:60％ethanol,the ratio of solid to liquid 1:40,refluxing and extracting twice,and 1.5 h for each time. Conclusion The actual extraction yield was 1.44％. The method of extraction has higher extraction efficiency than other methods and can provide a basis for the industrial production of the total flavonoids from S. barometz.

Retrospective studies of duodenal polyps have shown a prevalence of 0.3-4.6% in patients referred to upper gastrointestinal endoscopy, and histologic classification have been inconsistent. A prospective consecutive study was carried out in 3,871 patients referred to diagnostic endoscopy, Sixteen patients had polyps in the first part of duodenum, for a prevalence 0.41%(0.28-0.53%, 95% confidence interval). Fourteen polyps were either inflammatory(thirteen polyps) or ectopic gastric mucosa(one polyp). Two hyperplasitc polyps were founded. All polyps were benign and sessile, and most of polyps(75%) were solitary.
Classification ; Duodenum ; Endoscopy* ; Endoscopy, Gastrointestinal ; Humans ; Polyps* ; Prevalence* ; Prospective Studies

We studied arterial oxygen desaturation, using a pulse oximeter, in 132 patients undergoing diagnostic upper gastrointestinal endoscopy to obtain predictive factors of the change. The baseline arterial oxygen saturation (SaO2) level was 98.8+/- 1.2%. During the procedure, oxygen desaturation (SaO2>95%) was found in 90.2% of the patients, Mild oxygen desaturation (95>SaO2>90%) was found in 9.8% of the patients, and there was no severe oxygen desaturation(SaO2<90%). Age(P=0.52), gender(P =0.48), smoking(P =0.71), body mass index(P =0.32), and endoscopy time(P = 0.68) was not related to the degree of oxygen desaturation. These results suggest that oxygen desaturation, which may rarely induce serious cardiopulmonary events, is not frequently observed during non-sedated diagnostic upper endoscopy.
Endoscopy ; Endoscopy, Gastrointestinal* ; Humans ; Oxygen*

No abstract available.
Restraint, Physical*

Objective To study the pathological characteristics of delayed xenograft rejection and the expression of donor ICAM-1 in mouse-to-rat cardiac xenografts. Methods BALB/c mice and Lewis rats served as donors and recipients respectively. The model of mouse-to-rat heterotopic heart xenotransplantation was established. The cardiac xenografts were harvested at 12 h, 24 h, 36 h, 48 h after transplantation and at the time when no pulsations could be detected in the transplanted heart respectively. The normal BALB/c mouse hearts were harvested as control group. The grafts were col lected to receive pathological and immunohistochemical examinations as well as to detect the level of ICAM-1 mRNA in the xenografts. Quantitive measurement of ICAM-1 expression in the grafts was done by using multimedia pathology imaging analysis system. RT-PCR products of xenografts were separated by agrose gels and the densities of the bands were determined by density scanning. Results The pathologic examination of xenografts showed hyperemia, hemorrhage with inflammatory cells infiltrated at 12 h after transplantation and they became more and more serious as time went on. The pathologic examination of rejected xenografts showed widespread intravascular thrombosis, hyperemi-a, hemorrhage, coagulative necrosis with a large number of inflammatory cells infiltrated. The stained color of vascular endothelial cells and cardiac myocytes was significantly more intensive in the xeno-grafts than that of normal BALB/c mouse hearts in the control group. The relative density values (ICAM-1/?-actin) were also significantly higher in the xenografts than that of the control group. Conclusion ICAM-1 expression in the xenografts was up-regulated, which was related with the development of the delayed xenograft rejection.

Long noncoding RNAs (lncRNAs) are non-protein coding transcripts longer than 200 nucleotides. Most lncRNAs have pro-nounced oncogenic effects associated with tumorigenesis and progression, promoting the proliferation, migration, invasion, and me-tastasis of tumor cells. The specific lncRNAs expression in particular types of cancers makes them promising diagnostic and prognostic biomarkers. Currently, studies on lncRNAs expression, functions, and mechanisms have attracted considerable attention in cancer re-search. However, these studies mainly focus on epitheliogenic malignant tumors. In this review, we outline the current state of infor-mation on lncRNAs and research progress on its role in haematopoietic and lymphoid tissue tumors.

Purpose:To investigate p53 gene mutation, CD44V6 expression and their relationship with metastasis of ovarian carcinoma in tumor diagnosis. Methods:PCR SSCP with silver staining was used to detect the mutation of p53 gene; by using Southern blot and image analysis, the quantitative and qualitative expression of CD44V6 were also determined. Results:The positive percentage of CD44V6 expression and p53 gene mutation was not detectable in any of the normal ovarian specimens but in the benign tumors, non metastasizing and metastasizing carcinomas it was 10%, 75%, 88% and 5%, 40%, 60% respectively. The mean dark density of each band in these four groups(mentioned above) was 85.25?23.16, 817.11?126.5, 3820.14?289.43 and 10132.92?1521.20 respectively. The expression of CD44V6 of metastasizing carcinomas was higher than that of non metastasizing group. Conclusions:The expression of CD44V6 is related to tumor metastasis; the positive percentage of CD44V6 is higher than that of p53 gene mutation in the group of metastasizing and non metastasizing tumors; Compared to p53 gene mutation, CD44V6 is a better marker for tumor metastasis.

OBJECTIVE:To prepare aspirin gastric floating capsule and to study its in vitro drug-release characteristics.METHODS:Aspirin gastric floating capsule was prepared with aspirin as model drug,and with hydroxypropyl methylcellulose(HPMC)under different viscosity and different quantity and carbopol as main excipients.The release rate of the capsules was determined.RESULTS:When the optimal ratio between HPMC K4M and HPMC K15M was3to1,then the in vitro drug release of aspirin gastric floating capsule within0h～10h conformed to apparent zero-order kinetics with rate constant Kr at10.3%/h and release parameter at0.6173.CONCLUSIONS:The prepared capsule has a marked slow-release effect,the quantity and the viscosity of HPMC were the main factors affecting the release rate of gastric floating capsule,the in vitro drug release characteristics were associated with the joint action of bulk erosion and drug diffusion.

OBJECTIVETo investigate the effect of Metformin on the proliferation and collagen synthesis of the human keloids fibroblasts as well as the effect on phosphorylation of Akt/FoxO1 signal transduction pathway.

METHODSFibroblasts of keloid were divided into control group treated with medium solution and experimental groups treated with different concentrations of Metformin. 48 h later CCK-8 assay was adopted to evaluate cell survival; Western blot was performed to detect the Akt and FoxO1 phosphorylation; and Hydroxyproline reagent kit was used to detect the collagen synthesis.

RESULTSWith different concentrations (30, 60, 90, 120 mmol/L) of Metformin, the absorbance of cultured keloid fibroblasts detected by CCK8 assay decreased by (13.30 ± 2.04)%, (22.64 ± 4.70)%, (54.00 ± 5.34)% and (63.12 ± 3.48)%. The growth of fibroblasts was suppressed by Metformin in a dose-dependent manner. It showed that the level of phoshpo-akt and phoshpo-foxOl in keloids fibroblasts in experimental groups was lower than that in the control group and the collagen synthesis were also decreased in experimental groups, all in a dose-dependent manner (P < 0.05, P < 0.01).

CONCLUSIONSMetformin can effectively inhibit the proliferation and collagen synthesis of the human keloids fibroblasts in vitro, which may be associated with the suppression of phosphorylation of Akt/FoxO1 signaling pathway

Cell Proliferation ; drug effects ; Collagen ; biosynthesis ; Dose-Response Relationship, Drug ; Fibroblasts ; cytology ; drug effects ; metabolism ; Forkhead Box Protein O1 ; Forkhead Transcription Factors ; metabolism ; Humans ; Keloid ; pathology ; Metformin ; pharmacology ; Phosphorylation ; Proto-Oncogene Proteins c-akt ; metabolism ; Signal Transduction ; drug effects