The theory of planned behavior is used to study physician's behavior of clinical pathway compliance,on which the model of decision making mechanism for such compliance was built.This aims at exploring the relationship between the behavioral risk factors,behavioral belief and behavioral intention, and the occurrence,and recommend on further regulation of physicians'behavior and policies in hospital management.

By searching relevant data from the PubMed database, Chinese National Knowledge Infrastructure (CNKI) database and Wanfang database, a comprehensive analysis and review regarding acupuncture for cerebral ischemia reperfusion injury (CIRI) in recent 10 years were performed. The results showed that acupuncture could inhibit the inflammatory reaction, reduce oxidative stress injury, restrain brain edema formation, inhibit apoptosis, promote neural and vascular regeneration, etc. Acupuncture methods used included electroacupuncture, scalp acupuncture, eye acupuncture and "consciousness-restoring resuscitation needling", etc. The existing problem was that the intervention action of acupuncture was mainly focused on inhibiting inflammatory reaction and oxidative stress injury, and the study on apoptosis and neural and vascular regeneration was needed. It is suggested that from the aspect of multiple target points, the intervention mechanism of acupuncture for CIRI should be systemically studied in the future, which could provide new idea for clinical diagnosis and treatment on ischemic cerebrovascular diseases.
Acupuncture Therapy ; history ; Animals ; Apoptosis ; Brain Ischemia ; history ; surgery ; History, 21st Century ; Humans ; Oxidative Stress ; PubMed ; Reperfusion Injury ; etiology ; history ; physiopathology ; therapy

Objective: To study the effect of down-regulation of eukaryotic translation initiation factor 4E (EIF 4E ) induced by the siRNA expression plasmid on the proliferation and cell cycle of human breast cancer cell line MDA-MB-231. Methods: The siRNA expression plasmid pGPU6/GFP/Neo-EIF4E was constructed and then transfected into breast cancer MDA-MB-231 cells by LipofectAMINE2000. The expressions of EIF4E and cyclin D1 mRNAs and proteins were examined by RT-PCR, Western blotting and the immunocytochemistry method, respectively. The MTT method, plate colony formation assay and flow cytometry were performed to detect the proliferation ability, cell colony formation rate and cell cycle distribution of MDA-MB-231 cells transfected with siRNA expression plasmid. Results: The siRNA expression plasmid pGPU6/GFP/Neo-EIF4E was constructed successfully. The expressions of EIF4E and cyclin D1 mRNAs and proteins were all decreased markedly after transfection with pGPU6/GFP/Neo-EIF4E (P <0.05). The growth of MDA-MB-231 cells in the pGPU6/GFP/Neo-EIF4E-tranfected group was inhibited and the cell colony formation rate was statistically decreased (P<0.05) as compared with those in the blank control and the pGPU6/GFP-transfected groups. The result of flow cytometry showed that the percentage of cells in G1 phase in the pGPU6/GFP/Neo-EIF4E-tranfected group was higher than that in the blank control group (71.30%±0.47% vs 53.10%±0.43%); however, the percentage of cells in S phase was signifcantly lower (12.53%±0.13% vs 26.47%±0.38%, P <0.05). Conclusion: EIF 4E siRNA can downregulate EIF 4E gene expression in human breast cancer MDA-MB-231 cells, suppress cell proliferation to some extent, and increase the number of cells in G1 phase with a decreased number of cells in S phase. EIF 4E gene may become one of the important molecular targets to breast cancer. Copyright© 2011 by the Editorial Board of Tumor.

Adolescent ; Child ; Epidural Abscess ; diagnosis ; surgery ; Female ; Humans ; Male ; Spinal Diseases ; diagnosis ; surgery

Objective To explore the clinical effect of periodontic treatment in type 2 diabetic patients (T2DM ) with chronic periodontitis on basis of hypoglycemic therapy. Methods From September 2011 to January 2014 ,120 T2DM patients with chronic periodontitis were selected and divided into treatment group and control group with 60 cases in each group. Patients in control group were treated with conventional hypoglycemic therapy. Patients in treatment group were treated with initial periodontal therapy additionally on basis of conventional hypoglycemic therapy. Probing depth (PD) ,attachment loss (AL) ,plaque index (PLI) ,bleeding index (BI) and hypersensitive C reactive protein (hsC‐RP) levels were compared before and after 6 weeks treatment. Results PD ,AL ,PLI ,BI ,and hsC‐RP levels had no significant difference between two groups before treatment (P>0.05). PD ,AL ,PLI ,BI and hsC‐RP levels after treatment in treatment group were all significantly decreased [PD :(2.11 ± 0.28) vs (2.51 ± 0.68) and (2.42 ± 0.31) mm ;AL :(3.18 ± 0.41) vs (3.58 ± 0.83) and (3.49 ± 0.58) mm ;PLI:(0.41 ± 0.21) vs (0.96 ± 0.33) and (0.78 ± 0.26);BI:(0.97 ± 0.32) vs (2.65 ± 1.21) and (1.14 ± 0.30);hsC‐RP :(1.02 ± 0.63) vs (3.29 ± 2.88) and (2.72 ± 1.51) mg/L] as compared with those before treatment in treatment group and those after treatment in control group (P<0.05). Conclusion Levels of inflammatory factors will decrease when periodontic treatment is added to the antidiabetic therapy in T 2DM patients with chronic periodontitis.

Lobaplatin is the third generation platinum drug.Many studies on pharmacodynamics have confirmed that lobaplatin can block the cell cycle and induce cell apoptosis,and thus inhibits tumor cell proliferation effectively.Lobaplatin is not cross-resistant to cisplatin.Besides,it has good curative effect to various kinds of cancer patients with mild adverse reactions,and the patients are well tolerable to it.

Objective:To investigate the effects of IL-2 on the proliferation activity and HCG secretion of first trimester human trophoblastic cells in vitro. Methods:Growing -well 4th generation trophoblastic cells were selected,cultured for 48 h with IL-2 of different concentration. The MTT colorimetric assay was used to analyze the proliferation activity, and radioimmunoassay was used to analyze the secretion of HCG. Results:①Low concentration (100 U/ml,400 U/ml) IL-2 promoted proliferation activity and HCG secretion (P

For a valid control of the finance management, the management system is developed again aiming at finance related control links and key control points. In this way, the control is rigorous and integral with simple procedures for check and examination. The key control point is set oriented to note management. Real-time operation is controlled. Post output processing is controlled. With business income and business expenditure as the main line, the control process of the related links are built up. Computer maintenance system is controlled.

Objective To observe the effect and mechanism of magnesium on nigral dopaminergic neurons in rats with Parkinson's disease(PD). Methods The PD rats were prepared by unilateral injection with 6-hydroxydopamine(6-OHDA) in the nigra and then divided into magnesium sulfate group,Madopar group,mixed group(magnesium sulfate+Madopar) and control (normal saline) group,and recived corresponding therapy by gastric perfusion for 28 d.The behavior change was observed. At the damage side,the number of tyrosine hydroxylase(TH) positive neurons in nigra was detected by immunohistochemistry; the activities of superoxide dismatase(SOD),glutathione peroxidase (GSH-Px) and the level of malondialdehyde(MDA) in striatum were measured by biochemistry method,the expression of caspase-3 mRNA and the protease of nuclear factor(NF)-?B P65 in nigra were detected by RT-PCR and Western Blot. Results After treatment,the stable contralateral rotation was found only in mixed group. Compared with other groups,the number of TH positive neurons in mixed group was significantly increased (allP

The quantity or function deficiency of blood vessel grafts hinders many diseases from proper treatment,so tissue engineered blood vessels have gained high attentions.As a crucial component of the tissue engineered blood vessels,how the seed cells can obtain the physiological functions as normal endothelial cells and smooth muscle cells has been focused on.Stem cells are multi-potential and are regarded as proper seed cells.However,it is still a question that which stem cells among a variety of stem cells is the most suitable seed cells for tissue engineered blood vessels.In this review,we make a comparison among kinds of stem cells such as haemopoietic stem cells,bone mesenchymal stem cells and adipose-derived stem cells.We also state their characteristics respectively,and intent to acquaint with their function comprehensively.Thus,this review may provide some references for further studies.