OBJECTIVE:To observe the therapeutic effect of IF ?-2b plus compound glycyrrhizin on chronic hepatitis C. METHODS:56cases of chronic hepatitis C were divided into trial group(30cases)and control group(26cases).Trial group received IF ?-2b +compound glycyrrhizin and control group received IF ?-2b alone.The therapeutic course was24weeks in both groups.RESULTS:There was significant difference in recoversion rate of ALT between two groups either at the end of ther?apeutic course or24weeks after treatment(P

Objective To investigate The effect of TAM(2.5 uM)in the chemotherapy of HCC and its mechanism. Methods An adriamycin-resistant human HCC cell subline(Hep-3B/ADM)was established,through exposure to gradually increased concentration of ADM.Drug sensitivity was measured by MTT.Modulation of ADM-induced apop- tosis of Hep-3B/ADM cells was assessed by Flow cytometry(FCM).PKC activity was determined by applying compet- itive protein binding method.Results TAM(2.5uM )decreased the IC50 of ADM from 1.64?g/mL to 0.23?g/mL in the Hep-3B/ADM cells.TAM exhibited a 2-fold potentiation on apoptosis caused by ADM in Hep-3B/ADM cells.With TAM,PKC activity was lower than that in control groups from 30 seconds to 24 hours.But this effect of TAM could be reversed by the co-treatment of the cells with TPA.Conclusion TAM(2.5 uM)significantly en- hanced ADM-induced cytotoxicity.The mechanism is that TAM potentiates the apoptosis induced by ADM through pro- tein kinase C inhibition.

C. Conclusion The optimal SINI TANG composition comprises three active components combined at clinical maximal dosage showed the best therapeutic efficacy, of which A is a key factor and B, C are necessary factors for the composition in SINI TANG.

Objective To compare the effects of hyperbaric oxygenation(HBO) and routine therapy on diabetic foot. Methods Thirty six subjects were randomized to HBO and routine groups. In HBO group,patients stayed in 0.25 MPa HBO chamber for 100 min,wearing masks and inhaling pure oxygen for 60 min,with 10 min interval.This treatment was performed once a day for 10 times as a course,altogether 2 courses for each patient.By means of hemorrheology and electromyograghy (EMG), and the color Doppler ultrasonograghy of lower extremities for detection of the microcirculation states of lower limbs, thirty six patients with diabetic foot were assessed. Results In HBO group all of patients showed that the abnormalities in hemorrheologic, EMG parameters, and the arteria lesions as revealed by Doppler ultrasonograghy were improved more or less compared with before the treatment( P

So far, there is no effective treatment for most of patients with sensorineural hearing loss. Many studies have shown that gene therapy has become more promising in terms of protecting the inner ear from these diseases. In recent years, inner ear gene therapy has become a research focus in the field of otology. It has not yet appeared a feasible technique for inner ear gene therapy in clinical application due to the particularity of the structure and function of inner ear. On the other hand, in order to avoid the adverse effects of carcinogenic and immunity caused by viral vectors, many scholars devote themselves to develop new gene vectors based on non-viral materials, such as liposomes, polymers and other nanomaterials. In recent years, many great progresses have been made in development and application of non-viral vectors, which showing an inspiring prospect. The research status, progress and prospects of non-viral vectors in inner ear gene therapy will be discussed in this review.
Animals ; Ear, Inner ; Gene Transfer Techniques ; Genetic Therapy ; Genetic Vectors ; Humans

Cerebral amyloid angiopathy is closely associated with intracerebral hemorrhage. In recent years, a further understanding of etiology, pathogenesis, diagnosis and treatment on cerebral amyloid angiopathy-related intracerebral hemorrhage has been achieved.

Objective To investigate the changes and clinical significance of the platelet parameters in tumor patients before or after radiochemotherapy.Methods The platelet parameters(PLT,PCT,MPV,PDW)were detected in 65 cases of tumor patients before and after radiochemotherapy by the Sysmex XN-1000 automatic hemacytology analyzer.Then the detection results were com-pared with those in the control group.Results Compared with the control group,PLT and MPV before therapy in the tumor pa-tients were significantly increased(P <0.01),but PCT and PDW had no statistical differences between the two goups(P >0.05). PLT,PCT and MPV after radiochemotherapy in the tumor patients were significantly lower than those before treated(P <0.01 ), but no statistically significant difference in PDW was found(P >0.05).Conclusion The measuration of platelet parameters in the tumor patients with radiochemotherapy has certain clinical significance to observe the status of the bone marrow suppression and es-timate the state of illness.

BACKGROUND:Nonunion is a common clinical problem of bone fracture. For decades, with the help of
internal/external fixation materials, popularization of microsurgical technique, innovation of bone graft materials, especial y molecular biology techniques, breakthrough progress has been made in the treatment of nonunion. OBJECTIVE:To summarize the research progress of treatment in nonunion and to provide better technology theory and choices of method for the treatment of nonunion in future.
METHODS:The PubMed database and CNKI database were retrieved by the first author for the articles related to reason and treatment method of nonunion published from January 1990 to May 2013. The key words were
“fracture nonunion, treatment, progress”. For the articles in the same field, those published recently or in authorized journals were selected.
RESULTS AND CONCLUSION:The repetitive and old articles were eliminated, and final y 48 articles were selected for further analysis. At present, two methods can be used in the treatment of nonunion, including
nonsurgical method and surgical method, the surgical method is utilized more frequently and more efficiency in clinic. Fracture healing is the complex process involving various aspects, and once the nonunion occurred,
different patients should be treated with different methods, and the combination of various surgical and
nonsurgical methods can be used if necessary to obtain satisfactory effect. But al the treatment methods should pay attention to soft tissue protection.

Objective To investigate the relationship between the expressions of TMPRSS4 and clinical pathologi-cal parameters in gastric carcinoma. To explore its significance in judging the prognosis of patients. To analyze the expression of TMPRSS4 in the epithelial-mesenchymal transition. Methods The expressions of TMPRSS4, E-cad-herin and Vimentin were detected by immunohistochemistry ( SABC) in 100 cases of gastric carcinoma specimens and corresponding adjacent normal tissue. Results In gastric carcinoma, TMPRSS4, E-cadherin and Vimentin positive rate was 47%,45%,37%. In adjacent tissues,their positive rate was 9%,30%,4%. The differences be-tween tumor tissues and adjacent tissues had statistical significance ( P<0.05 ) . High expression of TMPRSS4 cor-related significantly with lymph node metastases and TNM stage (P<0.05), but not with gender, age, size, tumor histological type,depth of invasion (P>0.05). The 5-year survival rate of patients with high TMPRSS4 expression was significantly lower than that in patients with low expression. The high TMPRSS4 expression was significantly correlated in gastric carcinoma accompanied by low expression of E-cadherin (rs = -0.207,P=0.038) and high Vimentin expression (rs=0.233,P=0.020). Conclusion The expression of TMPRSS4 is closely related to the biological characteristics in gastric carcinoma,detection the expression of TMPRSS4 is valuable in predicting tumor prognosis,invasion and metastasis. TMPRSS4 may promote invasion, metastasis of human gastric carcinoma through epithelial-mesenchymal transition by reduce the expession of E-cadherin.

Objective To investigate the efficacy of rehabilitation in treatment of patients with hypertensive cerebral hemorrhage. Methods 64 patients with hypertensive intracerebral hemorrhage according to whether the implementation of the rehabilitation treatment of patients were randomly divided into two groups, the observation group and the control group,and each group(32 cases) was observed at two of the limb motor function,activities of daily living and quality of life scores. Results There were high blood pressure in patients with cerebral hemorrhage FuglMeyer score, Barthel index score, QQL quality of life score after 4 weeks after treatment, scores were significantiy improved by the test showed significant difference( all P ＜0. 05). Conclusion Active rehabilitation in patients with hypertensive intracerebral hemorrhage could improve the patients' limb motor function and daily life, improve quality of life of patients.