Objective　To evaluate the status of urological laparoendoscopic single-site surgery (LESS) technique in China.　Methods　We had conducted a systematic review of literatures of urological laparoendoscopic single-site surgery published from Chinese institutions using WanFang,CNKI,VIP,CBM,GoogleScholar and MEDLINE databases.All relevant articles were selected with consensus and analyzed according to the inclusion and exclusion criteria.　Results　Overall,205 articles from 66 different centers all over China published between January 2009 and June 2012 were included in the analysis. Of which,there were 26 articles of science citation index papers and 179 articles from Chinese academic journals.The included literature was mainly composed of case series reports ( 133 articles,64.9％ ),comparison studies (13 articles,6.3％ ),reviews or comments (20 papers,9.8％ ),nursing related papers (28 pieces,13.7％ ),animal surgery studies (6 articles,2.9％ ),and training (5 articles,2.4％ ).A total of 1790 clinical cases were reported during the study period,56.9％ of these were procedures done in the upper urinary tract,only 5.8％ were in the lower urinary tract,and 37.3％ were genital or other procedures.Procedures of tumor-related and reconstruction required accounted for 18.8％ and 18.7％,respectively.Pure single-port technique was applied on 58.3％ of cases.In cases of a single-port platform used,45.6％ used the commercially available devices and 54.4％ used homemade devices.Transperitoneal accesses were adopted in 59.5％ of cases and the other 40.5％ were performed through extraperitoneal approaches.The umbilicus was used as the site of access in 58.5％ of cases.A total of 25 indications were reported,including adrenalectomy,nephrectomy and ureterolithotomy,etc. in the upper urinary tract,radical cystectomy,radical prostatectomy and transvesical enucleation of the prostate,etc.in the lower urinary tract,and varicocelectomy,orchidopexy and others. The three most often done procedures were varicocelectomy (601　cases,33.6％ ),renal cyst decortication (408 cases,22.8％ ) and adrenalectomy ( 179 cases,10.0％ ).The overall conversion rate was 5.5％,and complications were encountered in 3.4％ of cases.　Conclusions Laparoendoscopic single-site surgery has been widely adopted by Chinese urologists and largely covered the spectrum of conventional urologic laparoscopic procedures,with most of these being non-reconstructive operations for the treatment of benign diseases.In large,we are still under the initial stage of LESS application in urology.And more work on creative innovation and well-designed studies are needed to further evaluate its role in the urological minimally invasive surgery.

Objective To compare biomechanical performances of parallel plate fixation and vertical plate fixation for better clinical treatment of humeral intercondylar comminuted fractures (HICF).Methods Models of T-shaped fracture were created using an osteotome at the distal end of humerus in 6 specimens of upper limb in 3 human adult cadavers.The 2 fracture models of left and right humeri in the same cadaver were respectively fixated with parallel plating and vertical plating.On a biomaterial-testing machine,a vertical load was applied onto the fixated distal humerus at a speed of 5 N/s till it reached 2000 N.The loading was repeated 3 times.Vertical compression displacement,intercondylar separating displacement,deformation of medial and lateral condyles and vertical compression stiffness were documented for statistic analyses. Results Under the same load,parallel plating led to significantly less compression displacement,separating displacement and deformation of condyles( P ＜ 0.05) but significantly greater axial compression stiffness( P ＜ 0.05) than vertical plating. Conclusion In biomechanics,parallel plate fixation has better performance than vertical plate fixation for HICF.

BACKGROUND: The wear resistance and hardness of artificial tooth would affect repairing effects in clinic. However, there is lack of professional standard for resin teeth in China.OBJECTIVE: To provide a theoretic foundation for the choosing of the resin teeth in clinic via comparing the wear resistance and hardness among 7 different resin teeth.DESIGN, TIME AND SETTING: A contrast observation. The experiment was performed at the laboratory of Mechanical Engineering College, Shanghai Jiao Tong University from November 2003 to May 2004.MATERIALS: A total of 7 kinds of different resin teeth were selected, namely, hard multi-layer synthetic resin teeth (A); multi-layer synthetic resin teeth (B); synthetic resin teeth (C); EMDURA resin teeth (D); three-layer synthetic resin teeth (E); optostar four-layer synthetic resin teeth (F); and Cosmo HXL (G).METHODS: The wear test was processed by a pin-plate wear machine. The result was measured by the weight loss through wearing and the surfaces of the wear scar were observed by a scanning electronic microscope (SEM); hardness was measured by Knoop hardness. Meanwhile, the relativity between the hardness and wear resistance of resin teeth was analyzed.MAIN OUTCOME MEASURES: The wear resistance and hardness of resin teeth.RESULTS: According to the weight loss and SEM examination, the wear resistance of the different resin teeth was D>A>G>E>B>F>C; and the hardness of different resin teeth was D>A>G>E>F>B>C. The correlation coefficient between the weight loss and hardness was -0.888 (P < 0.01).CONCLUSION: The variation of the wear resistance of the resin teeth may influenced by their different components, molecular weight and manufacturel methods. There are positive correlation between the hardness and wear resistance of resin teeth.

Objective To investigate the efficacy of rehabilitation in treatment of patients with hypertensive cerebral hemorrhage. Methods 64 patients with hypertensive intracerebral hemorrhage according to whether the implementation of the rehabilitation treatment of patients were randomly divided into two groups, the observation group and the control group,and each group(32 cases) was observed at two of the limb motor function,activities of daily living and quality of life scores. Results There were high blood pressure in patients with cerebral hemorrhage FuglMeyer score, Barthel index score, QQL quality of life score after 4 weeks after treatment, scores were significantiy improved by the test showed significant difference( all P ＜0. 05). Conclusion Active rehabilitation in patients with hypertensive intracerebral hemorrhage could improve the patients' limb motor function and daily life, improve quality of life of patients.

Cerebral amyloid angiopathy is closely associated with intracerebral hemorrhage. In recent years, a further understanding of etiology, pathogenesis, diagnosis and treatment on cerebral amyloid angiopathy-related intracerebral hemorrhage has been achieved.

Supernumerary tooth is common,but symmetrical supernumerary tooth on both sides is rare.A case of symmetrical supernumer-ary tooth of maxillary posterior teeth area is reported in this paper.

Objective To investigate the relationship between the expressions of TMPRSS4 and clinical pathologi-cal parameters in gastric carcinoma. To explore its significance in judging the prognosis of patients. To analyze the expression of TMPRSS4 in the epithelial-mesenchymal transition. Methods The expressions of TMPRSS4, E-cad-herin and Vimentin were detected by immunohistochemistry ( SABC) in 100 cases of gastric carcinoma specimens and corresponding adjacent normal tissue. Results In gastric carcinoma, TMPRSS4, E-cadherin and Vimentin positive rate was 47%,45%,37%. In adjacent tissues,their positive rate was 9%,30%,4%. The differences be-tween tumor tissues and adjacent tissues had statistical significance ( P<0.05 ) . High expression of TMPRSS4 cor-related significantly with lymph node metastases and TNM stage (P<0.05), but not with gender, age, size, tumor histological type,depth of invasion (P>0.05). The 5-year survival rate of patients with high TMPRSS4 expression was significantly lower than that in patients with low expression. The high TMPRSS4 expression was significantly correlated in gastric carcinoma accompanied by low expression of E-cadherin (rs = -0.207,P=0.038) and high Vimentin expression (rs=0.233,P=0.020). Conclusion The expression of TMPRSS4 is closely related to the biological characteristics in gastric carcinoma,detection the expression of TMPRSS4 is valuable in predicting tumor prognosis,invasion and metastasis. TMPRSS4 may promote invasion, metastasis of human gastric carcinoma through epithelial-mesenchymal transition by reduce the expession of E-cadherin.

It was argued whether bisphenol A(BPA) can induce reproductive toxicity to male,so in the present paper the recently published articles on the reproductive toxicity induced by BPA to male were reviewed.It was indicated that BPA has no obvious embryotoxicity,but BPA can directly induce the reproductive toxicity to male reproductive system,and the testis and prostate were the main target organs of BPA.Even low level BPA exposure could produce toxic effects.

Objective:To determine whether alpha-fetoprotein(AFP) messenger RNA(mRNA),a specifically hepatocyte-expressed gene,could be a marker of metastasis in hepatocellular carcinoma. Methods:Peripheral blood samples were obtained from 30 patients with hepatocellular carcinoma (HCC).A sensitive reverse transcription polymerase chain reaction( RT-PCR) technique was used to detect the human AFP mRNA in the blood.Results:Of the 30 HCC patients,14(46.7%) patients had positive AFP mRNA.The presence of AFP mRNA correlated with the TNM clinical stages,the size of tumor,extrahepatic metastasis or with portal vein carcinothrombosis(P0.05).Conclusion:The positive of AFP mRNA in the peripheral blood can indicate the presence of hematogenous metastasis in patients with HCC.

So far, there is no effective treatment for most of patients with sensorineural hearing loss. Many studies have shown that gene therapy has become more promising in terms of protecting the inner ear from these diseases. In recent years, inner ear gene therapy has become a research focus in the field of otology. It has not yet appeared a feasible technique for inner ear gene therapy in clinical application due to the particularity of the structure and function of inner ear. On the other hand, in order to avoid the adverse effects of carcinogenic and immunity caused by viral vectors, many scholars devote themselves to develop new gene vectors based on non-viral materials, such as liposomes, polymers and other nanomaterials. In recent years, many great progresses have been made in development and application of non-viral vectors, which showing an inspiring prospect. The research status, progress and prospects of non-viral vectors in inner ear gene therapy will be discussed in this review.
Animals ; Ear, Inner ; Gene Transfer Techniques ; Genetic Therapy ; Genetic Vectors ; Humans