Objective:Select a suitable macroporous resin for the purification technic of total saponins from Panacis Japonici Rhizoma and determine the parameter of purification technic. Methods:Made the content of total saponins as the index, used static adsorption test and combined the adsorption kinetic parameters to select the type of macroporous resin. By using dynamic adsorption experiment to investigate the technical parameters of the purified macroporous resin extracted from Panacis Japonici Rhizoma. Then the preparation technic of the total saponins of Panacis Japonici Rhizoma was determined. Results:The D101 macroporous resin could absorpt and desorpt total saponins of Panacis Japonici Rhizoma effectively. The optimal purification parameters were as follow: the loading mass concentration was 0.1 g/ml (based on crude drug), and the loading volume was 100 ml (which means the loading volume of resin per ml was equivalent to 3.3 grams of crude drug). During the elution process, distilled water (3 BV) and 20% ethanol (3 BV) were used to remove impurity, and then 70% ethanol elution (6 BV) was used to enrich the total saponins. The flow rate of loading and elution was 0.5 ml/min. The transfer rate of total saponins could reache 85.6%. Conclusion:The D101 macroporous resin can effectively enrich and purify the total saponins of Panacis Japonici Rhizoma, which provides the scientific basis for the development and utilization of Panacis Japonici Rhizoma.

Objective To explore and discuss the factors for restricting the two-way referral system from the patient's perspective in order to promote the formation of hierarchical medical system in Changsha City.Methods The stratified random sampling method was adopted to divide the Chansha City into the 3 grades of high,middle and low according to the regional economical development situation,2 districts in one grade,including 6 districts.Then each district was redivided into high,middle and low grades according to the economic situation,2 basic medical institutions were selected from each grade.A total of 360 patients were randomly sampled for conducting the questionnaire investigation.The patients and doctors specially participating in the two-way referral conducted the deep interview.Results The patients choosing to primary hospital for first visit accounted for 60.9％.The awareness degree of patients to two-way referral system was 21.1％,and the two-way referral service satisfaction in the patients with two-way referral was 85.7 ％.The patients' will of transfer treatment from primary hospital to superior hospital in case of disease condition need was 82.9 ％.The patients' will for conducting rehabilitation treatment from superior hospital to primary hospital was 77.6 ％.Conclusion Few patients choose primary hospital for first visit.The signing rate of family contract services is low and awareness degree of two-way referral system is not high.

Primary hospitals are engaged in such responsibilities as diagnosis and treatment of endemic diseases and common illnesses of the locality, as well as relevant research work which bears more prominent importance than ever before. Given the importance of research, the hospitals are mostly plagued by such setbacks as obsolete research management concepts, poor experiences, poor perception of research, poor professional competency and lack of academic exchange among the medical workers. A primary hospital had set up a novel management mechanism, featuring " full-staff involvement, full-process supervision and full-dimensional guidance" , and " tight formal examination and tight content examination" . This mechanism has been in place since 2016 in research project management practice, achieving such progresses as significant rise in the number of research project applications, that of approved projects, and project implementation capabilities.Future improvements in this regard should be made in higher pertinence, optimized management flow and better research service system.

Objective:To compare the variability of midpalatal suture maturation ratio of skeletal class Ⅰ malocclusion adults between genders,among positions and vertical skeletal patterns.Methods:173 skeletal class Ⅰ malocclusion adult patients were included and divided into 6 groups according to GoGn-SN Angle and gender:dolichofacial group(male 22,female 24)with GoGn-SN＞37.7°,me-sofacial Group(male 30,female 33)with 27.2°≤GoGn-SN≤37.7° and brachyfacial group(male 33,female 31)with GoGn-SN＜27.2°.The midpalatal suture maturation ratio in the corresponding area of anterior,medial and posterior region were measured and calculated by Mimics 21.0 software,the overlap of the anterior nasopalatine canal with the measurement site was recorded.Results:In the comparison of different genders,there was a statistically significant difference between males and females in the brachyfacial an-terior region(P＜0.001).In the comparison of different positions,except the females among the anterior,middle and posterior regions in brachyfacial group(P＞0.05),there were statistical differences in the fusion degree of the other parts of the palate suture(P＜0.05),and it gradually decreased from the posterior region to the anterior region.In the comparison of different vertical skeletal pat-terns,there was no statistical difference except the difference between the mesofacial and brachyfacial and dolichofacial groups in the middle area of males(P＞0.05),and the difference was significant among brachyfacial,dolichofacial and mesofacia groups in the ante-rior area of females(P＜0.05).Rates of anterior nasopalatine duct influence measurements were statistically significant in the compari-son between dolichofacial and brachyfacial males,and between brachyfacial males and brachyfacial females(P＜0.05),the remainder was not statistically significant(P＞0.05).Conclusion:In adult patients,the palatal suture is not completely fused,and palatal mi-croimplant anchorage should be avoided in the midpalatal suture.

Fourteen compounds were isolated from the 80% ethanol extract of Caragana stenophylla root, by using a combination of various chromatographic approaches, including silica gel sephadex LH-20 column chromatography, and preparative HPLC. On the basis of their physical and chemical properties and spectroscopic data, their structures were elucidated as 2-(4-hydroxy-3-methoxy lphenyl)-3-methoxyl benzofuran-6-ol (1), mucodianin C (2), isopterofuran (3), formononetin (4), afromosin (5), calycosin (6), acacetin (7), 3-O-methylkaempferol (8), liquiritigenin (9), isoliquiritigenin (10), variabilin (11), resveratrol (12), zhebeiresinol (13), and 2, 3-dicarboxy-6, 7-dihydroxy-1-(3', 4'-dihydroxy)-phenyl-1, 2-dihydronaphthalen (14). Compound 1 is a new benzofuran derivative, named as mucodianin S; compounds 2, 3, 11, 13, 14 were isolated from the genus Caragana for the first time, and compounds 4-10 were firstly isolated from Caragana stenophylla. MTT assay was used to determine their cytotoxicity of the isolated compounds against human tumor cell lines, and 2 showed cytotoxicity against human hepato cellular cancer (HepG2) and human cervical (HeLa) lines, with IC₅₀ values of (16.18±0.95), (3.75±0.08) μmol•L ⁻¹, respectively.