1.Clinical Result of Surgical Treatment of the Idiopathic Club Foot
SH LEE ; SW SUH ; WJ LEE ; SJ HONG
The Journal of the Korean Orthopaedic Association 1996;31(3):418-425
Authors reviewed clinical results of idiopathic club foot, of 52 patients have been treated by surgery and followed up more than 2 years at the Department of Orthopaedic Surgery, Korea University, Guro Hospital in order to evaluate the results after surgery for idiopathic club foot and the best time for surgical management. Materials consist of 3 groups regarding the surgical regimen:Group I was of one-stage postermedial release(58 feet/36 patients), Group II of posterolateral release(11 feet/7 patients)and Group III of Lichtblau osteotomy(10 feet/9 patients). Assessment was made by the radiologic(Beatson and Ponsenti's)and clinical(Preson and Fell's)criterias. From the review the following observations were obtained: 1. Percentage of the surgically treated feet was 30% in author's series(97 feet/327 feet). 2. The mean age at the operation was 1 year 11 months in group I, 1 year 11 months in group II, 6 years 2 months in group III and 3 years 4 months(2 months – 12 years) in all groups. 3. The clinical results evaluated by Preston's method were good in 32 feet(55%), fair in 16 feet(28%), poor in 10 feet(17%) for the group I, 58 feet and good in 4 feet(36%), fair in 4 feet(36%), poor in 3 feet(28%) for the group II, 11 feet and good in 6 feet(60%), fair in 2 feet(20%), poor in 2 feet(20%) for the group OOO, 10 feet. 4. The radiologic results evaluated by Beatson and Posenti's criteriae were good in 44 feet(55%), fair in 18 feet(23%), poor in 17 feet(22%) in all groups. 5. The success rate of primary surgical treatment for idiopathic club foot was 81% in all groups and 83% in PMR group. 6. Regarding age at operation, the best result was showed between 1 and 2 years old in PMR group. In conclusion, the surgery was the satisfactory method for the treatment of idiopathic club foot and the posteromedial release was showed better results than the posterolateral release group. Results according to age at operation in PMR group, the best results obtained between 1 year and 2 years old. The results in posterolateral release group was poor because the forefoot adduction deformity was persistant.
Congenital Abnormalities
;
Foot
;
Humans
;
Korea
;
Methods
2.Villoglandular adenocarcinoma of cervix:a clinicopathological study.
Zheng-cao LIU ; Lu ZHENG ; Yun-long HUO ; Xiang-hong YANG ; Ai-feng GAO ; Xiu-juan CUI
Chinese Journal of Pathology 2010;39(5):338-339
Adenocarcinoma
;
metabolism
;
pathology
;
surgery
;
Adenocarcinoma, Clear Cell
;
metabolism
;
pathology
;
Adult
;
CA-125 Antigen
;
metabolism
;
Carcinoembryonic Antigen
;
metabolism
;
Cervical Intraepithelial Neoplasia
;
metabolism
;
pathology
;
surgery
;
Cystadenocarcinoma, Serous
;
metabolism
;
pathology
;
Diagnosis, Differential
;
Female
;
Follow-Up Studies
;
Humans
;
Hysterectomy
;
Ki-67 Antigen
;
metabolism
;
Lymph Node Excision
;
Membrane Proteins
;
metabolism
;
Neoplasm Invasiveness
;
Uterine Cervical Neoplasms
;
metabolism
;
pathology
;
surgery
3.Effects of TGF-β1 on gene expression of connective tissue growth factor in lung fibroblasts.
Jian-Hua FU ; Hai-Ping YANG ; Li PAN ; Xin-Dong XUE ; Hong GAO
Chinese Journal of Contemporary Pediatrics 2011;13(1):36-39
OBJECTIVETo study the effects of transforming growth factor-β1 (TGF-β1) on the gene expression of connective tissue growth factor (CTGF) in cultured lung fibroblasts of embryonic rats in vitro.
METHODSWistar rats of embryonic 19 days were used for primary culture of lung fibroblasts (LFs). The cells in the experimental group were treated by different concentrations (1, 5 or 10 ng/mL) and different durations (12, 24 or 48 hrs) of TGF-β1 to stimulate the LFs. The cells in the control group were cultured in serum-free medium. RT-PCR method was applied to detect CTGF mRNA expression in LFs.
RESULTSCompared with the control group, the levels of CTGF mRNA in LFs in the experimental group increased significantly (P<0.05). CTGF mRNA expression gradually increased with increasing concentration and duration of TGF-β1 treatment (P<0.05).
CONCLUSIONSTGF-β1 can stimulate CTGF gene expression in LFs and increase CTGF gene expression in a dose-and time-dependent manner.
Animals ; Connective Tissue Growth Factor ; genetics ; Female ; Fibroblasts ; metabolism ; Gene Expression ; drug effects ; Lung ; cytology ; metabolism ; Pulmonary Fibrosis ; etiology ; RNA, Messenger ; analysis ; Rats ; Rats, Wistar ; Transforming Growth Factor beta1 ; pharmacology
4.Relationship of Ghrelin gene polymorphism with congenital anorectal malformation and Hirschsprung disease.
Hong GAO ; Dajia WANG ; Xiangxuan ZHAO ; Jie MI ; Yuzuo BAI ; Weilin WANG
Chinese Journal of Gastrointestinal Surgery 2015;18(7):707-712
OBJECTIVETo explore the relationship of Ghrelin gene polymorphism with the occurrence of human anorectal malformations (ARMs) and Hirschsprung disease(HSCR).
METHODSPCR and DNA sequencing were used to detect the single nucleotide polymorphism (SNPs) of 3 loci (rs139684563, rs149447194, rs186599567) genotype of Ghrelin gene in 100 children with ARMs, 100 children with HSCR, and 100 healthy children (normal group). Genovariation and gene mutation were analyzed with case-control method.
RESULTSThree loci SNPs were in accordance with Hardy-Weinberg genetic equilibrium. No significant differences were found in rs139684563 allele and genotype frequencies between the cases and the normal groups (P>0.05). The allele and genotype frequencies of rs149447194 and rs186599567 were significantly different between cases and normal group (P<0.05). DNA sequencing results showed that wild-type homozygous deletion (176th and 191th base A deletion, respectively) were found in rs149447194 and rs186599567of ARMs and HSCR children, and single base substitution was detected in rs149447194 of ARMs children (194th codon nucleotide CCT to CTC).
CONCLUSIONSThe rs149447194 and the rs186599567 polymorphism changes may be associated with the pathogenesis of ARMs and HSCR.
Alleles ; Base Sequence ; Gene Frequency ; Genotype ; Ghrelin ; Hirschsprung Disease ; Humans ; Mutation ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide ; Rectal Diseases ; Sequence Analysis, DNA
5.Clinical features and genetic analysis of a fetus with holoprosencephaly.
Jinzhe YU ; Chuang LI ; Yan ZHANG ; Jesse LI-LING ; Yuan LYU ; Hong CUI
Chinese Journal of Medical Genetics 2020;37(5):547-550
OBJECTIVE:
To analyze the clinical features and pathogenesis of a fetus with holoprosencephaly.
METHODS:
The findings of prenatal ultrasonography was reviewed. Following elective abortion, whole exome sequencing (WES) was carried out to identify potential pathogenic variant. Copy number variants (CNVs) of the abortus and its parents were detected by low-depth high-throughput sequencing. The parents were also analyzed by chromosomal karyotyping.
RESULTS:
Prenatal ultrasound suggested that the fetus had holoprosencephaly. WES revealed that it had approximately 33 Mb deletion at chromosome 13 involving ZIC2, a haploid dose sensitive gene. The results of low-depth high-throughput sequencing confirmed that the fetus carried a de novo 32.32 Mb deletion at 13q31.1-34. Karyotyping analysis has excluded gross chromosomal aberration in both parents.
CONCLUSION
The fetus was diagnosed with holoprosencephaly, which may be attributable to the 13q31.1-34 deletion involving the ZIC2 gene.
Adult
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Chromosomes, Human, Pair 13
;
genetics
;
Female
;
Fetus
;
Genetic Testing
;
Holoprosencephaly
;
diagnostic imaging
;
genetics
;
pathology
;
Humans
;
Karyotyping
;
Male
;
Nuclear Proteins
;
genetics
;
Pregnancy
;
Prenatal Diagnosis
;
Sequence Deletion
;
Transcription Factors
;
genetics
;
Ultrasonography, Prenatal
;
Whole Exome Sequencing
6.Clinical and genetic analysis of a child with mental retardation and microcephaly with pontine and cerebellar hypoplasia.
Ziwei WANG ; Chuang LI ; Yan ZHAO ; Ling LI ; Yuan LYU ; Hong CUI
Chinese Journal of Medical Genetics 2021;38(10):985-988
OBJECTIVE:
To analyze the clinical phenotype and pathogenic variant in a child diagnosed with mental retardation and microcephaly with pontine and cerebellar hypoplasia (MICPCH).
METHODS:
Clinical phenotype of the child was reviewed. Whole exome sequencing was carried out for the child. Candidate variant was verified by Sanger sequencing of the family member.
RESULTS:
The proband manifested dyskinesia, development delay, cerebellar hypoplasia and bilateral hearing impairment. WES results revealed that the proband has carried a pathogenic c.1641_1644delACAA (p.Thr548Trpfs*69) variant of the CASK gene, which was verified by Sanger sequencing to be a de novo variant.
CONCLUSION
The c.1641_1644delACAA (p.Thr548Trpfs*69) variant of the CASK gene probably underlay the MICPCH in the proband. Above finding has provided a basis for genetic counseling. WES should be considered for the diagnosis of neurological dysplasia.
Cerebellum/abnormalities*
;
Child
;
Developmental Disabilities
;
Family
;
Humans
;
Mental Retardation, X-Linked
;
Microcephaly/genetics*
;
Nervous System Malformations
7.CAR Technology and Its Application in Treatment of Multiple Myeloma--Review.
Tong LI ; Hong-Tao WANG ; Zhuo-Gang LIU
Journal of Experimental Hematology 2016;24(1):279-284
Multiple myeloma (MM) is a hematologic malignancy resulted from genetic mutations in the process of B lymphocyte differentiating into plasma cells, the chemotherapy is the main treatment method, especially with the development of proteasome inhibitors and other drugs, the overall survival rate of MM patients has improved greatly, but the chemoresistance is still an important reason for treatment failure. Chimeric antigen receptor (CAR)-modified T lymphocyte therapy is a new method for tumor adoptive immunotherapy. By means of genetic modification, T cells are able to identify the target antigen specifically, and to kill target cells without major histocompatibility complex (MHC) restriction, therefore the specific killing activity is conspicuous, which has got considerable attention by the public, and has made remarkable achievements particularly in the treatment of B-lineage leukemia and lymphoma, but no systematic literatures were reported in the field of multiple myeloma using CAR therapy. Therefore, this review summarizes the research results of different CAR target in vivo and in vitro experiments for multiple myeloma.
Genetic Therapy
;
Humans
;
Immunotherapy, Adoptive
;
methods
;
Multiple Myeloma
;
therapy
;
Receptors, Antigen, T-Cell
;
T-Lymphocytes
;
cytology
8.Prognostic Significance of CD27 and CD56 on Newly Diagnosed MM Patients Treated with Bortezomib.
Cai-Xia ZHANG ; Hong-Tao WANG ; Guo-Jun ZHANG
Journal of Experimental Hematology 2021;29(3):827-831
OBJECTIVE:
To investigate the significance of CD27 and CD56 in the prognosis of multiple myeloma (MM) patients, and to establish a simple and convenient prognostic risk score.
METHODS:
One hundred and eleven newly diagnosed MM patients treated by bortezomib in Shengjing hospital from January 1, 2013 to January 1, 2019 were selected, and the relationship between clinical characteristics and survival time of patients was analyzed.
RESULTS:
The overall survival (OS) of patients in CD27
CONCLUSION
Among patients with MM treated by bortezomib, CD27
Bortezomib
;
CD56 Antigen
;
Hematopoietic Stem Cell Transplantation
;
Humans
;
Multiple Myeloma
;
Prognosis
;
Retrospective Studies
9.Branchial Anomalies in Korea: A Survey by the Korean Association of Pediatric Surgeons
WH PARK ; SI KWON ; SY KIM ; SC KIM ; SK KIM ; WK KIM ; IK KIM ; JE KIM ; HH KIM ; KW PARK ; YS PARK ; JS PARK ; YT SONG ; WS AHN ; NK OH ; SM OH ; SY YOO ; NH LEE ; OS LEE ; MD LEE ; SC LEE ; SK LEE ; SI CHANG ; YS CHUN ; ES CHUNG ; SY CHUNG ; SE CHUNG ; PM CHUNG ; MH CHO ; KJ CHOI ; SO CHOI ; SH CHOI ; SJ HAN ; YS HUH ; C HONG ; EH WHANG
Journal of the Korean Association of Pediatric Surgeons 1996;2(2):119-128
The survey on branchial anomalies was conducted by Korean Association of Pediatric Surgeons. A total of 173 cases were reported, which were managed by 36 members and cooperators during the three years from January 1, 1993 through December 31, 1995. The following results were obtained by retrospective analysis of the 173 cases of branchial anomalies. The presenting symptoms were cervical mass in 101 cases, pit with or without discharge in 71, cervical abscess in 47 and respiratory difficulty in 3. The average age of the patients with cervical abscess was 52 months. Seventy(79%) of 89 patients with branchial anomalies and a cystic mass had their first clinical manifestations by 1 year of age, while 40(51%) of 78 patients with only a branchial cyst had their first clinical manifestation in first year of life. Radiologic studies were carried out in 77 patients(43%). The preferred diagnostic modalities were ultrasonography(47 patients), simple neck radiogram(l9) and CT scan(17). Preoperative diagnosis was correctly made in 156(91%) of 173 patients. Seventeen patients were incorrectly diagnosed as thyroglossal duct cyst in 5 patients, cystic hygroma in 4, dermoid cyst in 3, and lymphadenopathy in 3. There were no remarkable difference in sex and laterality of presentation but bilateral lesions were found in 9(5%) patients and unusual locations of the anomalies were the manubrium, left subclavicular area, median cervial area, preauricular and parotid area. There were 78(45%) patients with cyst, 52(30%) patients with sinus, 35(20%) patients with fistula and 8(5%) patient with skin tag. Embryological classification was possible in only 64(37%) patients. The 2nd branchial anomaly was present in 50 (78%), the 1st branchial anomaly in 10(18%), and the 3rd or 4th branchial anomaly in 4(6%). Histopathological study of the lining epithelium(N=134) is recorded that 45% were lined with squamous epithelium, 17% with respiratory epithelium, 6% with. squamous and respiratory epithelium, 14% with inflammatory change. Lymphoid tissue was common(62%) in the wall of the lesions. Twelve(7%) of 158 patients had postoperative complications including wound complication, recurrence and facial nerve palsy.
Abscess
;
Branchioma
;
Child
;
Classification
;
Dermoid Cyst
;
Diagnosis
;
Epithelium
;
Facial Nerve
;
Fistula
;
Humans
;
Korea
;
Lymphangioma, Cystic
;
Lymphatic Diseases
;
Lymphoid Tissue
;
Manubrium
;
Neck
;
Paralysis
;
Postoperative Complications
;
Recurrence
;
Respiratory Mucosa
;
Retrospective Studies
;
Skin
;
Surgeons
;
Thyroglossal Cyst
;
Wounds and Injuries
10.Effects of EGCG on Proliferation, Cell Cycle and DAPK1 Gene Methylation of Acute Promyelocytic Leukemia NB4 Cell Line.
Xu SHI ; Hong-Yu GAO ; Wei YAN ; Xiao-Wei HE ; We YANG
Journal of Experimental Hematology 2018;26(5):1288-1293
OBJECTIVETo investigate the effects of epigallocatechin-3-gallate (EGCG) on proliferation and cell cycle of acute promyelocytic leukemia NB4 cell line and to clarify the molecular mechanism.
METHODSNB4 cells were treated with 0,50,75,100 and 125µmol/L of EGCG for 24, 48, 72 and 96 h, respectively. The proliferation level of NB4 cells was measured by CCK-8 assay. The cell cycle progression of NB4 cells was assayed by flow cytometry. The mRNA expression levels of DNMT1, DNMT3a and DAPK1 were detected by RT-PCR. The methylation status of gene was tested by methylation specific PCR, and the expression level of DAPK1 protein was detected by Western blot.
RESULTSThe proliferation and cell cycle progression of NB4 cells treated with EGCG were inhibited and showed the characteristic of time-dependent and dose-dependent manner. The expression level of DAPK1 and DNMT3a decreased in NB4 cells treated with EGCG. The expression level of DAPK increased in NB4 cells treated with EGCG, while the methylation of DAPK1 gene decreased.
CONCLUSIONEGCG inhibits the proliferation and cell cycle progression of NB4 cells by inhibiting the expression of DNMT1 and DNMT3a and down-regulating the methylation status of DAPK1 gene.