No abstract available.
Animals ; DNA* ; LLC-PK1 Cells* ; Swine

The purpose of this study was to evaluate and compare the fit of denture bases processed by injection pressing technic using laser scanner of reverse engineering technic. The auther duplicated 20 maxillary edentulous models and 20 mandibular edentulous models, which were scanned on HYSCAN 45C 3D SCANNER(Hymarc Co., Canada). The scanned data was stored in the personal computer using SURFACER (Imageware Co., U.S.A.) software program. After 40 dentures were cured by PERform Inkovac system. SR-Ivocap system, Palajet system, and Sulfon system, they were stored in water at room temperature for 24 hours. The dentures were scanned on HYSCAN 45C 3D SCANNER(Hymarc Co. Canada). The scanned data were stored in the personal computer using SURFACER (Imageware Co., U.S.A) software program. By overlapping two images using the same program, the fit between two surfaces was scaled by positive and negative errors. The obtained results were as follows : 1. In the upper denture, most of the positive errors occurred on the lingual side of anterior alveolar ridge and the negative errors were on the flange of denture bases. 2. In the lower denture, most of the positive errors occurred on the inner side of lingual flange and the negative errors were on the border of anterior labial flange areas. 3. There were no statistical differences among the positive errors of the four types of inject-ion denture curing methods and also no statistical differences between negative errors except only in negative maximum errors. 4. In PRERform system and SR-Ivocap system, they have the tendency of inaccurate fit lower denture bases comparing to that of upper denture bases. 5. The negative error scales were greater than the positive error scales in all types of inject-ion denture curing methods.
Alveolar Process ; Denture Bases* ; Dentures* ; Microcomputers ; Water ; Weights and Measures

Objective The paper analyzes the operating patterns of the outpatient process and identifies the links needing reengineering so as to provide scientific basis for the optimal allocation of outpatient resources and process reengineering and prove the rationality and feasibility of using the queuing theory. Methods Using the methods of the queuing theory, the service time and the patients' arrival time at such service links as registration, billing and accounting, internal medicine, and gynecology in the outpatient department of a certain hospital were measured and the operational indexes at various links such as service intensity, average queuing length, average queuing time, average stay, probability of the service desks being idle, and the probability of the patients having to wait were calculated so as to estimate the rational number of service desks and the optimal value of the sum of the cost of waiting and the cost of service. Results Registration, billing and accounting , service intensity in internal medicine, personnel allocation, and queuing time were basically rational in the outpatient department of the hospital surveyed. One more physician should be added to the gynecology department so as to improve efficiency and reduce patients' waiting time and queuing length. There was currently a shortage of medical staff in the hospital. The cost of hospital input was moderate while the cost of waiting was on the high side. Conclusion It is rational and feasible to evaluate the efficiency of the outpatient process with the methods of the queuing theory. The method deserves to be spread.

Objective Detection of S-100B protein in different degree meconium pollution level in serum of children with value.MethodsIn 2012 June to 2013 December in our department were simple meconium stained amniotic fluid in term newborns in 73 cases, and set up for the observation group, and according to the amniotic fluid pollution degree is divided into 47 cases of amniotic fluid of Ⅰ-Ⅱdegree pollution group and 26 cases in grade Ⅲmeconium group during the same period, selected 20 cases without amniotic fluid contamination in term healthy newborns for the control group, the groups were compared in S-100B protein content difference.ResultsⅢmeconium stained amniotic fluid were within 6h serum S-100B was significantly higher than that in control group(P<0.01), and the degree of contamination of amniotic fluidⅠ-Ⅱgroup compared with the control group, no significant difference(P>0.05).Ⅲ meconium stained amniotic fluid in children with 72h also increased.ConclusionThird degree meconium stained amniotic fluid but normal Apgar score of newborns may still exist in clinical brain injury, so pay close attention to.

Objective:To identify the function of extracellular soluble vimentin that promotes proliferation, activation and chemotaxis of inflammatory cells. Methods: The proliferation of rat splenocytes stimulated with vimentin was evaluated in vitro. The lymphocyte counts and vimentin-antibody levels of peripheral blood in mice immunized with vimentin were detected in vivo. Peritoneal macrophages were collected and cultured with different concentrations of vimentin to detect the effect on phagocytosing chicken red blood cells. The chemotaxis of NIH3T3 fibroblast towards vimentin was observed in transwell chamber. Results:In vitro vimentin dose dependently promoted the proliferation of splenocytes. The proliferation indexes of primed and naive splenocytes cultured with 16μg/ml vimentin were reached up to ( 196. 0 ± 9. 7 )% and ( 208. 9 ± 4. 6 )% respectively without significant difference. In vivo vimentin significantly enhanced the lymphocytes number(109 L-1)of peripheral blood(5. 74±0. 51 vs. 1. 69±0. 13)and the levels of vimentin specific antibody( OD value 2. 31 ± 0. 06 vs. 0. 19 ± 0. 08 ) that shown no significant difference from immunization with vimentin plus CFA. In vitro vimentin dose dependently stimulated phagocytic ability of macrophages and performed the chemotactic effects on NIH3T3 fibroblasts. Conclusion:Extracellular soluble vimentin promotes the proliferation,activation and chemotaxis of concerned inflammatory cells and possesses the functions as an alarmin.

Accidents, Occupational ; Adolescent ; Carbon Monoxide Poisoning ; Humans ; Male ; Young Adult

PURPOSE: To investigate the effect of triamcinolone acetonide (TA) on the survival, nitric oxide (NO) production, and migration of cultured human trabecular meshwork (TM) cells. METHODS: After exposure to TA, indomethacin, and dexamethasone for 2 days, the survival and nitrite production of the primarily cultured human TM cells were assessed with MTT and Griess assays respectively. The effect of co-exposure to the NO donor, sodium nitroprusside, was also assessed. A migration assay was done to evaluate the effect of TA on the activity of TM cells. RESULTS: Cellular survival increased after exposure to TA at low concentration but decreased at high concentration. TA decreased the production of NO significantly (p<0.05). Exposure to indomethacin and dexamethasone revealed similar results. TA reversibly inhibited the migration of TM cells. CONCLUSIONS: TA decreases the production of NO and inhibits the migration of TM cells. TA at high concentration decreases cellular survival accompanied with decreased NO production. These effects of TA on TM cells may result in the elevation of intraocular pressure.
Dexamethasone ; Humans ; Indomethacin ; Intraocular Pressure ; Nitric Oxide ; Nitroprusside ; Tissue Donors ; Trabecular Meshwork* ; Triamcinolone Acetonide* ; Triamcinolone*

PURPOSE: To report a case of fungal keratitis 3 days after intracorneal ring segment (ICRS) implantation for keratoconus. CASE SUMMARY: A 65-year-old woman was referred to our clinic with refractory infectious keratitis in her left eye 3 days after ICRS insertion for keratoconus. Slit lamp examinations revealed infiltrates around the incision site with cellular reaction in the anterior chamber after the ICRS had been removed. Corneal scrapings were obtained for staining and cultures, and intensive topical antibiotics were administered. Initial microscopy and cultures were negative. Despite the use of intensive topical antibiotics, there was no improvement. Hyphae were isolated from additional corneal scrapings. The patient's symptoms and corneal findings improved following administration of topical amphotericin B and oral itraconazole. CONCLUSIONS: Infectious keratitis after ICRS implantation is an uncommon but sight-threatening complication. Fungal keratitis should also be considered if infectious keratitis after ICRS is unresponsive to antibiotics.
Aged ; Amphotericin B ; Anterior Chamber ; Anti-Bacterial Agents ; Eye ; Female ; Humans ; Hyphae ; Keratitis ; Keratoconus ; Microscopy

BACKGROUND: It seems that herpes zoster is caused by reactivation of the varicella-zoster virus and its incidence is increasing. The reactivation of the varicella zoster virus is thought to be associated with the disturbance of the state of immunity in patients with herpes zoster. OBJECTIVE: The purpose of this study was to elucidate the state of immunity in patients with herpes zoster in its acute phase(less than 7 days). METHODS: 1. Thirty patients with acute phase herpes zoster matched by age and sex against a control group, were checked for Helper/Inducer T cell(CD4), Suppressor/Cytotoxic T cell(CD8), NK cell, B cell and activated T cell by three color flow cytometric analysis. 2. Forty patients with herpes zoster measured delayed cutaneous hypersensitivity by means of Multitest' CMI. 3. Thirty patients with herpes zoster measured Ig G, M, A by means of N-antisera method.
Herpes Zoster* ; Herpesvirus 3, Human ; Humans ; Hypersensitivity ; Immunity, Cellular ; Immunity, Humoral* ; Incidence ; Killer Cells, Natural

In case of limbitis, cytokines secreted by PMNs might inhibit the function of corneal epithelial stem cells located at the limbal basal cell layer. The purpose of this study was to examine whether PMNs factor affected stem cells (SC). PMNs obtained from the peritoneal cavity of rabbits after 0.2% glycogen stimulation incubated in DMEM at 37 degrees C for 24 hours. PMNs factor was obtained from the medium using dialysis. The death or inhibition of growth of rabbit corneal epithelial cells (CE) by PMNs factor was studied in vitro. PMNs factor and PBS as a control were injected into the limbal region for clinical evaluation and histopathologic study. ID 50 of PMNs factor for cultured CE was equal to the amount of polypeptide produced by 1.29 x 0 (7) PMNs. Cultured rabbit CE shrank and began to die 24 hours after exposure to PMNs factor. Abnormal findings were observed 5 days and 8 months after injection of PMNs factor at the limbus by light and electron microscopy. Clouding, defect, and vascularization of CE were noted several months after injection of PMNs factor. PMNs in limbitis may damage corneal epithelial stem cells and cause epithelial instability of the cornea.
Cornea ; Cytokines ; Dialysis ; Epithelial Cells ; Glycogen ; Microscopy, Electron ; Neutrophils* ; Peritoneal Cavity ; Rabbits ; Stem Cells*