Stromal cell-derived factor-1(SDF-1)is an early cell growth factor which belongs to a subfamily of chemokines.The CXCR4 is SDF-1 receptor.The SDF-1 interacting with CXCR4 plays important roles in physiological and pathological processes such as hematopiesis, stem cell homing,angiogenesis and leukemic cells infiltrating.To study the expression of CXCR4 in leukemia and the relationship between CXCR4 and leukemic cells infiltration can add indicators for leukemic MIC diagnosis of immunology indexes and have great significance to take molecule targeted therapy for preventing leukemia from infiltration or relapse as well.

Tuo-Fawas one of three internal treatment rules in traditional Chinese medicine (TCM) surgery. It had been widely used in the treatment of superficial suppurative diseases, visceral suppurative diseases and other diseases. This article was aimed to clarify the action mechanism ofTuo-Fa for carrying forward better services in the clinic. This article reviewed and recapitulated related literatures on experimental researches ofTuo-Fa in recent years. It mainly showed the research status on anti-infection, anti-tumor and anti-gastric ulcer withTuo-Fa and its prescriptions. It summarized the existed shortcomings, which was that experimental researches on the action mechanism ofTuo-Fa was still on the initial stage with a few references and obviously lagged behind clinical applications. However, some achievements were also made. It had initially revealed the action mechanism ofTuo-Fa in treatment of pyogenic infection. It also had research on action mechanism of anti-tumor and anti-gastric ulcer treatment. It developed new field for the clinical practice. Finally, the research on action mechanism ofTuo-Fa and future prospects were expressed.

In this paper, the authors analyzed the current problems in domestic hospital nursing human re-source such as widespread erosion, nursing staff slack and others. From the characteristics of nursing ethics and be-havior dynamic analysis, suggest to establish a focus on the humanities concern nursing ethics culture of the people-oriented, fundamentally effectively motivate nurses, specific include: respecting nurses and ethical personality differences;to establish a trust mechanism for nursing staff;meet the needs of nursing staff learning;to establish a nursing staff self management team, thus improve hospital nursing ethics management work, for the nursing staff to provide a stable and efficient working and living environment.

Objective To explore the mechanism of the microglia inflammatory response through observing the changes of interleukin-1 receptor associated kinase-4(IRAK-4) expression in murine N9 microglia cells under hypoxia.Methods N9 cells were exposed to 3%O2,5%CO2,92%N2 for 1,3,6,12 and 24 h respectively.The IRAK-4 protein level was detected by Western blotting and the celluar change was observed by laser scanning confocal microscope(LSCM).The TNF-? level was measured by ELISA.Results After hypoxia,the expression of IRAK-4 protein was increased in a time-dependent manner.The elevation began at 1 h after hypoxia,increased significantly at 3 h(P0.05).The fluorescence intensity of IRAK-4 also increased with the extension of anoxic time.The TNF-? level change were similar.There were significant positive correlations between the expression of IRAK-4 and TNF-?(P

Objective To investigate the clinical features,causes,pathogenesis and treatment of acute pancreatitis in patients with systemic lupus erythematosus (SLE).Methods From January 1983 to March 2003,15 SLE patients with acute pancreatitis were retrospectively analyzed.Results There were 12 patients with mild acute pancreatitis and 3 patients with severe acute pancreatitis.All patients received the treatment of moderate to large dosage of corticosteroids.Five patients recovered.Five patients improved.Five patients died.Conclusion (1)Acute pancreatitis occurs in the active stage of SLE.(2)The pancreatic vascular lesion is the main pathogenic mechanism in SLE acute pancreatitis.(3)The corticosteroids may not be the cause of SLE pancreastitis.(4)The treatment with moderate to high doses of corticosteroids is safe and effective.

Objective To investigate the relevant factors of hyperthyroidism complicating hyperthyroid heart disease.Method Among 48 cases,those factors including age,course of disease,condition and regular treatment were analyzed with Logistic multiple factor reg-ression analysis.Result The hyperthyroid heart disases was related with age,course of disease,condition and whether to treat regularly.Conclusions There are many factors to lead to hyperthroidism complicating hyperthyroid heart disease.It should be paid attention to in those patients with elder,longer course of disease,serious condition and no regular treatment.

Experimental respiratory distress syndrome was induced with oleic acid injection to 8 dogs. 4 dogs received no treatment and served as controls, and the other 4 were treated with anisodamine (654-2) after oleic acid injection.All the animals were killed at the 72nd hour after injection, and their lung tissues were studied under electron microscope.It was found that anisodamine was able to reduce the severity of pulmonary edema. There was accumulation of neutrophils in the pulmonary capillaries and hyaline membrane formation on the epithelial surface of the alveoli and alveolar duct walls. Neutrophil accumulation in the capillaries may indicate that the content of the blood was kept at a fairly high level at the 72nd hour after injection in spite of anisodamine treatment. The chief constituent of the hyaline membrane was fibrin. At the sites of hyaline membrane formation, focal cyto-plasmic necrosis of the type I epithelial cells was often observed.

Objective To observe the relationship among follicular development and insulin-like growth factor Ⅰ (IGF-Ⅰ) and IGF-Ⅰ receptor (IGF-ⅠR) mRNA during ovulation stimulation period in an in-vitro fertilization (IVF) program. Methods IGF-Ⅰ levels in serum samples obtained before (menstrual day 3) and after stimulated (at the time of ovum pick-up) and follicular fluid samples were measured by ELISA. The expression of IGF-Ⅰ mRNA in the granulosa cell obtained from follicular aspiration was determined by RT-PCR. Results There was no significant difference between IGF-Ⅰ levels of serum samples before or after stimulated and follicular fluid. In patients with less than 4 follicls (diameter≥14 mm) at the time of ovum pick-up the IGF-Ⅰ mRNA level was significantly lower than that in patients with more than 4 follicles. Conclusion The serum IGF-Ⅰ levels fail to predict the numbers of follicular development; the levels of IGF-ⅠR mRNA in granulosa cells forecast the responsiveness of ovary to the stimulation of gonadotropins in an IVF program.

Forty rats were selected for the experiment, which were divided into three groups including experimental ulcer group, normal saline and normal control groups. The pancreatic tissue were prepared for immunohistochemical staining at 6th, 10th, 14th, and 21st day after operation. Sternberger's PAP method was used to show the glucagon cell (A cell) and insulin cell (B cell) in order to observe their changes during the experimental peptic ulcer. In normal control group, the percentage of A cells is 23.31?1.91 (X?SD) and that of B cells is 73.1?4.01. But in experimental ulcer group, the A cell percentage is raised up to 42.6?5.59 and the B cell percentage is lowered down to 57.5?4.55 on the 10th day after operation. Comparing with normal saline group and normal group their differences show statistical outstanding differences. The result may point out that both A and B cells of pancreatic islet take part in the body's natural resistance against the disease, especially A cells.

Objective To investigate the effects of insulin-like growth factor Ⅰ (IGF-Ⅰ) and insulin-like growth factor binding-protein 1 (IGFBP-1) on development of ovarian follicles during gonadotropin stimulation in in-vitro fertilization (IVF) program. Methods Enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay were used to determine the levels of IGF-Ⅰ, IGFBP-1 and estradiol (E 2) in serum and follicular fluid obtained during oocyte pick-up (OPU) in 32 IVF-embryo transfer cycles. Results (1) Serum IGFBP-1 levels increased with growth of follicles [(3 5?1 0) ?g/L during OPU, (2 1?0 5) ?g/L before stimulation, P 0 05]. Significant positive correlation was found between serum IGFBP-1 and serum E 2 [(3 5?1 0) ?g/L, (3 293? 1 361) pmol/L, r =0 41, P