1.The mechanism of invariant natural killer T cells in regulating autoimmune diseases
Chinese Journal of Experimental Ophthalmology 2013;31(12):1183-1186
Studies demonstrated that natural killer T (NKT) cells are involved in the diverse immunomodulatory responses.But the mechanism that NKT cells regulate pathogenic T helper cells (Th) are still unclear.Recent researches found that Th17 cells are associated with a variety of autoimmune disease and play a crucial role in autoimmune diseases,which is very different from previous thesis that Th1 and Th2 are the key factors in the immune response in the patients with autoimmune disease.Studies both in vitro and in vivo also documented that invariant NKT cells (iNKT) inhibit the differentiation of CD4+ T cells toward Th1 and Th17 cells at the different mechanisms.The concept of NKT cells,the regulation of NKT cells to Th1 and Th17 cells in the autoimmune diseases were reviewed.
2.Anatomical study and special guiding devices design for the operation of upper cervical fixation with Atlas titanium cable
Chinese Journal of Primary Medicine and Pharmacy 2005;0(11):-
Objective To observe morphological character of posterior arch of Atlas and lamina of axis,as well as provide anatomical basis to design special guiding device for the operation of upper cervical posterior fixation with Atlas titanium cable so as to make the operation safe and easy.Methods The posterior arch of Atlas and lami- na of axis were observed and measured in 100 adult dry Atlas and axis specimens,then special guiding devices were designed based on the anatomical data.Results (1)The posterior arch of Atlas was thin with a fan-shape section, whose inner plane descended from back to inside and the lamina of axis was stronger and thicker than posterior arch of Atlas,with a vertical inner plane and a ridge near the lower edge.(2)The special guiding device for posterior arch of Atlas should be designed with a bend head as well that for lamina of axis should be designed with a round head. They were used in 61 operations which became safer and easier.Conclusion The operation of upper cervical posteri- or fixation with Atlas titanium cable would be safer with the help of special guiding devices which were designed based on the anatomical character.
3.Age-Dependent Feature of Damage of Hippocampus at Different Maturational Stages after Repeated Seizures in Rats
jia-sheng, HU ; zhi-sheng, LIU ; ya-ling, HUANG
Journal of Applied Clinical Pediatrics 2004;0(12):-
Objective To observe age-dependent feature of damage of hippocampus to different maturational stages rats after kindling repeated seizures.Methods The effects of 5 daily pentylenetetrazol-induced convulsions in different rats beginning at postnatal day 10,20,60(P10,P20,P60)were evaluated.In the 3 groups,Thionin staining method was utilized to observe morphological changes and cell counting of dentate granule cells,CA3,CA1,and hilar neurons.Timm's method of silver sulfide staining was adopted to observe the mossy fiber sprouting.Results 1.Cell counting of CA1,CA3 and hilar neurons in P10 and P20 groups demonstrated no differences from controls in rats,whereas P60 with daily seizures had a significant decrease in CA1,CA3 neurons(8.22?1.88,5.62?1.68 vs 6.31?1.50,3.62?1.40)(t=2.246,2.587 Pa
4.Progress in the research on commonly used anti-cancer traditional Chinese medicine capsules combined with chemotherapy on middle-advanced stage lung cancer.
Li BIAN ; Si-sheng TIAN ; Ya-lin CHEN
Chinese Journal of Integrated Traditional and Western Medicine 2009;29(3):279-282
Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Capsules
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Carcinoma, Non-Small-Cell Lung
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drug therapy
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Drug Therapy, Combination
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Drugs, Chinese Herbal
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therapeutic use
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Humans
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Integrative Medicine
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Lung Neoplasms
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drug therapy
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Phytotherapy
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Small Cell Lung Carcinoma
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drug therapy
5.Analysis of the Changes of Burden Factor Loading for Chronic Disease Inpatients before and after the Im-plementation of Zero Price Policy by Establishing Structure Equation Model
Xiaoyan PAN ; Ya NIU ; Sheng TIAN ; Shanshan TONG
China Pharmacy 2017;28(11):1452-1455
OBJECTIVE:To construct the structure equation model for the burden of chronic disease inpatients in primary hos-pital before and after the implementation of zero price policy,and analyze the changes of burden factor loading. METHODS:6 pri-mary hospitals were randomly selected in Danyang,Jiangsu province. The data of hospitalization expenses for chronic diseases(hy-pertension,diabetes,bronchitis)were collected before and after the implementation of zero price policy. Using drug cost,nursing fees,inspection fees and treatment fees as independent variables,hospitalization burden as latent variable,SPSS and AMOS 24.0 software were adopted to establish the model. RESULTS:The burden factor loading of inpatients changed greatly before and after the implementation of zero price policy. χ2=24.586,χ2/df=1.446, RMSEA=0.019,GFI=0.995,AGFI=0.989,CFI=0.988,NFI=0.963 manifested good model fitting. Factor loading of drug cost increased greatly,indicating the burden of inpatients was reduced after the implementation of zero price policy. Factor loading of inspection fees and treatment fees increased significantly,the charac-terization effects of them to the burden of inpatients were enhanced. Factor loading of nursing fees was the lowest,and it had the weakest effects on the burden of inpatients,of which the government should enhance the regulation. CONCLUSIONS:Established model has good reliability and validity. It can reflect the change of burden factor loading of inpatients before and after the implemen-tation of zero price policy.
6. Comparative study on GC-MS fingerprints of volatile oil in crude and processed Cyperi Rhizoma
Chinese Traditional and Herbal Drugs 2013;44(23):3321-3327
Objective: To establish the GC-MS fingerprints of volatile oil in crude, water-processed, and vinegar-processed Cyperi Rhizoma, and to capture the whole scope of the chemical constituents changing. Methods: The fingerprints of volatile oil in crude, water-processed, and vinegar-processed Cyperi Rhizoma were established by GC-MS. And the data were analyzed by chromatographic fingerprint similarity evaluation software for Chinese materia medica (Version A). Results: The similarities of each group were good, which indicated that the technology was stable and feasible. The crude group gained 52 common peaks, 28 as the water-processed group, and 53 as the vinegar-processed group. Meanwhile, 46 chemical components among the peaks above were identified in total. Comparing with the three groups, the kinds and contents of many components in each group were changed after processing. With the crude group as reference, the differences between the water-processed group and vinegar-processed group were contrastively analyzed before and after processing, and the Cyperi Rhizoma processed with vinegar could remain the chemical constitutions in its volatile oil better. Conclusion: Using vinegar as processing accessory is scientific and reasonable. Vinegar could play a role in protecting the compounds of volatile oil in Cyperi Rhizoma steadily.
8.Production and Preliminary Application of Monoclonal Antibodies Raised Against Aβ22-35
Jing-wei, SHENG ; Ya-er, HU ; Zong-qin, XIA
Journal of Shanghai Jiaotong University(Medical Science) 2001;21(2):110-112,140
Objective To produce a hybridoma secreting stable monoclonal antibodies against Aβ22-35 and to develop a detection method for the assay of Aβ. Methods Spleen cells from Balb/cmice immunized with Aβ22-35-KLH were fused with mouse myeloma cells SP2/0. The techniques of immunoprecipitation and western blotting plus ECL were used to investigate the levels of Aβ in the rat brain. Results Two strains of hybridomas (3A8 and 3B2) secreting stable monoclonal antibodies raised against Aβ22-35 were obtained. The subtypes of Aβ22-35 were IgG3. The levels of Aβ in young and older rat brain were 9.8±2.8 and 13.36±2.65 (pmol/12mg brain tissues, x±s), respectively. Conclusion The Aβ22-35 mAb obtained had high titres and specificity. The levels of Aβ in the older rat brain were significantly increased as compared with the young one (P<0.05).
9.Usage of renoportal anastomosis in the liver transplantation
Zhi-Jun ZHU ; Ming-Sheng HUAI ; Ya-Min ZHANG ;
Chinese Journal of Organ Transplantation 2003;0(06):-
Objective To evaluate the usage of renoportal anastomosis in the liver transplanta- tion.Method The successful experience about renoportal anastomosis for portal vein reconstruction in liver transplantation was reported,and related literature reviewed.Results Including our case,there have been 13 cases of liver transplantation using renoportal anastomosis for portal vein reconstruction. Among these patients,8 cases were complicated with diffuse portal vein thrombosis,and 10 cases had a splenorenal shunt(spontaneous shunt in 3 and surgical shunt in 7).Complications related to portal vein hypertension occurred in 3 cases(transient ascites in 2 cases and severe digestive bleeding in 1 case)after liver transplantation.There were 3 deaths which were not related to renoportal anastomo- sis.Conclusion Renoportal anastomosis is a safe and feasible technique in liver transplantation for the patients with diffuse portal vein thrombosis or with splenorenal shunt(spontaneous or surgical shunt).
10.The mechanism of inhibitory control in sensation seeking:an event-related potential study
Wenbin SHENG ; Jing XU ; Ya ZHENG ; Fei TAN ; Huijuan SHEN
Chinese Journal of Behavioral Medicine and Brain Science 2011;20(1):25-27
Objective To investigate the neural mechanism of inhibitory control in sensation seeking by using the event-related potential(ERP) technique. Methods High and low sensation seekers( 16 people in each group ), who were selected according to their sensation seeking scores, performed a Go/Nogo task in which the stimuli possessed two levels of difficulty. Electro- encephalogram(EEG) signals were recorded continuously by a set of 32 Ag/AgCI electrodes. Results For two types of stimuli ( Congruent, Incongruent) :( 1 )The amplitude(FCZ) of Nogo N2 and Nogo P3 were( (1.61 ±4.25)μV,(-2.32±4.55)μV)and((16.44±5.74)μV,(17.00±5.71)μV). (2)There was no significant main effects of group for the Nogo N2 amplitude( F (1.30) =0.31, P=0. 59,η2=0. 01;F(1.30) =0.07,P=0.80,η2=0.002) ,the N2d amplitude( F(1.30) =1.18,P=0.29,η2=0.04;F(1.30) =0.004, P=0.95, η2 < 0.001 ) ,the Nogo P3 amplitude( F (1.30) =0.13, P=0.72, η2 =0.004;F(1.30)=0.28, P=0.60, η2 =0.009) and the P3d amplitude( F(1.30) =0.08, P=0.50, η2 =0.02; F (1.30) =0.56,P=0.46, η2 =0.02). (3)Neither of main effects for the N2 and P3 latency was significant (P>0.05). Conclusion The inhibitory control is similar across high and low sensation seeking groups,indicating that there is no relationship between the sensation seeking behaviors and the individual inhibitory control.