Fifteen cases of chronic pelvic inflammation were treated by needling Guanyuan (CV 4), Qihai (CV 6), Sanyinjiao (SP 6), Zhongji (CV 3), Shidao (ST 28), Diji (SP 8), Zusanli (ST 36), Shenshu (BL 23), Dachangshu (BL 25) and Ciliao(BL 32) in combination with herbal enema effective to clear heat and relieve toxin, activate blood and resolve stagnant blood.After 2-course treatment, 13 cases were cured and 2 cases were improved.

Objective To investigate the effects of Benazepril on inflammatory cytokines in patients with primary hypertension and its efficacy analysis.Methods 72 patients with mild to moderate primary hypertension were randomly divided into benazepril group(37 cases)and control group(35 cases).The control group patients were given felodipine sustained release tablets Stag,1 times daily.Benazepril group were given benazepril tablets 10mg,1 times daily.Both groups were treated for 8 weeks.Changes in blood pressure and heart rate,plasma inflammatory cytokine levels and the adverse reactions were observed.Results After 8 weeks of treatment,the patients' blood pressures were significantly decreased (t =2.87,2.43,2.31,2.23,P ＜ 0.05 or P ＜ 0.01) than before in both groups.And the decrease in the benazepril group was more obvious than that in the control group (t =2.21,2.13,all P ＜ 0.05),while the patients' heart rates in the two groups showed no significant change (P ＞ 0.05) ; Patients' plasma IL-4 and IL-8 levels decreased significantly,plasma IL-10 levels increased significantly (t =2.99,2.87,3.21,2.13,2.19,2.23,all P＜0.05 or P＜0.01),and the magnitude of decrease or increase in the benazepril group were more obvious compared with the control group (t =2.21,2.13,2.34,all P ＜ 0.05).Conclusion Benazepril in the treatment of primary hypertension has good antihypertensive effect and security which can significantly reduce the plasma levels of proinflammatory cytokines IL-4 and IL-8 levels,improve the anti-inflammatory cytokine IL-10 levels,and has the role of suppressing the inflammatory response of the vessel wall.

Next generation sequencing (NGS) based test provided us an unprecedented power for the molecular diagnosis of rare diseases with genetic etiology, thus enabling the practice of precision medicine.In order to maximize the clinical utilities of NGS-based test, attention should be paid to the improvement of the sequencing technology, data quality and the accuracy of data interpretation, as well as the clinical correlations and pre-and post-test genetic counseling services.In doing so, it is believed practice of NGS-based test can bring expected benefit to many patients and families.

OBJECTIVE Through detecting hepatitis B virus markers and hepatitis B virus DNA,to probe into the significance of HBV-DNA detection in HBsAg negative blood donors for blood transfusion safety.METHODS ELISA was used for detecting hepatitis B virus markers,and real-time fluorescent quantitative PCR assay was introduced for measuring HBV-DNA.RESULTS Totally 1 698 samples from HBsAg negative blood donors were examined,the positive rate of HBV-DNA was 2.71%.The rate of the HBsAb positive and HBcAb positive group,the HBsAb positive,HBeAb positive and HBcAb positive group,the HBcAb positive group,the HBeAb positive and HBcAb positive group,the HBsAb positive group and the all hepatitis B virus markers negative group were 5.71%,3.60%,6.60%,2.77%,38.48% and 46.94%,respectively.The positive rate of HBV-DNA in these groups were 7.22%,8.20%,4.46%,8.51%,1.21% and 2.26%,respectively.CONCLUSIONS It′s very important for blood transfusion safety to detect HBV-DNA in HBsAg negative blood donors.The more economic,reasonable and effective detecting method should be developed for blood transfusion safety.

Objective To detect the expression of oh8dG in gastric tissues and discuss the carcinogenicity of Hp.Methods oh8dG was detected with immunohistochemistry in Hp positive and Hp negative tissues.Results In immunohistochemistry,the positive rates of oh8dG expression in Hp positive and Hp negative tissues were 78% and 8%,respectively(P

OBJECTIVE:To probe into the economic-effectiveness of different therapeutic schemes in the treatment of lower respiratory tract infections(LRTI).METHODS:86cases of LRTI were randomly divided into 2 groups:Group A received intravenous cefuroxime sodium while Group B received intravenous cefuroxime sodium and oral cefuroxime axetil sequential therapy.Cost-effectiveness of the 2 schemes was analyzed.RESULTS:The costs of the 2 therapeutic schemes were 677.60 yuan and 439.58 yuan(P0.05)respectively;the cost-effectiveness ratio of the 2 were 7.67 and 5.11 respectively.CONCLUSION:Scheme B was better than Scheme A in the treatment of lower respiratory tract infection.

The antineoplastic drugs pingyangmycin ( PYM ) and adriamycin ( ADM ) and biological response modifier thymosin were tested for the effects on concanavalin A ( Con-A ) induced interleukin-2 ( IL-2 ) production by MTT colorimetric assay. When the drug was added to splenocytes of BALB/c mice in the presence of suboptimal dose of Con-A for 24h ,PYM increased IL-2 release over a wide range of doses, and this effect was achieved when using less than 0.01mg/L PYM. However, 10mg/L PYM inhibited IL-2 activity in the supernatant of cultures. ADM was able to enhance the production of IL-2, and thymosin enhance production of IL-2 too.When PYM or ADM applied with thymosin, the additive effects were shown. The activity of IL-2 was increased significantly.

OBJECTIVE:To discuss the working pattern and importance of providing pharmaceutical care for medical staff and patients. METHODS:The practical experiences of providing integrated pharmaceutical care for medical staff and patients in our hospital were summarized. RESULTS&CONCLUSION:Only new working pattern of human- oriented hospital pharmacy is established and pharmaceutical care for medical staff and patients is provided,does the combination of pharmacists,doctors and nurses in the medical practice realize.

This paper reports on the effect of the culture supernatant of NPC cell line (CNE-2S) (400?l/ml) on cAMP、IL-2、and IL-2 receptor in normal lymphocyts. The cAMP levels of lymphocytes from 11 cases was 13.77?4.92pm/1?10~6. With lymphocytes suspensives added to the CNE-2S, the cAMP levels was increased to 26.1? 15.14Pm/1?10~6(P0.05)These results show that the immune function abnormality of the patients with NPC relates to the fact of that the NPC immunologic inhibitory factor declines the ability of lymphocytes to produce IL-2.

OBJECTIVE: To evaluate the utilization and its trend of narcotic analgetics in this hospital.METHODS: The use of narcotic analgetics during the period 2005-2007 in our hospital was analyzed retrospectively.RESULTS: The consumption quantity of narcotic analgetics in our hospital increased year-on-year,much as in the consumption of morphine preparations,with oral morphine preparations dominating the 3 first places over the 3 years,however,the increase of pethidine was slowed down in consumption quantity.CONCLUSION: The use of narcotic analgetics in our hospital is rational on the whole,but measures should be taken to tighten the control on the use of narcotic analgetics.