Objective: To explore the impact of stage recovery training on children with autism spectrum disorder, and to provide reference for the scientific and rational rehabilitation and recovery training plans. Methods: A total of 59 children with autism spectrum disorder in Luzhou were recruited and randomly divided into control group, intervention group Ⅰ and intervention group Ⅱ for 4 stages of T1-T4 recovery training. Before and after the training, Autism Behavior Checklist(ABC) was used to assess child s sensation, language, motor, communication and self care ability. Results: There was no significant difference in the overall score of the ABC among the three groups in T1 and T2 stages ( P >0.05), but there was significant difference in T3 and T4 stages ( F= 3.98, 5.09, P < 0.05), which showed the intervention group Ⅰ and Ⅱ were lower than the control group. In terms of dimensions, in addition to the insignificant difference in language scores ( P >0.05), there were significant differences in feeling, communication, movement, and self care scores ( P <0.05). Overall score of the ABC in intervention group I and group II was lower than the control group ( P <0.05). Compared with the control group, the overall scores of children in intervention Ⅰ and intervention Ⅱ were significant in both T3 and T4 stages ( P <0.05). Compared with T1 stage, there were no significant differences in the overall scores of children in the control group in T2 stage, T3 stage and T4 stage ( P >0.05), while significant difference was observed in the intervention group Ⅰ and group Ⅱ in T2-T4 stage ( P <0.05). Conclusion Stage recovery training might help to alleviate the overall symptoms of children with autism spectrum disorder in sensation, communication, motor development and self care behaviors. The stage recovery training focusing on physical exercise can effectively alleviate the physical function of the child, while those focusing on social interaction is more conducive to improving child s social ability.

OBJECTIVE: To verify a rare allele of human leukocyte antigen (HLA) and analyze its inheritance and 3D molecular structure. METHODS: PCR-sequence-based typing, PCR-single strand oligonucleotide polymorphism and single allele-specific sequencing were carried out to characterize the rare HLA-C allele and its transmission in the family. Its protein structure was modeled by using SWISS-MODEL, Phyre2 and FATCAT software. RESULTS: Analysis indicated that the rare allele (HLA-C*08:84) has transmitted from the proband's mother and has differed from HLA-C*08:01 by a single base (g.512G>C), resulting in substitution of an amino acid (p.Trp147Ser). Modeling of the 3D structure of the encoded protein indicated that the amino acid residue variation is located at the alpha 2 helix, which participates the formation of pocket F. Modeling of the structures of C*08:84, C*08:01, C*08:02, C*08:03 and C*08:22 has suggested significant variation in the peptide binding regions of the backbone, with root mean square errors being 1.70 nm, 1.79 nm, 0.71 nm and 1.70 nm, respectively. CONCLUSION A rare HLA-C*08:84 allele has been identified, and its clinical significance has been analyzed.
Alleles ; Base Sequence ; HLA-B Antigens/genetics* ; HLA-C Antigens/genetics* ; Humans ; Molecular Structure ; Sequence Analysis, DNA