Author investigated the deposition of immunoglobulins and complements in thc skin of 56 patients with 19 various dermatoses by direct immunofluorescent (DIF) staining. The biopsied specimens were quick-frozen(by dry ice-acetone) and stained with FITC conjugated antihuman immunoglobulin and complement after cutting in a cryostat at -20C~30C. (countinued...)
Complement System Proteins ; Dronabinol ; Fluorescein-5-isothiocyanate ; Humans ; Immunoglobulins ; Skin ; Skin Diseases*

In order to isolate the keratinophilic fungi from the soil, 7x10cm vinyl envelope were used in shich 10 rhomboid shaped holes (1x1cm) were made, so that when the human scalp hairs as baiting material were laid out straight in the envelope the keratinophilic fungi would be baited to the open part of the hairs in envilope in the 6 different places for 20 days in Kyungpood National University Hospital campus from April 1967 to September 1967. The author's newly improved baiting method with the hairs in the vinyl envelope was simple and the results were more satisfactory for isolating keratinophilic fungi compared with the original Vanvreuseghem's hair baiting method.
Fungi* ; Hair ; Humans ; Scalp ; Soil

Clofazimine (Lamprene or B668) is a phenazine congener that is used in leprosy, and the patients treated with the drug may develop red discoloration, dark brown pigmentation and ichthyotic skin. The authors observed 181 leprosy patients who had developecl discoloration, pigmentation and ichthyotic skin during clofazimine therapy, as well as disappearance of the pigmentation after stop of it. The results are summarized as follows: 1) Reddish discoloration was most frequently observed 2 weeks after beginning treatment in 66 patients (50. 4%), which showed earlier in the higher dosage grow up, 2) Dark brown pigmentation was iviost frequently observed 4 weeks after beginning treatment in 64 patients (48. 9%), which showed earlier in the higher dosage group. R) For histopathological evaluation of pigmentation, various ataining rnethods were used. On unstained frozen sections, yellow brown crystals were scattered in the dermis of both discolored and pigmented skin, and in H R E stain pigments, melanin were niarkedly increased in the epidermal basal layer and a, faint yellow brown. ghost was seen in dermis of pigmented skin. With fat stains using oil-red-0 and Sudan III, reddish amorphous materials were scattered in and around the cytoplasm of the macrophages c>f the pigmented skin. 4) Ichthyotic skin was observed in 97 patients, and most frequently 2 and R months after beginning treatment in 26 patients (26. 8%), but it was never observec1 in 34 of all patients in spite of a continuous intake of the drug for more than a. year. -countinue-
Clofazimine* ; Coloring Agents ; Cytoplasm ; Dermis ; Frozen Sections ; Humans ; Leprosy* ; Macrophages ; Melanins ; Pigmentation ; Skin ; Sudan

Fibronectin(FN) is a major component of the extracellular matrix and is able to bind to cells and other components of the matrix. Although the cell producing the largest amounts of fibronectin is fibroblast, the pro duct,ion on FN also has been described in cultured keratinocyt,e and epithelial tumor cells of basal cell carcinoma(BCC). Recently, functional role nf FN in relation to biologic behavior of BCC and squamous cell carcinoma(SCC) is open to speculation. The authors investigated the localization of FN in the lesional skin of 5 cases of BCC, 4 of SCC, 5 of psoriasis and 5 normal skin using direct immunofluorescence technique with antifibronectin antibody to find out the production of FN in keratinocytes and tumor cells. 1. In the skin of BCC, FN was presented in a thick, linear depositions along the margin of tumor lobules in all cases, and as fillamentous deposits or scattered points in the nest of tumor cells in 3 cases. 2. In the skin of SCC, FN was presented in a thin, coarse depositions around the margin of tumor but not presented within the tumor nest in all cases. R. In psoriasis, FN was observed in horney layer and upper part of epidermis. It was presented more abundantly in dermo-epidermal junction, papillary and reticular dermis than in normal skin. 4. In normal skin, FN was absent in the epidermis but presented in dermo-epidermal junction and blood vessel wall of upper dermis, the amount of FN was decreased from papillary to lower dermis.
Blood Vessels ; Carcinoma, Basal Cell* ; Carcinoma, Squamous Cell ; Dermis ; Epidermis ; Extracellular Matrix ; Fibroblasts ; Fibronectins* ; Fluorescent Antibody Technique, Direct ; Keratinocytes ; Psoriasis* ; Skin*

A case of familial cold urticaria is reported in a 29-year-old male who showed generalized erythematous macules and papules, finally an urticarial eruption shortly after cold exposure, particularly in cold windy weather with damp. Coincidentally, systemic symptoms such as headache, fever and chills, arthralgia, and conjunctival injection were acompanied. Autosomal dominant inheritance was found on examination of his pedigree in which 11 of 18 rnembers showed similar clinical manifestations. Routme laboratory findings were within normal limits except mild leukocytosis and elevated erythrocyte sedimentation rate. Biopsy specimen from a cold-evoked lesion revealed mild ederna and mild perivascular infiltrations composed of neutrophils, eosinophils and lymphocytes in the derms, Treatments included several widely-used antihistaminics, but turned out to be unsatisfactory.
Adult ; Arthralgia ; Biopsy ; Blood Sedimentation ; Chills ; Cryopyrin-Associated Periodic Syndromes* ; Eosinophils ; Fever ; Headache ; Humans ; Leukocytosis ; Lymphocytes ; Male ; Neutrophils ; Pedigree ; Weather ; Wills

This study was done to observe the statistical difference on bullous skin disease patients (pemphigus, bullous pemphigoid, dermatitis herpetiformis, epidermolysis bullosa) who visited the department of dermatology, Kyungpook National University Hospital,from January 1966 to Deceniber 1985, For those 24 cases which tested positive under direct immunofluorescent (DIF) testing, agreement percentage of histopathologic diagnosis was compared. The results were summarized as follows : 1) 51 of 45,091 new outpatients (0.113%) were bullous skin disease patients. 2) By sex, age and monthly distribution, there was no significant difference, though epidermolysis bullosa was seen for the most part in those under 10 years of age. 3) By individual disease distribution, the pemphigus cases numbered 8, of which most cases were within the 30~60 year age group. Bullous pemphigoid cases numbered 14, of which most cases were over 50 years of age. Female patients were 2.5 times more common than male patients. Dermatitis herpetiformis cases numbered 20, and were relatively diminished in recent years. The peak age group was the 3rd decade. Epidermolysis bullosa numbered 9 cases. 4) The compatibility of the histopathologic diagnosis to the DIF diagnosis in 24 DIF positive cases indicated that of DIF positive pemphigus the histopathologic diagnrsis agreed with the DIF diagnosis in 75%, of bullous pemphigoid, 58% and of dermatitis herpetiformis, 50%.
Dermatitis Herpetiformis ; Dermatology ; Diagnosis ; Epidermolysis Bullosa ; Female ; Gyeongsangbuk-do ; Humans ; Male ; Outpatients ; Pemphigoid, Bullous ; Pemphigus ; Skin Diseases, Vesiculobullous* ; Statistics as Topic*

In order to study immunological responses in psoriatic lesions, direct immunofluorescence(DIF) findings in stratum corneum of psoriatic lesions are compared with histopathological changes. The results are summerized as follows: 1) Deposition of Igs or C 3 in stratum corneum of psoriatic lesions was demonstrated in 14(56%) of 25 psoriasis patients. IgG was present in 40% IgA in 48%, IgM in 24%, and C 3 in 32%. However, DIF findings are negative in the str,atum corneum in the uninvolved skin of psoriasiis. In control groups of 12 cases of various skin diseases, DIF findings were negative in the stratum corneum except 1 case of a patient with acne rosacea. 2)DIF findings in 22 cases of histopathologic changes of psoriatic lesions were found in 50% of hyperkeratosis in 48% of parakeratosis in 50% of Munro microabscess, in 50%, of spongiform pustule, and in 57% of the absence of stratum granulosum: ie, about only 50% were positive in DIF results. Histopathologic changes in 11 cases of positive DIF finding in psoriatic lesions were hyperkeratosis in 100%, parakeratosis in 91%, Munro microabscess in 64%, spongiform pustule in 55%, and the absence of stratum granulosum in 73%.
Humans ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Parakeratosis ; Psoriasis ; Rosacea ; Skin ; Skin Diseases

The authors investigated the incidence of stratum corneum and upper epidermal cytoplasmic antibodies with 30 untreated and 20 treated psoriasis sera, and normal human sera using normal human skins of 5 different sites and psoriatic lesion by the method of indirect immunofluorescence in order to evaluate immunologic responses i n psoreasis. The results are summarized as follows . I) The positivity of stratum corneum antibodies in untreated psoriasis sera(78.7%) was si, nificantly higher than that in normal sera(64.0%). The incidence of stratum corneum antibodies in untreated psoriasis sera was found to be the highest in the arm, followed by the scalp, leg, abdomen, and face as substrate. 2) The positivity of stratum corneum antibodies in psoriasis and normal human sera was significantly higher when tested with the psoriatic lesion as substrate than norma! skin as substrate, 3) The titer of stratum corneum antibodies in 5 sera using human skin obtained from 5 different sites on the body as substrate are the highest in the arm, and leg, and(in decreasing order of frequency) the scalp, abdomen, and face. 4) The positivity of upper epidermal cytoplhsmic antibodies in normal human sera (40.7%) was significa.ntly higher than that in untreated psoriasis sera(21.3%). 5) Ir.. the majority of cases, upper epidermal cytoplasmic antibodies coexisted with straturn corneum antibodies in the sera of patients with psoriasis and in the sera of normal humans.
Abdomen ; Antibodies* ; Arm ; Cytoplasm* ; Fluorescent Antibody Technique, Indirect ; Humans ; Humans* ; Incidence* ; Leg ; Psoriasis* ; Scalp ; Skin

For the purpose of studying the compiarson of normal persons with patients with malignant tumors, the author investigated the incidence of epidermal cytoplasmic antibodies (EC-Ab) with sera taken from 15 normal persons, 38 patients with malignant tumors, and 2 patients with miscellaneous disorders by method of indirect immunofluorescence. The results were as follow: 1) .Positive reactions of EC-Ab were found in 2(13%) of 15 normal persons and 9 (24%) of 38 patients with malignant tumors. 2)Incidence of positive reactions of EC-Ab in various types of malignant turnors were found in 3(75%) of 4 patients with lung cancer, 2(40%) of 5 patients with rectal cancer, 3(13%) of 24 patients with stomach cancer, and 1(100%) patient with Hodgkins lymphoma. 3) Among 9 patients with malignant tumors of positive EC-Ab, 5 patients yielded the upper epiderrnaI cytoplasrnic (UEC) antibodies, 3 patients the general epidermal cytoplasmic (GEC) antibodies, and 1 patient basal cell layer (BCL) antibody. 4) A.mong 9 patients with malignant tumors of positive, the deposition of IgG was discovered in 7 patients, IgA in 1 patient and C, in 1 patient.
Antibodies* ; Cytoplasm* ; Fluorescent Antibody Technique, Indirect ; Hodgkin Disease ; Humans ; Immunoglobulin A ; Immunoglobulin G ; Incidence ; Lung Neoplasms ; Rectal Neoplasms ; Stomach Neoplasms

Cutis marmorata telangiectatica congenita is a very rare congenital vascular anomaly, characterized by vascular mottling of the skin, telangiectasia, hemangioma and skin atropy at birth. The mottling may be generalized or localized in distribution and tends to fade spontaneously with age. A case of CMCT occuring in a 5 months old girl shows localized, reticuhted mottling of the skin and telangiectasia from the left buttock to the leg and s!in atrophy on the left knee. There was no other anomaly except the finger-tip sized capillary hemangioma on the letf scapular area and no change in the course for 8 months.
Atrophy ; Buttocks ; Female ; Hemangioma ; Hemangioma, Capillary ; Humans ; Infant ; Knee ; Leg ; Parturition ; Skin ; Telangiectasis