In order to isolate the keratinophilic fungi from the soil, 7x10cm vinyl envelope were used in shich 10 rhomboid shaped holes (1x1cm) were made, so that when the human scalp hairs as baiting material were laid out straight in the envelope the keratinophilic fungi would be baited to the open part of the hairs in envilope in the 6 different places for 20 days in Kyungpood National University Hospital campus from April 1967 to September 1967. The author's newly improved baiting method with the hairs in the vinyl envelope was simple and the results were more satisfactory for isolating keratinophilic fungi compared with the original Vanvreuseghem's hair baiting method.
Fungi* ; Hair ; Humans ; Scalp ; Soil

Author investigated the deposition of immunoglobulins and complements in thc skin of 56 patients with 19 various dermatoses by direct immunofluorescent (DIF) staining. The biopsied specimens were quick-frozen(by dry ice-acetone) and stained with FITC conjugated antihuman immunoglobulin and complement after cutting in a cryostat at -20C~30C. (countinued...)
Complement System Proteins ; Dronabinol ; Fluorescein-5-isothiocyanate ; Humans ; Immunoglobulins ; Skin ; Skin Diseases*

Clofazimine (Lamprene or B668) is a phenazine congener that is used in leprosy, and the patients treated with the drug may develop red discoloration, dark brown pigmentation and ichthyotic skin. The authors observed 181 leprosy patients who had developecl discoloration, pigmentation and ichthyotic skin during clofazimine therapy, as well as disappearance of the pigmentation after stop of it. The results are summarized as follows: 1) Reddish discoloration was most frequently observed 2 weeks after beginning treatment in 66 patients (50. 4%), which showed earlier in the higher dosage grow up, 2) Dark brown pigmentation was iviost frequently observed 4 weeks after beginning treatment in 64 patients (48. 9%), which showed earlier in the higher dosage group. R) For histopathological evaluation of pigmentation, various ataining rnethods were used. On unstained frozen sections, yellow brown crystals were scattered in the dermis of both discolored and pigmented skin, and in H R E stain pigments, melanin were niarkedly increased in the epidermal basal layer and a, faint yellow brown. ghost was seen in dermis of pigmented skin. With fat stains using oil-red-0 and Sudan III, reddish amorphous materials were scattered in and around the cytoplasm of the macrophages c>f the pigmented skin. 4) Ichthyotic skin was observed in 97 patients, and most frequently 2 and R months after beginning treatment in 26 patients (26. 8%), but it was never observec1 in 34 of all patients in spite of a continuous intake of the drug for more than a. year. -countinue-
Clofazimine* ; Coloring Agents ; Cytoplasm ; Dermis ; Frozen Sections ; Humans ; Leprosy* ; Macrophages ; Melanins ; Pigmentation ; Skin ; Sudan

Fibronectin(FN) is a major component of the extracellular matrix and is able to bind to cells and other components of the matrix. Although the cell producing the largest amounts of fibronectin is fibroblast, the pro duct,ion on FN also has been described in cultured keratinocyt,e and epithelial tumor cells of basal cell carcinoma(BCC). Recently, functional role nf FN in relation to biologic behavior of BCC and squamous cell carcinoma(SCC) is open to speculation. The authors investigated the localization of FN in the lesional skin of 5 cases of BCC, 4 of SCC, 5 of psoriasis and 5 normal skin using direct immunofluorescence technique with antifibronectin antibody to find out the production of FN in keratinocytes and tumor cells. 1. In the skin of BCC, FN was presented in a thick, linear depositions along the margin of tumor lobules in all cases, and as fillamentous deposits or scattered points in the nest of tumor cells in 3 cases. 2. In the skin of SCC, FN was presented in a thin, coarse depositions around the margin of tumor but not presented within the tumor nest in all cases. R. In psoriasis, FN was observed in horney layer and upper part of epidermis. It was presented more abundantly in dermo-epidermal junction, papillary and reticular dermis than in normal skin. 4. In normal skin, FN was absent in the epidermis but presented in dermo-epidermal junction and blood vessel wall of upper dermis, the amount of FN was decreased from papillary to lower dermis.
Blood Vessels ; Carcinoma, Basal Cell* ; Carcinoma, Squamous Cell ; Dermis ; Epidermis ; Extracellular Matrix ; Fibroblasts ; Fibronectins* ; Fluorescent Antibody Technique, Direct ; Keratinocytes ; Psoriasis* ; Skin*

Fibronectin(FN) is a physiologic glycoprotein presenton cell surface and in connective tissue, extracellular matrix and plasma. Increased plasma FN levels have been reported in the patients with psoriasis, connective tissue disease, internal malignancy, squamous cell carcinoma and basal cell carcinoma. The author investigated the plasma FN levels in 30 patients (males 13, female 17) with psoriasis and 23 healthy subjects (male 13, female 10) by gelatin sepharose 4B affinity chromatography and Laurells rocket immunoelectrophoresis method. The results were as follows : Plasrna JN levels were 450.4+/-80.3pg/ml in the patients with psoriasis and 334.0-51.3pg/ml in healthy subjects. There was no relationship between age graups and sex. Plasrna FN levels in the patients with psoriasis were higher than those of healthy subjects(p<0.001). There was no relationship between the levels of plasma FN and clinical severity of psoriasis.
Carcinoma, Basal Cell ; Carcinoma, Squamous Cell ; Chromatography, Affinity ; Connective Tissue ; Connective Tissue Diseases ; Extracellular Matrix ; Female ; Fibronectins* ; Gelatin ; Glycoproteins ; Humans ; Immunoelectrophoresis ; Plasma* ; Psoriasis* ; Sepharose

The lupus band test(LHT) has been proposed as a diagnostic test for lupus erythematosus(LE), differentiating discoid I E from systemic LE and also has prognostic values. For the better understanding of relation between LBT and prognosis in SLE. a study was carried out in 32 SLE patients with skin lesions. Immunofluorescent study was done with biopsies taken from involved skin and uninvolved skin of patients with SLE. The results were as follows . 1) Incidences of a positive LBT were 84% in both involved and uninvolved skin and IgM was most frequently deposited(66% & 72%) in both, whereas IgA was least frequently deposited(34% & 19%) in both. 2) Deposits in DEJ of IgG, IgM, IgA and C were found with approximately same frequency in patients with different clinical manifestations and different laboratory findings. 3) Incidences of positive LBT in patients with renal disease were approximately same as in patients without renal disease. And deposits of all clssses of immuno glcbulins were found with about the same frequency in these two groups. 4) A positive LBT composed of IgG alone or IgG and other Igs were associated with an increase of anemia, ANA, anti-DNA and hypocomplementemia. Ten renal biopsies showed diffuse proliferative glomerulonephritis(GN) in focal proliferative GN in 3 and membranous CiN in 2 and no correlation to immune deposits.
Anemia ; Biopsy ; Diagnostic Tests, Routine ; Humans ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Incidence ; Lupus Erythematosus, Systemic* ; Prognosis ; Skin

There are many reports about the deposition of immunoglobulins and complement by direct immunofluorescence(DIF) in the lesion of scabies. The authors investigated the immunoglobulins and C, for the purpose of studying the immunologic reactivity of the host to scabies mites by DIF. The results are summerized as follows: Total number of the cases investigated were 20 (18 males, 2 females), of whom 11 were in their third decade of age. The clinical forms of the tested lesions were papular type (7 cases), nodular type (12 cases), and Norwegian type (1 case). The durations of the patients were mostly within 2 months, but was 9 months in 1 case of nodular type, Deposition of immunoglobulin and C, was demonstrated by DIF in 10 of 12 cases (83. 3%) of nodular type, and 1 of 7 cases (14. 3%) of papular type. The Norwegian type showed negative results. There seemed to be no relation between the duration of scabies and positive rates. Of 20 cases tested, immunoglobulin and C, were deposited in the vascular wall in 11 lesions (55%), and at the dermoepidermal junction in 3 lesions (15%). While in 1 of 7 cases of papular type both IgM and C, were observed, IgM and C, were noted in 7 and 9, respectively, in 12 cases of nodular type.
Complement System Proteins ; Humans ; Immunoglobulin M ; Immunoglobulins ; Male ; Mites ; Scabies*

Darier's disease is a congenital dyskeratosis and is as an autosomal dominant reait but a case with a family history is relatively rare and only 2 such cases have been reported in Korea. We present typical Darier's disease occuring in a mother and her 2 daughters. A 45 year-old female has shown muitiple, confluent, dirty verrucous keratotic papules on the seborrheic area and whitish papules on the sotf palate since the age of 7 year-ole. Histologically there, are corps ronds, grains and suprabssal lacunae in the epidermis.
Edible Grain ; Darier Disease* ; Epidermis ; Female ; Humans ; Korea ; Middle Aged ; Mothers ; Nuclear Family ; Palate

A case of familial cold urticaria is reported in a 29-year-old male who showed generalized erythematous macules and papules, finally an urticarial eruption shortly after cold exposure, particularly in cold windy weather with damp. Coincidentally, systemic symptoms such as headache, fever and chills, arthralgia, and conjunctival injection were acompanied. Autosomal dominant inheritance was found on examination of his pedigree in which 11 of 18 rnembers showed similar clinical manifestations. Routme laboratory findings were within normal limits except mild leukocytosis and elevated erythrocyte sedimentation rate. Biopsy specimen from a cold-evoked lesion revealed mild ederna and mild perivascular infiltrations composed of neutrophils, eosinophils and lymphocytes in the derms, Treatments included several widely-used antihistaminics, but turned out to be unsatisfactory.
Adult ; Arthralgia ; Biopsy ; Blood Sedimentation ; Chills ; Cryopyrin-Associated Periodic Syndromes* ; Eosinophils ; Fever ; Headache ; Humans ; Leukocytosis ; Lymphocytes ; Male ; Neutrophils ; Pedigree ; Weather ; Wills

For the purpose of studying the compiarson of normal persons with patients with malignant tumors, the author investigated the incidence of epidermal cytoplasmic antibodies (EC-Ab) with sera taken from 15 normal persons, 38 patients with malignant tumors, and 2 patients with miscellaneous disorders by method of indirect immunofluorescence. The results were as follow: 1) .Positive reactions of EC-Ab were found in 2(13%) of 15 normal persons and 9 (24%) of 38 patients with malignant tumors. 2)Incidence of positive reactions of EC-Ab in various types of malignant turnors were found in 3(75%) of 4 patients with lung cancer, 2(40%) of 5 patients with rectal cancer, 3(13%) of 24 patients with stomach cancer, and 1(100%) patient with Hodgkins lymphoma. 3) Among 9 patients with malignant tumors of positive EC-Ab, 5 patients yielded the upper epiderrnaI cytoplasrnic (UEC) antibodies, 3 patients the general epidermal cytoplasmic (GEC) antibodies, and 1 patient basal cell layer (BCL) antibody. 4) A.mong 9 patients with malignant tumors of positive, the deposition of IgG was discovered in 7 patients, IgA in 1 patient and C, in 1 patient.
Antibodies* ; Cytoplasm* ; Fluorescent Antibody Technique, Indirect ; Hodgkin Disease ; Humans ; Immunoglobulin A ; Immunoglobulin G ; Incidence ; Lung Neoplasms ; Rectal Neoplasms ; Stomach Neoplasms