1.Clinicopathologic study of paraganglioma.
Chinese Journal of Pathology 2006;35(8):494-496
2.Nuclear expression of S100A4 is associated with lymph node metastasis in gastric carcinoma.
Xi-yao ZHONG ; Lian-hai ZHANG ; Shu-qin JIA ; Tao SHI ; Hong DU ; Ying HU ; Gui-guo ZHANG ; Ai-ping LU ; Ji-you LI ; Jia-fu JI
Chinese Journal of Gastrointestinal Surgery 2007;10(5):454-457
OBJECTIVETo investigate the intracellular localization of S100A4 in gastric carcinoma cells and the relationship between S100A4 expression status and lymph node metastasis of gastric carcinoma.
METHODSWestern blotting analysis was performed to locate the expression of S100A4 protein in sub-fraction components of frozen tissues. S100A4 protein expression was also determined by immunohistochemical method in 131 samples of gastric cancer and 20 samples of matched metastatic lymph nodes.
RESULTSThirty-two of 131 (24.4%) gastric carcinoma showed positive S100A4 nuclear expression and 50/131 (38.2%) carcinoma showed positive cytoplasmic expression. In 32 samples with positive S100A4 nuclear expression, 30 (93.8%) carcinomas had positive lymph node metastases. S100A4 nuclear expression level was higher in gastric carcinoma with lymph node metastasis (29.1%) than that without lymph node metastasis (7.1%) (P=0.016).
CONCLUSIONNuclear expression of S100A4 is associated with lymph node metastasis of gastric carcinoma.
Cell Nucleus ; metabolism ; Humans ; Lymphatic Metastasis ; Neoplasm Staging ; S100 Calcium-Binding Protein A4 ; S100 Proteins ; metabolism ; Stomach Neoplasms ; metabolism ; pathology
3.Proteomics and its applications in the research of papillary thyroid carcinoma.
Jie SHI ; Zhao-hui LU ; Quan-cai CUI
Chinese Journal of Pathology 2007;36(10):691-693
Biomarkers, Tumor
;
metabolism
;
Carcinoma, Papillary
;
metabolism
;
Cathepsin B
;
metabolism
;
Cell Cycle Proteins
;
metabolism
;
HSP27 Heat-Shock Proteins
;
metabolism
;
Humans
;
Proteomics
;
Repressor Proteins
;
metabolism
;
S100 Calcium Binding Protein A6
;
S100 Proteins
;
metabolism
;
Serpins
;
metabolism
;
Thyroid Neoplasms
;
metabolism
4.An Immunohistochemical Study of Proliferative Disorders of Histiocytes.
Chan Il PARK ; Hee Jeong AHN ; Hoguen KIM
Yonsei Medical Journal 1988;29(1):11-16
ln an attempt to clarify the dual origin histiocytes and to reclassify histiocytic proliferative disorders according to their immunohistochemical properties, normal histiocytes and histiocytes in selected proliferative disorders were stained using the peroxidase-antiperoxidase method for lysozyme, 1-antichymotrypsin and for S-100 protein. The proliferated histocytes of cosinophilic granutoma and Letterer-siwe disease were strongly immunoreactive for S-100 protein. In histiocytic medullary reticulosis (HMR) and in histiocytic lymphoma, all three markers were found within the tumor cells. ln fibrous histiocytoma and in juvenile xanthogranuloma, only a few weakly immunoreactive cells for S-100 protein were observed. lnflammatory malignant fibrous histiocytoma(MFH) (Xanthosarcoma) and xanthoma were immunoreactive for 1-antichymotrypsin and lysozyme respectively. ln MFH of the storiform -pleomorphic type and in atypical fibroxanthoma, stains using all of the histiocytic markers were negative. These results suggest that eosinophilic granuloma. Letterer-Siew disease, fibroxanthoma and juvenile xanthogranloma are proliferative disorder of T-zone histiocytes; HMR and histiocytic lymphoma are those of pluripotential stem cells capable of dual histiocytic differentiation; xanthoma and xanthosarcoma are monocytic proliferative disease; and MFH of the storiform-pleomorphic type and atypical fibroxanthoma are not true histiocytic diseases.
Histiocytes/*metabolism
;
Human
;
Immunohistochemistry
;
Reticuloendotheliosis/classification/*metabolism/pathology
;
S100 Proteins/metabolism
;
Support, Non-U.S. Gov't
5.Necrotizing lymphadenitis--a clinico-pathologic study of 36 cases with immunohistochemical analysis.
Kyung Ja CHO ; Chul Woo KIM ; Seong Hoe PARK ; Sang Kook LEE
Journal of Korean Medical Science 1991;6(1):55-61
Thirty-six cases of necrotizing lymphadenitis--including 33 cases of unknown etiology, 1 typhoid lymphadenopathy, and 2 cases of suspicious lupus lymphadenopathy--were clinico-pathologically reviewed and analyzed with immunostaining for s-100 and lysozyme. All cases histologically showed architectural effacement by paracortical lesions composed of nuclear karyorrhexis and mononuclear cell proliferation. Immunohistochemical study revealed proliferation of lysozyme-positive macrophages in the necrotizing areas and an increase in the number of s-100-positive cells in the uninvolved paracortical areas. This observation suggests that necrotizing lymphadenitis may be a common morphologic expression of a T cell-mediated hyperimmune condition induced by diverse etiologies.
Adolescent
;
Adult
;
Female
;
Humans
;
Immunohistochemistry
;
Lymphadenitis/etiology/metabolism/*pathology
;
Male
;
Muramidase/metabolism
;
Necrosis
;
S100 Proteins/metabolism
6.Granular Cell Tumors of the Abdominal Wall.
Jung Suk AN ; Sun Hee HAN ; Sung Bae HWANG ; Ju Han LEE ; Byung Wook MIN ; Jun Won UM ; Eung Seok LEE ; Heum Rye PARK ; Young Sik KIM
Yonsei Medical Journal 2007;48(4):727-730
Granular cell tumors (GCT) are found in virtually any body site, including the tongue, skin, subcutaneous tissue, breast, rectum and vulva. However, they are rarely seen in the abdominal wall. We report here on a rare case of GCT in the rectus muscle of the abdominal wall. A 44-year-old woman presented with a non-tender, hard mass in the right lower abdominal wall. Upon microscopic examination, the tumor was found to comprise of large polygonal cells with an abundant eosinophilic granular cytoplasm and round to oval nuclei. Upon immunohistochemical staining, the large cells showed S-100 and CD68 positive granular aggregates in the cytoplasm. Many lysosomes of variable size were observed in the cytoplasm.
Abdominal Neoplasms/metabolism/*pathology
;
Adult
;
Female
;
Granular Cell Tumor/metabolism/*pathology
;
Humans
;
Immunohistochemistry
;
Rectus Abdominis/metabolism/*pathology
;
S100 Proteins/metabolism
7.Granular Cell Tumors of the Abdominal Wall.
Jung Suk AN ; Sun Hee HAN ; Sung Bae HWANG ; Ju Han LEE ; Byung Wook MIN ; Jun Won UM ; Eung Seok LEE ; Heum Rye PARK ; Young Sik KIM
Yonsei Medical Journal 2007;48(4):727-730
Granular cell tumors (GCT) are found in virtually any body site, including the tongue, skin, subcutaneous tissue, breast, rectum and vulva. However, they are rarely seen in the abdominal wall. We report here on a rare case of GCT in the rectus muscle of the abdominal wall. A 44-year-old woman presented with a non-tender, hard mass in the right lower abdominal wall. Upon microscopic examination, the tumor was found to comprise of large polygonal cells with an abundant eosinophilic granular cytoplasm and round to oval nuclei. Upon immunohistochemical staining, the large cells showed S-100 and CD68 positive granular aggregates in the cytoplasm. Many lysosomes of variable size were observed in the cytoplasm.
Abdominal Neoplasms/metabolism/*pathology
;
Adult
;
Female
;
Granular Cell Tumor/metabolism/*pathology
;
Humans
;
Immunohistochemistry
;
Rectus Abdominis/metabolism/*pathology
;
S100 Proteins/metabolism
9.Clinicopathological and immunohistochemical analysis of maxillofacial granular cell tumor.
Zeliang SHEN ; Lihong YAO ; Hongjie JIANG ; Mao LI ; Yaling TANG
West China Journal of Stomatology 2023;41(4):414-420
OBJECTIVES:
To analyze the clinicopathological features of maxillofacial granular cell tumors (GCT) with the aid of immunohistochemical staining.
METHODS:
Seven cases of maxillofacial GCT were retrospectively collated, and the microscopic morphology of maxillofacial GCT was analyzed. The expression of S-100, neuron-specific enolase (NSE), SOX-10, CD68, actin, desmin, and Ki-67 in GCT was detected by immunohistochemical staining. The cases were observed in the follow-ups after clinical treatment.
RESULTS:
All seven GCT tumors lacked envelopes and were poorly defined. Microscopically, the sizes of the tumor cells were large and appeared with inconspicuous cell membranes, forming a syncytium-like appearance. The cytoplasm was filled with characteristic eosinophilic granules. The immunohistochemical results showed that six cases were NSE-positive, five cases were S-100-positive, seven cases were CD68-positive, five cases were SOX-10-positive, one case was actin-positive, and seven cases were desmin-negative. The Ki-67 index did not exceed 5% in all cases. In the follow-up sessions, none of the six cases presented a recurrence.
CONCLUSIONS
Maxillofacial GCT has a characteristic histological structure. Immunohistochemical S-100, CD68, and other indicators can assist in diagnosis, and the prognosis is good after clinical resection.
Humans
;
Ki-67 Antigen/metabolism*
;
Granular Cell Tumor/surgery*
;
Retrospective Studies
;
Actins/metabolism*
;
Desmin/metabolism*
;
S100 Proteins/metabolism*
10.Diffuse Ganglioneuromatosis of the Colon Presenting as a Large Subepithelial Tumor in Adults: Report of Two Cases.
Tae Jun KIM ; Hyun LIM ; Ho Suk KANG ; Sung Hoon MOON ; Jong Hyeok KIM ; Choong Kee PARK ; Mi Jung KWON ; Bong Hwa LEE
The Korean Journal of Gastroenterology 2015;66(2):111-115
Colonic diffuse ganglioneuromatosis is a benign neoplastic condition characterized by disseminated, intramural, or transmural proliferation of neural elements involving the enteric plexuses, sometimes associated with von Recklinghausen's disease and other multiple tumor syndromes. Colonic diffuse ganglioneuromatosis is usually large, ranging from 1 to 17 cm, and thus can distort the surrounding tissue architecture as well as infiltrate the adjacent bowel wall. However, colonic diffuse ganglioneuromatosis is an exceptional finding in adults and only individual cases are reported in the literature. Herein, we report two unusual cases of adult patients with colonic diffuse transmural ganglioneuromatosis presenting as a large subepithelial tumor.
Adult
;
Aged
;
Colon/metabolism/*pathology
;
Colonoscopy
;
Ganglioneuroma/*diagnosis/metabolism/pathology
;
Humans
;
Immunohistochemistry
;
Male
;
S100 Proteins/metabolism
;
Tomography, X-Ray Computed