We analyzed the endosteal morphology of the proximal femur in 300 Korean adults radiologically and assessed the proximal canal filling of the cementless anatomic stem in 100 patients with imaging analysis system. The cases were divided in six groups, according to age by the decades. The canal flare indices were 4.20, 3.82, 3.47, 3.40, 3.34 for each age group from third to eighty decades respectively. The shape of proximal cannal in the 300 cases, showed 234 cases(78%) to be normal, while 30 cases(10%) in the Stovepipe configuration and 36 cases(12%) in the Champagne flute appearance. The proximal canal filling rate in an AP view radiologically measured from the level of the lesser trochanter to 2cm distal to the lesser trochanter in 100 patients with cementless anatomic stem were s follow : 60 to 69% range in 9 cases(9%), 70 to 79% in 58 cases(58%), 80 to 89% in 31 cases(31%) and 90 to 100% in 2 cases(2%). With regard to the suitability of cementless femoral components, anatomic fitting, distal fitting, proximal fitting, undersized stem were 66%, 23%, 5%, 6%, respectively. It is difficult to achieve the optimal anatomic fitting to the femoral cannal with one design of the cementless femoral stem, because of the greater variation of proximal femoral anatomy in normal population.
Adult ; Femur ; Humans

To examine the fate of cryopreserved meniscus used as orthotopic allograft on alternative to total removal of menisci, the authors have performed replacement of medial meniscus with cryopreserved one which had been preserved at −70℃ for 7 days in right side and with fresh autograft without cryopreservation in left side as a control group.Transplanted menisci were sampled at 4, 8, 12 ,16 weeks, postoperatively to observe their gross and histological findings. At 4 weeks, the allograft was healed to the perioheral capsular tissue and meniscal horn attachments of the host by fibrovascular scar tissue retained their normal appearance in gross findings. It showed an increased cellularity immediately adjacent to its peripheral attachment covered by the vascularized synovial tissues. At 8 weeks, it showed even distribution of cellularity in histological finding. At 12 weeks, gross findings of mild shrinkage and degeneration were seen and those were covered by both synovial and connective tissue with regenerated chondrocyte in histological findings. At 16 weeks, a little difference between allograft and normal meniscus was showed in gross and histological findings. There are regenerated chondrocytes in the deep area some degenerative changes were noted in the tibial and femoral cartilage not covered by meniscus. Above results showed cryopreserved meniscal allograft was able to regenerate and clinically suggest good treatment modality in case of unable to reshape to normal structure after total meniscectomy.
Allografts ; Animals ; Autografts ; Cartilage ; Chondrocytes ; Cicatrix ; Connective Tissue ; Cryopreservation ; Horns ; Knee ; Menisci, Tibial

Rhipicephalus sanguineus, commonly known as brown dog tick is a widespread species with considerable public health and economic importance. Tremendous efforts were performed to control the tick populations with the concern of resistance build-up and environmental issues. Alternative towards microbial control thus emerged as one option to reduce tick populations. In this study, the ovicidal efficacy of a native isolate entomopathogenic hyphomycetes fungi, Metarhizium anisopliae strain HSAH5 was evaluated against eggs of R. sanguineus. Spray applications with three different conidial concentrations of 105, 106 and 107 conidia mL-1; 40 ppm of Flumethrin and a negative control. The M. anisopliae strain was found highly virulent to R. sanguineus eggs by reducing the hatching percentages to ≈30% compared with 8.9% in Flumethrin eggs. The result showed a significantly higher mortality in M. anisopliae group than those of the control groups (F = 42.08, df = 32, P < 0.001) at 30 days post-infection. However, there are no significant differences within the M. anisopliae group, in which the mortality between different conidial concentrations is almost the same. The estimated LC50 of M. anisopliae against eggs of R. sanguineus is 1.36 × 103 conidia ml-1. Thus, these results suggest M. anisopliae strain HSAH5 could be a potential biocontrol agent of R. sanguineus in the integrated approach to managing ticks in the residential landscape by targeting on the eggs.

Toxoplasma gondii is an important zoonotic parasite causing significant health problems to humans and animals. In recent years, a number of investigations about the seroprevalence of T. gondii in China have been reported, but little is known on the prevalence of toxoplasmosis in sheep in northern China. In the present study, a total of 288 sheep serum samples were collected from Inner Mongolia, Heilongjiang, Jilin and Hebei provinces of northern China for T. gondii antibody survey using a latex agglutination test (LAT). Of these, 87 (30.2%) serum samples were positive for antibodies to T. gondii, and the antibody titres ranged from 1:64 to 1:1,024. Seroprevalence of T. gondii infection in sheep was 17.1% in Inner Mongolia, 33.8% in Heilongjiang, 24.6% in Jilin and 46.3% in Hebei. Age and rearing system significantly affected seropositivity. The present survey indicates antibodies to T. gondii are widely prevalent in sheep in northern China, which may cause public health problems in these provinces.