1.PCR analysis of Yq microdeletions in infertile males, a study from South India.
S Ramesh BABU ; M SWARNA ; P PADMAVATHI ; P P REDDY
Asian Journal of Andrology 2002;4(4):265-268
AIMTo estimate the frequency of microdeletions in the long arm of Y-chromosome of 20 infertile males from South India.
METHODSPolymerase chain reaction (PCR) amplification using Y-specific STS of azoospermia factor (AZF) regions i.e., SY 84 for AZFa, SY 127 for AZFb and SY 254 for AZFc.
RESULTSOf the 20 infertile subjects 3 (15 %), one azoospermic and two oligozoospermic, showed microdeletions in the AZF region of Y-chromosome.
CONCLUSIONThe frequency of deletions involving AZF region of the Y-chromosome is 15 % in azoospermic and severely oligozoospermic infertile men. PCR amplification of AZF locus is useful for the diagnosis of microdeletions in the Y-chromosome.
Base Sequence ; Chromosome Deletion ; Chromosome Mapping ; Chromosomes, Human, Y ; DNA Primers ; Female ; Gene Frequency ; Genetic Loci ; Humans ; India ; Infertility, Male ; genetics ; Male ; Oligospermia ; genetics ; Polymerase Chain Reaction ; methods ; Reference Values ; Seminal Plasma Proteins ; genetics
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