1.Inhibitory Effect of Two Alkaloids, (-)-Corydalmine and (-)-Isocorypalmine Isolated from Corydalis chaerophylla on Several Phytopathogenic Fungi.
Sangita SAHNI ; S MAURYA ; R N JHA ; V B PANDEY ; U P SINGH
Mycobiology 2004;32(4):160-163
Medicinal plants play important roles in controlling plant diseases as one of the safest and ecofriendly methods. These plants have been used in the form of crude extracts as well as active principles in vitro and under field conditions to control plant diseases. Among the active principles, alkaloids have shown significant antifungal activity. We have investigated the effect of two alkaloids viz., (-)-corydahnine and (-)-isocorypahnine isolated from Corydalis chaerophylla, against spore germination of some plant pathogenic and saprophytic fungal spores. Significant inhibition of spore germination at 100 microg/ml was seen against Curvularia penniseti, Curvularia sp. and Colletotrichum gloeosporioides by (-)-corydahnine but (-)-isocorypalmine was also effective against fungi included in the experiment.
Alkaloids*
;
Colletotrichum
;
Complex Mixtures
;
Corydalis*
;
Fungi*
;
Germination
;
Plant Diseases
;
Plants
;
Plants, Medicinal
;
Spores
;
Spores, Fungal
2.Effect of the Mixture of Two Plant Alkaloids Isolated from Corydalis longipes Against Balsam Powdery Mildew on Detached Leaves and Pea Powdery Mildew in Field.
Leena GOHAIN ; S MAURYA ; M B PANDEY ; V B PANDEY ; U P SINGH
Mycobiology 2004;32(4):155-159
N-Methylhydrasteine hydroxylactam and 1-methoxyberberine chloride, both alkaloids, extracted from Corydalis longipes have been assayed for their activities against two powdery mildews. The spore germination of Erysiphe cichoracearum on detached leaf of balsam(Impatiens balsaminia) following pre- and post-inoculation treatments by their mixture has shown high efficacy against the pathogen at 100, 200 and 300 microg/ml. The mixture was also effective at both pre- and post-inoculation treatments at 500, 1000, 1500 microg/ml doses against E. pisi causing pea powdery mildew in pea(Pisum sativum) under field conditions. The significant efficacy of the mixture of two compounds against spore germination on detached leaves of balsam and also under field conditions in pea warrants its inclusion in trials against some other diseases under field conditions.
Alkaloids*
;
Corydalis*
;
Germination
;
Peas*
;
Plants*
;
Spores
3.Antifungal Activity of Narceine Methyl Ester and Narceine Isolated from Corydalis longipes Against Some Phytopathogenic Fungi.
Dibyendu CHOWDHURY ; S MAURYA ; M B PANDEY ; V B PANDEY ; B K SARMA ; U P SINGH
Mycobiology 2005;33(4):206-209
Narceine methyl ester and narceine are potent alkaloids which were isolated from Corydalis longipes were found effective in vitro at very low concentration, i.e., 100~500 ppm against spore germination of some test plant pathogenic fungi (Alternaria solani, A. tagetica, Cercospora abelmoschi, Curvularia maculans, Erysiphe cichoracearum, E. pisi, Fusarium udum, Helminthosporium oryzae, H. penniseti, Ustilago cynodontis). Among the test, phytopathogens the spores of F. udum, C. maculans and H. penniseti were highly sensitive at 200 ppm. However, spores of E. pisi, A. solani and A. tagetica were less sensitive at low concentration followed by other test fungi. Most of the fungi showed zero or nearly zero percent spore germination at 400 and 500 ppm.
Alkaloids
;
Corydalis*
;
Fungi*
;
Fusarium
;
Germination
;
Helminthosporium
;
Oryza
;
Plants
;
Spores
;
Ustilago
4.Effect of Ent-norsecurinine, an Alkaloid, on Spore Germination of Some Fungi.
Mitul GOEL ; S MAURYA ; V B PANDEY ; V P SINGH ; A K SINGH ; U P SINGH
Mycobiology 2002;30(4):225-227
The inhibitory activity of ent-norsecurinine alkaloid was evaluated against spore germination of some plant pathogenic fungi (Curvularia maculans, Curvularia species, C. palliscens, Colletotrichum gloeosporioides, Colletotrichum species, Alternaria solani, A. brassicae, Fusarium udum, Helminthosporium echinoclova and H. penniseti). It inhibited spore germination of all the test fungi. C. maculans, C. species, and C. palliscens were the most sensitive as complete inhibition of spore germination was observed at 1000 ppm. A. solani was not inhibited by this chemical.
Alternaria
;
Brassica
;
Colletotrichum
;
Fungi*
;
Fusarium
;
Germination*
;
Helminthosporium
;
Plants
;
Spores*
5.Antifungal Activity of Nor-securinine Against Some Phytopathogenic Fungi.
Sangita SAHNI ; S MAURYA ; U P SINGH ; A K SINGH ; V P SINGH ; V B PANDEY
Mycobiology 2005;33(2):97-103
Crude extracts and active principles from medicinal plants have shown potential role in controlling plant diseases in glasshouses as well as in fields as one of the safest and ecofriendly methods. The effect of nor-securinine (an alkaloid) isolated from Phyllanthus amarus has been seen against spore germination of some fungi (Alternaria brassicae, A. solani, Curvularia pennisetti, Curvularia sp., Erysiphe pisi, Helminthosporium frumentacei) as well as pea powdery mildew (Erysiphe pisi) under glasshouse conditions. The sensitivity of fungi to nor-securinine varied considerably. Nor-securinine was effective against most of the fungi. H. frumentacei was more sensitive even at the lowest concentration (1,000 microg/ml). Likewise conidia of E. pisi were also inhibited in partially or completely appressorium formation. Pre-inoculation treatment showed greater efficacy than post-inoculation in inhibiting powdery mildew development on pea plants in a glasshouse. Maximum inhibition occurred at 2000 microg/ml
Brassica
;
Complex Mixtures
;
Fungi*
;
Germination
;
Helminthosporium
;
Peas
;
Phyllanthus
;
Plant Diseases
;
Plants, Medicinal
;
Spores
;
Spores, Fungal