Objective To determine the socio-demographic and clinical correlates of suicide attempts in Chinese schizophrenia outpatients and their impact on patients' quality of life (QOL).Methods Two hundred and fifty eligible subjects were recruited and interviewed using standard instruments. Multiple logistic regression with stepwise method was used.Results The prevalence of suicide attempts was 33.6%. Patients with a history of suicide attempts were more likely to have multiple hospitalizations and poor physical QOL domain. Negative symptoms inversely predicted suicide attempts.Conclusion Suicide attempts could be controlled if more attention is paid on schizophrenia patients with multiple hospitalizations and poor physical QOL domain, which could facilitate the re-entry to community for schizophrenia outpatients in China.

Objective To investigate the effect of propofol on cognitive function in young obese rats, and to explore its relationship with heme oxygenase 1 (HO-1), superoxide dismutase 1 (SOD1) protein expression and plasma S100 calciumbinding protein β (S100β) expression. Methods A total of 140 male SD rats aged 21 days were randomly divided into normal diet group (n=40) and high-fat diet group (n=100), and the rats were fed with a normal diet and a high-fat diet, respectively. After 4 weeks of feeding, 40 rats of the high-fat diet group with body mass≥the average body mass ≥1.4 times of the standard deviation of the normal diet group were designated as obese rats. The rats in the normal diet group were randomly divided into the normal lipid emulsion solvent group (NL group) and the normal propofol group (NP group), and the 40 obese rats were randomly divided into the obese lipid emulsion solvent group (OL group) and the obese propofol group (OP group), with 20 rats in each group. The rats in the propofol groups were intraperitoneally given propofol 100 mg/kg, and those in the lipid emulsion solvent groups (control groups) were intraperitoneally given lipid emulsion solvent 100 mg/kg, once a day for 7 days. On the first day after drug withdrawal, Morris water maze test was performed to evaluate the spatial learning and memory abilities of rats in each group. Meanwhile, the plasma S100β protein content of each group was detected by enzyme-linked immunosorbent assay, the expression levels of HO-1 and SOD1 protein in hippocampus were detected by Western blotting, and the changes of neurons in hippocampus CA1 area were observed by hematoxylin-eosin staining. Results Compared with the OL group, the escape latency time was significantly prolonged on 1-5 days (all P<0.05), the third quadrant residence time was significantly shortened (P<0.05), the times of crossing platform was significantly decreased (P<0.05), the expression of plasma S100β protein was significantly increased (P<0.05), the relative expression levels of HO-1 and SOD1 protein in hippocampus were significantly decreased (both P<0.05), and the number of neurons in hippocampal CA1 area was significantly decreased (P<0.01) in the OP group. Compared with the NL group, the escape latency time of the NP group was significantly prolonged on 1-2 days (both P<0.05), and there were no significant differences in other indexes mentioned above (all P>0.05). Conclusion Propofol can down-regulate the expression of anti-oxidant factors HO-1 and SOD1 in the hippocampus of young obese rats, leading to increase of S100β expression and oxidative stress and eventually causing cognitive impairment..

Objective To investigate the subjective quality of life(SQOL)in schizophrenia outpatients and explore the relationships among symptomology,drug-induced extrapyramidal side effects(EPS)and SQOL.Methods198 eligible subjects were recruited and interviewed using standard instruments.Multiple linear regression with stepwise method was used.ResultsEPS was found to significantly predict physical SQOL domain while anxiety and positive symptoms predicted psychological,social and environmental SQOL domains,respectively.ConclusionSQOL of schizophrenia outpatients could be improved if their anxiety and positive symptoms and EPS are effectively controlled.

Objective To investigate the effect of mircoRNA-128-3p (miR-128-3p) on epithelial-mesencfrymal transition (EMT) of ovarian cancer cells and its regulatory mechanism on zinc finger E-box binding homobox 1(ZEB1). Methods Real-time PCR technology was used to detect the expression of miR-128-3p in epithelial ovarian cancer tissue (EOC) and adjacent normal tissue (30 cases each), and to observe whether there was a difference. Two human ovarian cancer cell lines, SK0V3 and A2780, were selected and transfected respectively. MiR-128-3p mimic (miR-128-3p mimic group) and negative control mimic (NC mimic group) were used to detect the expression of miR-128-3p in 4 groups by Real-time PCR to verify the interference effect. Transwell assay was used. The migration and invasion abilities of the four groups of cells were observed. The regulatory relationship between miR-128-3p and ZEBl was verified by dual luciferase assay, and the expression level of ZEBl protein after overexpression of miR-128-3p was detected by Western blotting; pcDNA-ZEBl was transfected into SK0V3 and A2780 cell lines to make it overexpression of ZEBl was divided into NC mimic group, miR-128-3p mimic group, and miR-128-3p mimic+pcDNA-ZEBl group. Western blotting was used to detect the EMT-related protein E-cadherin in 6 groups of cells and the expression level of vimentin. Results Real-time PCR result showed that the expression of miR-128-3p in EOC tissues decreased compared with that in adjacent tissues (P < 0. 01); The relative expression of miR-128-3p in the miR-128-3p mimic group was higher than that in the NC mimic group, while the numbers of migrating cells and invasive cells were lower than those in the NC mimic group (P < 0 . 0 1) . The result of dual luciferase experiments showed that miR-128-3p had a negative regulatory effect on ZEBl. In SK0V3 and A2780 cells, the relative expression of ZEBl protein in the miR-128-3p mimic group was lower than that in the NC mimic group, while the relative protein expression of E-cadhein was higher than that in the NC mimic group (P < 0 . 0 1) . The relative protein expression of E-cadhein in the miR-128-3p mimic+pcDNA-ZEBl group was lower than that in the miR-128-3p mimic group (P < 0 . 0 1) . In SKOV3 and A2780 cells, the relative protein expression of vimentin in the miR-128-3p mimic group was lower than that in the NC mimic group, and the relative protein expression of vimentin in the miR-128-3p mimic+pcDNA-ZEBl group was higher than that in the miR-128-3p mimic group (P < 0 . 0 1) . Conclusion The expression of miR-128-3p decreases in epithelial ovarian cancer tissues, which ma)' be due to the regulation of ZEBl to affect the expression of EMT-related proteins and participate in the EMT process of ovarian cancer cells.

Adult ; Buprenorphine ; Female ; Humans ; Male ; Middle Aged ; Narcotic Antagonists ; Narcotics ; Opioid-Related Disorders ; mortality ; Singapore ; epidemiology

Aged ; Anti-Bacterial Agents ; adverse effects ; therapeutic use ; Asian Continental Ancestry Group ; Cephalosporins ; adverse effects ; therapeutic use ; Electroencephalography ; Female ; Humans ; Kidney Failure, Chronic ; Liver Cirrhosis ; Male ; Middle Aged ; Neurotoxicity Syndromes ; diagnosis ; etiology ; Singapore

AIM: To observe the change in full-field electroretinogram(ERG)in patients with nonproliferative diabetic retinopathy(NPDR)after panretinal photocoagulation(PRP)2d and 2mo, and evaluate the effects of Difrarel protecting dark adaptation function.

METHODS: Fifty-five cases with NPDR were randomly divided into the observation group and the control group.Both groups were treated with panretinal photocoagulation.Difrarel tablets was added in the observation group,and Vitamin B was added in the control group. We administered these drugs to the patients for 2mo continuously and the changes in ERG were observed after 2d and 2mo treatment. The amplitude of wave b(bA), and the peak time of wave b(bT)were observed and the data were treated statistically with SPSS 20.0 and test t in pairs.

RESULTS: The bT of the two groups before and at 2mo treatment had no difference inner group or inter-group(P>0.05). But there was obvious difference in bA of control group at 2mo after treatment compared with before treatment(P<0.05), significantly more increased amplitude of observation group than that of control group(P<0.05).

CONCLUSION: The function of each layer of PDR's retina has been damaged obviously and cannot get well after PRP. The patients with diabetic retinopathy combined with Difrarel tablets and PRP can better restore vision and improve the dark adaptation function.

AIM: To observe the clinical effects of different methods of internal limiting membrane peeling in vitrectomy in treatment of idiopathic macular hole(IMH)with macular hole closure index(MHCI)<0.7.

METHODS: Totally 88 patients(88 eyes)with MHCI<0.7 IMH admitted from May 2014 to May 2017 were randomly divided into group A(44 eyes underwent extended internal limiting membrane dissection)and group B(44 eyes underwent standard internal limiting membrane dissection). The closure of macular hole, best corrected visual acuity(BCVA), central scotoma and complications were observed between the two groups.

RESULTS: At 6mo after operation, the closure rate of macular hole in group A was significantly higher than that in group B(91% vs 75%, P<0.05). At 6mo after operation, BCVA in group A was better than that in group B(0.47±0.05 vs 0.74±0.14, P<0.05). The percentage of central scotoma eyes was lower than that in group B(4% vs 23%, P<0.05), but there was no significant difference in the incidence of complications between the two groups(11% vs 9%, P>0.05).

CONCLUSION: Extended internal limiting membrane dissection is more effective than standard internal limiting membrane dissection in the treatment of IMH with MHCI < 0.7, and the former is better for the recovery of retinal function.

Autoimmune liver diseases mainly include autoimmune hepatitis, primary biliary cholangitis, primary sclerosing cholangitis, and various types of overlap syndromes and are often associated with extrahepatic autoimmune diseases. However, few studies have described the association of autoimmune liver diseases with rheumatic disease and/or skin disease. For this purpose, this article reviews the rheumatic and skin manifestations associated with autoimmune liver diseases in detail and the clinical significance of non-specific antibodies. It is pointed out that individualized treatment should be given to patients with rheumatic disease and/or skin disease.

AIM: To investigate the effect of docosahexaenoic acid(DHA)on photoreceptor apoptosis and phosphatidylinositol-3-kinase/threonine protein kinase(PI3K/Akt)pathway in age-related macular degeneration(ARMD)rats.

METHODS: The rats were randomly divided into blank control group, model group, low dose DHA group(L-DHA group), medium dose DHA group(M-DHA group)and high dose DHA group(H-DHA group). The rat model of dry ARMD was established by light injury. He staining was used to observe the pathological changes of retina, TUNEL method to detect the apoptosis of retinal cells, transmission electron microscopy to observe the ultrastructure of retinal ganglion cells, enzyme-linked immunosorbent assay to detect the levels of TNF - α and IL-6 in retina, Western blot to detect the expression of p-PI3K, p-Akt, Bax, Bcl-2, p-NF-κBp65 and cleved-caspase-3 protein in retina.

RESULTS: Compared with the blank control group, the total thickness of retina, the thickness of outer nuclear layer and inner nuclear layer, the expression of p-PI3K, p-Akt and Bcl-2 protein in the retina of the model group decreased(P<0.05), the apoptosis index in the ganglion cell layer and outer nuclear layer, the level of TNF-α and IL-6 in the retina, the expression of Bax, p-NF-κBp65 and cleared caspase-3 protein increased(P<0.05). Compared with the model group, the total thickness of retina, the thickness of outer nuclear layer and inner nuclear layer, the expression of p-PI3K, p-Akt and Bcl-2 protein in retina increased in M-DHA group and H-DHA group(P<0.05), the apoptosis index in ganglion cell layer and outer nuclear layer, TNF-α and IL-6 levels in retina, Bax, p-NF-κBp65 and cleved caspase-3 protein expression decreased(P<0.05).

CONCLUSION: DHA may inhibit photoreceptor apoptosis by activating PI3K /Akt pathway.