Aged, 80 and over ; Exanthema ; etiology ; Female ; Humans ; Leukemia, Monocytic, Acute ; pathology ; Leukemic Infiltration ; complications ; diagnosis ; Skin ; pathology

Objective: To study the effect of orthodontic treatment on alveolar bone density and epidermal growth factor (EGF) expression in patients with periodontal disease. Methods : 50 patients with periodontal disease admitted into our hospital from January 2015 to January 2016 were selected as research object. Results : Before treatment, patients were given dental health instruction. Under the conditions of patients agreeing and cooperating, patients received orthodontic treatment. The changes of alveolar bone density, EGF in blood, saliva, gingival crevicular fluid (GCF), indexes of periodontal inflammation (gingival index, papilla Bleeding index, probing depth) of patients before and after treatment for one month were compared. Before treatment, the mandibular alveolar bone density was significantly higher than maxillary alveolar bone density (P < 0.05); After treatment, the maxillary and mandibular alveolar bone density were significantly lower than before treatment, and maxillary bone density was significantly lower than mandibular bone density (P < 0.05); After one-month treatment, the concentration of EGF expression in blood, saliva compared with before treatment, there was no statistical significance (P > 0.05); After one-month treatment, the concentration of EGF expression in GCF was significantly higher than before treatment (P < 0.05); Before and after treatment for one month, the concentration of EGF expression in GCF was significantly higher than blood, saliva (P < 0.05); After treatment, the GI, PBI, PD levels were significantly lower than before treatment (P < 0.05); After treatment, flow of saliva and GCF were significantly higher than before treatment (P < 0.05). Conclusion Orthodontic treatment has certain influence on EGF expression in GCF, but has no obvious influence on EGF expression in blood and saliva; The increase of EGF expression derives from periodontal tissue cells and has close association with periodontal remodeling, periodontal tissue cells can promote self-remodeling through autogenous regulation and local humoral regulation.

Humans ; Diverticulum, Colon ; Fecal Impaction ; Hydrodynamics

AIM: To observe the clinical effects of different methods of internal limiting membrane peeling in vitrectomy in treatment of idiopathic macular hole(IMH)with macular hole closure index(MHCI)<0.7.

METHODS: Totally 88 patients(88 eyes)with MHCI<0.7 IMH admitted from May 2014 to May 2017 were randomly divided into group A(44 eyes underwent extended internal limiting membrane dissection)and group B(44 eyes underwent standard internal limiting membrane dissection). The closure of macular hole, best corrected visual acuity(BCVA), central scotoma and complications were observed between the two groups.

RESULTS: At 6mo after operation, the closure rate of macular hole in group A was significantly higher than that in group B(91% vs 75%, P<0.05). At 6mo after operation, BCVA in group A was better than that in group B(0.47±0.05 vs 0.74±0.14, P<0.05). The percentage of central scotoma eyes was lower than that in group B(4% vs 23%, P<0.05), but there was no significant difference in the incidence of complications between the two groups(11% vs 9%, P>0.05).

CONCLUSION: Extended internal limiting membrane dissection is more effective than standard internal limiting membrane dissection in the treatment of IMH with MHCI < 0.7, and the former is better for the recovery of retinal function.

AIM: To investigate the efficacy and safety of conbercept intravitreal injection combined with selective laser photocoagulation for central retinal vein occlusion(CRVO).

METHODS: A retrospective study was performed on 66 patients with CRVO in our department of ophthalmology. Among them, 30 patients received only conbercept's intravitreal injection and served as a control group. On this basis, another 36 patients were combined with selective laser photocoagulation and as an observation group. The differences of central macular thickness(CMT), BCVA(LogMAR)and complication rate after treatment were compared between the two groups.

RESULTS:The CMT and BCVA of the two groups were significantly improved after treatment(P<0.05). The CMT and BCVA of the observation group were significantly better than the control group at 6th month after treatment(P<0.05). The number of intravitreal injections in the observation group was significantly lower than that in the control group(P<0.05). There was no significant difference in the complications(P>0.05).

CONCLUSION: The combination therapy has a more significant effect on CRVO and is safe.

AIM: To investigate the clinical effect of scleral buckling surgery combined with 23G vitrectomy in treatment of intraocular foreign body with retinal detachment.

METHODS: Totally 72 cases of patients with intraocular foreign bodies and retinal detachment admitted from January 2014 to January 2018 were selected and divided into the control group(36 cases)and the observation group(36 cases)by random number table method. The patients in the control group were treated with 23G vitrectomy surgery while patients in the observation group were treated with scleral buckling surgery on the basis of the treatment in the control group. The changes of intraocular pressure(IOP)and visual acuity, successful reset, recurrence and complications were observed before operation and 3mo after silicone oil was removed.

RESULTS: There was no difference in IOP and BCVA between the two groups before operation(P>0.05). Three months after silicone oil was removed, IOP and BCVA in two groups were significantly improved compared with those before operation(P<0.05). There was no difference between the two groups(P>0.05). The success rates of one-time anatomical reduction in the observation group and the control group were respectively 97% and 81%(P<0.05). The recurrence rates of the observation group and the control group were respectively 6% and 25%(P<0.05). The incidence of complications was 22% in the observation group and 31% in the control group(P>0.05).

CONCLUSION: The treatment of scleral buckling combined with 23G vitrectomy in the treatment of intraocular foreign body with retinal detachment can significantly improve the IOP and visual acuity of patients, improve the success rate of reduction, reduce recurrence, and have high safety.

AIM:To study the curative efficacy of aspheric intraocular lens implantation after manual nuclear fragmentation in treatment of cataract and its effects on visual acuity.

METHODS: Totally 100 patients of cataract who received therapy from May 2013 to May 2016 in our hospital were selected. According to random number table, those patients were divided into the observation group(50 patients 68 eyes)and the control group(50 patients 67 eyes). The observation group was treated with small incision manual discectomy and aspheric intraocular lens implantation, the control group was treated with phacoemulsification cataract extraction and aspheric intraocular lens implantation, the operation effect of two groups were compared.

RESULTS: After operation, the corneal endothelial cell counts of the two groups decreased(P<0.05); in the observation group, the results were higher than those of the control group at 1 and 3mo after operation(P<0.05). In the observation group, the uncorrected visual acuity at 1mo after operation was better than that of the control group(P<0.05); there was no significant difference between the two groups in postoperative visual acuity after operation 3mo(P>0.05). At 1mo after surgery, the corneal astigmatism in the observation group was higher than that of the control group(P<0.05); there was no significant difference of corneal astigmatism between the two groups at 3mo after operation(P>0.05). The incidence of intraoperative complications and postoperative complications in the observation group were lower than that of the control group(P<0.05).

CONCLUSION: Aspheric intraocular lens implantation after manual nuclear fragmentation is well for cataract, which can reduce the damage to corneal endothelial cells, the complications rate is low and the postoperative visual acuity recovered well.

AIM: To observe the interaction of Ranibizumab and laser in the treatment of proliferative diabetic retinopathy(PDR).

METHODS: From December 2014 to August 2015, synchronous PDR patients with macular edema were divided into two groups. Thirty patients(48 eyes)were treated with Ranibizumab and laser, and 28 patients(45 eyes)were treated with laser alone. In the combined treatment group, FFA was reexamined 1wk after intravitreal injection of Ranibizumab. The patient then received panretinal photocoagulation(PRP). PRP was performed in the laser group alone. The best corrected visual acuity(BCVA)at 1, 4 and 8wk after treatment was observed and recorded. Macular thickness was measured by OCT.

RESULTS: The BCVA and macular thickness were significantly different between the two groups at 1, 4 and 8wk after treatment(P<0.05). In the laser alone group, the visual acuity decreased and the thickness of the macular thickness increased after 1wk. After treated 4 and 8wk, visual acuity improved, macular thickness decreased. After 1wk of intravitreal injection, FFA showed retinal leakage improvement in all eyes in the combined treatment group, with neovascularization retracted in varying degrees.

CONCLUSION:Ranibizumab combined with photocoagulation, can improve early vision, and reduce macular edema. Anti-VEGF therapy and photocoagulation may play a synergistic role to some extent. Drugs antagonized VEGF, improved macular edema and microvascular function. It can enhance the laser effect.Effective photocoagulation can further improve retinal ischemia and hypoxia, inhibit the excessive expression of VEGF.

Amyotrophic lateral sclerosis (ALS) is a progressively aggravating fatal neurodegenerative disease, and there is no radical cure. This paper reviews the current progress of ALS therapies, mainly including drug therapy, symptomatic management, stem cell transplantation and gene therapy.

Objective: To explore the mechanism of shRNA Twist gene on proliferation and invasion of lung adenocarcinoma NCI-H1299 cells. Methods: Twist siRNA interference expression vector was constructed and NCI-H1299 cells were divided into three groups: blank control group, negative control group and experimental group. The blank control group was the untreated cell group, while the negative control group was the lentivirus transfected cell group by the blank vector. The experimental group was the lentivirus transfected cell group constructed by the lentivirus interference vector of shRNA Twist. The siRNA interference expression vector of Twist was constructed by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot to detect the expression of Twist. Transwell kit was used to detect cell invasion. Cell counting kit-8 (CCK-8) kit was used to detect cell proliferation. Results: ⑴ The titer of lentivirus was detected. The transfection titer of lentivirus vector: shRNA-Twist vector was 3×10⁸ TU/ml. ⑵ The results of qRT-PCR test showed that compared with the negative control group, the mRNA expression of Twist in the experimental group was decreased (q=3.177, P=0.0234). ⑶ The results of Western blot showed that compared with the negative control group, the protein expression of Twist in the experimental group was decreased (q=4.071, P=0.0304). ⑷ The results of Transwell test showed that there was significant statistical difference among the three groups (F=19.472, P=0.000). Compared with the negative control group, the number of cell imigration in the experimental group was decreased (q=3.567, P=0.0318). ⑸ The results of CCK-8 showed that there was significant statistical difference among the three groups (F=20.983, P=0.000). Compared with the negative control group, the proliferation rate in the experimental group was decreased (q=5.272, P=0.0157). Conclusions ShRNA Twist gene can significantly inhibit the proliferation and invasion of lung adenocarcinoma NCI-H1299 cells.