Aged, 80 and over ; Exanthema ; etiology ; Female ; Humans ; Leukemia, Monocytic, Acute ; pathology ; Leukemic Infiltration ; complications ; diagnosis ; Skin ; pathology

Objective: To study the effect of orthodontic treatment on alveolar bone density and epidermal growth factor (EGF) expression in patients with periodontal disease. Methods : 50 patients with periodontal disease admitted into our hospital from January 2015 to January 2016 were selected as research object. Results : Before treatment, patients were given dental health instruction. Under the conditions of patients agreeing and cooperating, patients received orthodontic treatment. The changes of alveolar bone density, EGF in blood, saliva, gingival crevicular fluid (GCF), indexes of periodontal inflammation (gingival index, papilla Bleeding index, probing depth) of patients before and after treatment for one month were compared. Before treatment, the mandibular alveolar bone density was significantly higher than maxillary alveolar bone density (P < 0.05); After treatment, the maxillary and mandibular alveolar bone density were significantly lower than before treatment, and maxillary bone density was significantly lower than mandibular bone density (P < 0.05); After one-month treatment, the concentration of EGF expression in blood, saliva compared with before treatment, there was no statistical significance (P > 0.05); After one-month treatment, the concentration of EGF expression in GCF was significantly higher than before treatment (P < 0.05); Before and after treatment for one month, the concentration of EGF expression in GCF was significantly higher than blood, saliva (P < 0.05); After treatment, the GI, PBI, PD levels were significantly lower than before treatment (P < 0.05); After treatment, flow of saliva and GCF were significantly higher than before treatment (P < 0.05). Conclusion Orthodontic treatment has certain influence on EGF expression in GCF, but has no obvious influence on EGF expression in blood and saliva; The increase of EGF expression derives from periodontal tissue cells and has close association with periodontal remodeling, periodontal tissue cells can promote self-remodeling through autogenous regulation and local humoral regulation.

Humans ; Diverticulum, Colon ; Fecal Impaction ; Hydrodynamics

AIM:To study the curative efficacy of aspheric intraocular lens implantation after manual nuclear fragmentation in treatment of cataract and its effects on visual acuity.

METHODS: Totally 100 patients of cataract who received therapy from May 2013 to May 2016 in our hospital were selected. According to random number table, those patients were divided into the observation group(50 patients 68 eyes)and the control group(50 patients 67 eyes). The observation group was treated with small incision manual discectomy and aspheric intraocular lens implantation, the control group was treated with phacoemulsification cataract extraction and aspheric intraocular lens implantation, the operation effect of two groups were compared.

RESULTS: After operation, the corneal endothelial cell counts of the two groups decreased(P<0.05); in the observation group, the results were higher than those of the control group at 1 and 3mo after operation(P<0.05). In the observation group, the uncorrected visual acuity at 1mo after operation was better than that of the control group(P<0.05); there was no significant difference between the two groups in postoperative visual acuity after operation 3mo(P>0.05). At 1mo after surgery, the corneal astigmatism in the observation group was higher than that of the control group(P<0.05); there was no significant difference of corneal astigmatism between the two groups at 3mo after operation(P>0.05). The incidence of intraoperative complications and postoperative complications in the observation group were lower than that of the control group(P<0.05).

CONCLUSION: Aspheric intraocular lens implantation after manual nuclear fragmentation is well for cataract, which can reduce the damage to corneal endothelial cells, the complications rate is low and the postoperative visual acuity recovered well.

AIM: To observe the interaction of Ranibizumab and laser in the treatment of proliferative diabetic retinopathy(PDR).

METHODS: From December 2014 to August 2015, synchronous PDR patients with macular edema were divided into two groups. Thirty patients(48 eyes)were treated with Ranibizumab and laser, and 28 patients(45 eyes)were treated with laser alone. In the combined treatment group, FFA was reexamined 1wk after intravitreal injection of Ranibizumab. The patient then received panretinal photocoagulation(PRP). PRP was performed in the laser group alone. The best corrected visual acuity(BCVA)at 1, 4 and 8wk after treatment was observed and recorded. Macular thickness was measured by OCT.

RESULTS: The BCVA and macular thickness were significantly different between the two groups at 1, 4 and 8wk after treatment(P<0.05). In the laser alone group, the visual acuity decreased and the thickness of the macular thickness increased after 1wk. After treated 4 and 8wk, visual acuity improved, macular thickness decreased. After 1wk of intravitreal injection, FFA showed retinal leakage improvement in all eyes in the combined treatment group, with neovascularization retracted in varying degrees.

CONCLUSION:Ranibizumab combined with photocoagulation, can improve early vision, and reduce macular edema. Anti-VEGF therapy and photocoagulation may play a synergistic role to some extent. Drugs antagonized VEGF, improved macular edema and microvascular function. It can enhance the laser effect.Effective photocoagulation can further improve retinal ischemia and hypoxia, inhibit the excessive expression of VEGF.

Amyotrophic lateral sclerosis (ALS) is a progressively aggravating fatal neurodegenerative disease, and there is no radical cure. This paper reviews the current progress of ALS therapies, mainly including drug therapy, symptomatic management, stem cell transplantation and gene therapy.

Objective: To explore the mechanism of shRNA Twist gene on proliferation and invasion of lung adenocarcinoma NCI-H1299 cells. Methods: Twist siRNA interference expression vector was constructed and NCI-H1299 cells were divided into three groups: blank control group, negative control group and experimental group. The blank control group was the untreated cell group, while the negative control group was the lentivirus transfected cell group by the blank vector. The experimental group was the lentivirus transfected cell group constructed by the lentivirus interference vector of shRNA Twist. The siRNA interference expression vector of Twist was constructed by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot to detect the expression of Twist. Transwell kit was used to detect cell invasion. Cell counting kit-8 (CCK-8) kit was used to detect cell proliferation. Results: ⑴ The titer of lentivirus was detected. The transfection titer of lentivirus vector: shRNA-Twist vector was 3×10⁸ TU/ml. ⑵ The results of qRT-PCR test showed that compared with the negative control group, the mRNA expression of Twist in the experimental group was decreased (q=3.177, P=0.0234). ⑶ The results of Western blot showed that compared with the negative control group, the protein expression of Twist in the experimental group was decreased (q=4.071, P=0.0304). ⑷ The results of Transwell test showed that there was significant statistical difference among the three groups (F=19.472, P=0.000). Compared with the negative control group, the number of cell imigration in the experimental group was decreased (q=3.567, P=0.0318). ⑸ The results of CCK-8 showed that there was significant statistical difference among the three groups (F=20.983, P=0.000). Compared with the negative control group, the proliferation rate in the experimental group was decreased (q=5.272, P=0.0157). Conclusions ShRNA Twist gene can significantly inhibit the proliferation and invasion of lung adenocarcinoma NCI-H1299 cells.

Fulminant myocarditis is characterized by acute hemodynamic disorder, with rapid progress and high mortality.This article reviewed the current diagnostic criteria, the development of diagnostic techniques, as well as the development of current immunological, antiviral, and mechanical circulation adjuvant therapy for fulminant myocarditis in children.

ObjectiveTo compare the clinical effects of conventional versus laparoscopic surgery in adults with congenital bile duct dilatation. MethodsA retrospective analysis was performed on the clinical data of 37 patients with congenital bile duct dilation who were treated in our hospital from February 2011 to February 2013. All the 37 cases underwent bile duct cyst resection and common hepatic duct-jejunal Roux-en-Y anastomosis; 20 of them (study group) underwent laparoscopic surgery, while the other 17 cases (control group) underwent open surgery. The mean operative time, mean intraoperative blood loss, mean length of hospital stay after surgery, mean time to first flatus after surgery, and postoperative complications were compared between the two groups. Comparison of continuous data between the two groups was made by t test, while comparison of categorical data was made by chi-square test. ResultsCompared with the control group, the study group had a significantly longer operative time (P＜0.05) but significantly less mean intraoperative blood loss, mean length of hospital stay after surgery, and mean time to first flatus after surgery (P＜0.05). The incidence of postoperative complications showed no significant difference between the two groups (P＞0.05). ConclusionCompared with conventional surgery, laparoscopic bile duct cyst resection and common hepatic duct-jejunal Roux-en-Y anastomosis is less invasive and safer and leads to faster recovery in adults with congenital bile duct dilation, and it is worthy of clinical application.

ObjectiveTo investigate the short-term efficacy, long-term efficacy, and adverse effects of elemene combined with transcatheter arterial chemoembolization (TACE) in the treatment of primary liver cancer (PLC). MethodsPubMed, EMBASE, Cochrane Library, Chinese Scientific Journal Full-Text Database, Wanfang Data, CBM, and VIP were searched by two reviewers using the same search strategy for clinical studies on elemene combined with TACE in the treatment of PLC. The Jadad quality scoring system was used to assess the quality of the studies included, and Review Manager 5.2 software was used for the meta-analysis. ResultsA total of 9 articles involving 487 patients were included. The results of the meta-analysis showed that compared with the TACE group, the elemene-TACE group showed significant increases in the response rate, disease control rate, and 12- and 24-month survival rates (response rate: RR=1.43, 95%CI： 1.23-1.67, P＜0.001; disease control rate: RR=1.22, 95%CI: 1.11-1.32, P＜0.001; 12-month survival rate: RR=1.68, 95%CI: 1.22-2.31, P=0.001; 24-month survival rate: RR=2.91, 95%CI: 1.44-5.87, P=0.003). As for the incidence of adverse events, the elemene-TACE group showed a significant reduction in the incidence rate of abdominal pain (RR=0.59, 95%CI: 0.36-0.98, P=0.04). ConclusionCompared with TACE alone, elemene combined with TACE might improve response rate, disease control rate, and survival rates and reduce adverse events in patients with liver cancer.