2.A Case of Giant Coronary Artery Aneurysm in the Right Atrium Associated with a Right Coronary-Right Atrial Fistula
Motohiro Oshiumi ; Kazuhiro Hashimoto ; Hiroshi Okuyama ; Ryuichi Nagahori ; Gen Shinohara ; Masamichi Nakano
Japanese Journal of Cardiovascular Surgery 2004;33(5):352-355
Coronary artery fistula is an unusual congenital anomaly, particularly in association with coronary aneurysm. In the present case, a right coronary fistula leading to the right atrium was associated with a giant coronary aneurysm. There have only been 3 such cases reported in the literature. Since both the aneurysm and the fistula were completely thrombosed, no heart murmur was detected and the patient was initially diagnosed as having an intracardiac tumor by echocardiography.
3.Rapid Identification of bla(IMP-1) and bla(IMP-6) by Multiplex Amplification Refractory Mutation System PCR.
Akiyo NAKANO ; Ryuichi NAKANO ; Yuki SUZUKI ; Kyoichi SAITO ; Kei KASAHARA ; Shiro ENDO ; Hisakazu YANO
Annals of Laboratory Medicine 2018;38(4):378-380
No abstract available.
Polymerase Chain Reaction*
4.Polymicrobial Anaerobic Bacteremia Caused by Butyricimonas virosa and Brachyspira pilosicoli in a Patient with Peritonitis following Intestinal Perforation
Yoshihiko OGAWA ; Masatoshi SATO ; Takaya YAMASHITA ; Ryuichi NAKANO ; Satoshi MOCHIZUKI ; Kei KASAHARA ; Hisakazu YANO ; Keiichi MIKASA
Annals of Laboratory Medicine 2018;38(1):71-73
No abstract available.
Bacteremia
;
Brachyspira
;
Humans
;
Intestinal Perforation
;
Peritonitis
5.A Novel Mismatched PCR-Restriction Fragment Length Polymorphism Assay for Rapid Detection of gyrA and parC Mutations Associated With Fluoroquinolone Resistance in Acinetobacter baumannii
Naoki KAKUTA ; Ryuichi NAKANO ; Akiyo NAKANO ; Yuki SUZUKI ; Ayako TANOUCHI ; Takashi MASUI ; Saori HORIUCHI ; Shiro ENDO ; Risako KAKUTA ; Yasuo ONO ; Hisakazu YANO
Annals of Laboratory Medicine 2020;40(1):27-32
BACKGROUND:
Mutations in the quinolone resistance-determining regions (QRDRs) of Acinetobacter baumannii DNA gyrase (gyrA) and topoisomerase IV (parC) are linked to fluoroquinolone (FQ) resistance. We developed a mismatched PCR-restriction fragment length polymorphism (RFLP) assay to detect mutations in the gyrA and parC QRDRs associated with FQ resistance in A. baumannii.
METHODS:
Based on the conserved sequences of A. baumannii gyrA and parC, two primer sets were designed for mismatched PCR-RFLP to detect mutations in gyrA (codons 83 and 87) and parC (codons 80 and 84) by introducing an artificial restriction enzyme cleavage site into the PCR products. This assay was evaluated using 58 A. baumannii strains and 37 other Acinetobacter strains that have been identified by RNA polymerase β-subunit gene sequence analysis.
RESULTS:
PCR amplification of gyrA and parC was successful for all A. baumannii strains. In 11 FQ -susceptible strains, the gyrA and parC PCR products were digested by the selected restriction enzymes at the site containing gyrA (codons 83 and 87) and parC (codons 80 and 84). PCR products from 47 FQ-resistant strains containing mutations in gyrA and parC were not digested by the restriction enzymes at the site containing the mutation. As for the non-baumannii Acinetobacter strains, although amplification products for gyrA were obtained for 28 strains, no parC amplification product was obtained for any strain.
CONCLUSIONS
This assay specifically amplified gyrA and parC from A. baumannii and detected A. baumannii gyrA and parC mutations with FQ resistance.
6.First Two Cases of Infected Aortic Aneurysm Caused by Non-Vaccine Streptococcus pneumoniae Serotype 23A
Risako KAKUTA ; Ryuichi NAKANO ; Hisakazu YANO ; Daiki OZAWA ; Nobuo OHTA ; Takayuki MATSUOKA ; Naotaka MOTOYOSHI ; Shunsuke KAWAMOTO ; Yoshikatsu SAIKI ; Yukio KATORI ; Mitsuo KAKU
Annals of Laboratory Medicine 2020;40(3):270-273
7.The Details of Inpatient Cancer Rehabilitation Provided by Designated Cancer Hospitals in Japan
Takuya FUKUSHIMA ; Tetsuya TSUJI ; Jiro NAKANO ; Shun ISHII ; Shinsuke SUGIHARA ; Hiroshi SATO ; Juichi KAWAKAMI ; Hitoshi KAGAYA ; Akira TANUMA ; Ryuichi SEKINE ; Keita MORI ; Sadamoto ZENDA ; Akira KAWAI
Palliative Care Research 2023;18(2):143-152
Objective: This study aimed to clarify the details of inpatient cancer rehabilitation interventions provided by designated cancer hospitals in Japan. Methods: This questionnaire-based survey asked specialists regarding the outline of their facilities’ inpatient cancer rehabilitation, Dietz classification, disease, and intervention details. Results: Restorative interventions were the most common, and the most common cancer was lung cancer followed by colorectal cancer; hematologic malignancy; gastric cancer; and liver, gallbladder, and pancreatic cancer. Intervention proportions for colorectal and gastric cancer were significantly higher in general hospitals than in university hospitals and cancer centers; in contrast, those for hematological malignancy were significantly higher in university hospitals than in general hospitals. For bone and soft tissue sarcomas, intervention proportions in cancer centers were significantly higher than those in university and general hospitals; and for oral, pharyngeal, and laryngeal cancers, they were significantly higher in university hospitals and cancer centers than in general hospitals. The most common intervention was walking training, followed by resistance training, basic motor training, activities of daily living training, and respiratory rehabilitation. Respiratory rehabilitation was performed significantly more frequently in university and general hospitals than in cancer centers.Conclusion: The diseases had differed according to the characteristics of the facilities, and the interventions were considered accordingly. In future, it will be necessary to verify the effectiveness of inpatient cancer rehabilitation according to facility characteristics and to disseminate information on inpatient cancer rehabilitation.