We used a scanning electron microscope for identification of the two ticks which were found parasitic on two women.
(1) The patients were women of 69 and 62 years old.The main complaints were a tumour-formation on the skin of the upper abdominal region with itching in one case and the pain at the cicatrice of a past operation of the chest in another.In both cases a purulent inflamation appeared after 7 to 10 days at the site of bite and healed after 10 to 14 days with pigmentation.
(2) In our study through a scanning electron microscope we examined the morphological characters of marginal body setae, porose area, internal as well as external spurs of coxa I, and genital pore. The scanning electron microscope was effective especially to observe marginal body setae, spiracular plate, and internal as well as external spurs of coxa I, which are important for discriminating Ixodes nipponensis from Ixodes persulcatus.
We would expect that the morphological and physiological study of Ixodes species will develop hereafter concurrently with the parasitism of man by Ixodes nipponensis from the epidemiological standpoint.

Striated muscle insertions into the skin and mucosa are present in the head, neck, and pelvic floor. We reexamined the histology of these tissues to elucidate their role in transmission of the force. We examined histological sections of 25 human fetuses (gestational ages of ~11–19 weeks and ~26–40 weeks) and 6 cadavers of elderly individuals. Facial muscle insertion or terminal almost always formed as an interdigitation with another muscle or as a circular arrangement in which muscle fiber insertions were sandwiched and mechanically supported by other muscle fibers (like an in-series muscle). Our examination of the face revealed some limited exceptions in which muscle fibers that approached the dermis were always in the nasalis and mentalis muscles, and often in the levator labii superioris alaeque nasi muscle. The buccinator muscle was consistently inserted into the basement membrane of the oral mucosa. Parts of the uvulae muscle in the soft palate and of the intrinsic vertical muscle of the tongue were likely to direct toward the mucosa. In contrast, the pelvic floor did not contain striated muscle fibers that were directed toward the skin or mucosa. Although ‘cutaneous muscle’ is a common term, the actual insertion of a muscle into the skin or mucosa seemed to be very rare. Instead, superficial muscle insertion often consisted of interdigitated muscle bundles that had different functional vectors. In this case, the terminal of one muscle bundle was sandwiched and fixed mechanically by other bundles.