1.Inhibitory effect of Mg2+ on the release of Ca2+ from Ryanodine receptor of the sarcoplasmic reticulum in the skeletal muscle.
The Korean Journal of Thoracic and Cardiovascular Surgery 1992;25(4):347-355
No abstract available.
Muscle, Skeletal*
;
Ryanodine Receptor Calcium Release Channel*
;
Ryanodine*
;
Sarcoplasmic Reticulum*
3.Ca(2+)-induced Ca2+ Release from Internal Stores in INS-1 Rat Insulinoma Cells.
Kyung Jin CHOI ; Dong Su CHO ; Ju Young KIM ; Byung Joon KIM ; Kyung Moo LEE ; Shin Hye KIM ; Dong Kwan KIM ; Se Hoon KIM ; Hyung Seo PARK
The Korean Journal of Physiology and Pharmacology 2011;15(1):53-59
The secretion of insulin from pancreatic beta-cells is triggered by the influx of Ca2+ through voltage-dependent Ca2+ channels. The resulting elevation of intracellular calcium ([Ca2+]i) triggers additional Ca2+ release from internal stores. Less well understood are the mechanisms involved in Ca2+ mobilization from internal stores after activation of Ca2+ influx. The mobilization process is known as calcium-induced calcium release (CICR). In this study, our goal was to investigate the existence of and the role of caffeine-sensitive ryanodine receptors (RyRs) in a rat pancreatic beta-cell line, INS-1 cells. To measure cytosolic and stored Ca2+, respectively, cultured INS-1 cells were loaded with fura-2/AM or furaptra/AM. [Ca2+]i was repetitively increased by caffeine stimulation in normal Ca2+ buffer. However, peak [Ca2+]i was only observed after the first caffeine stimulation in Ca2+ free buffer and this increase was markedly blocked by ruthenium red, a RyR blocker. KCl-induced elevations in [Ca2+]i were reduced by pretreatment with ruthenium red, as well as by depletion of internal Ca2+ stores using cyclopiazonic acid (CPA) or caffeine. Caffeine-induced Ca2+ mobilization ceased after the internal stores were depleted by carbamylcholine (CCh) or CPA. In permeabilized INS-1 cells, Ca2+ release from internal stores was activated by caffeine, Ca2+, or ryanodine. Furthermore, ruthenium red completely blocked the CICR response in permeabilized cells. RyRs were widely distributed throughout the intracellular compartment of INS-1 cells. These results suggest that caffeine-sensitive RyRs exist and modulate the CICR response from internal stores in INS-1 pancreatic beta-cells.
Animals
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Caffeine
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Calcium
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Carbachol
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Cytosol
;
Indoles
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Insulin
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Insulinoma
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Rats
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Ruthenium Red
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Ryanodine
;
Ryanodine Receptor Calcium Release Channel
4.Na+-Ca2+ Exchange Curtails Ca2+ before Its Diffusion to Global Ca2+i in the Rat Ventricular Myocyte.
The Korean Journal of Physiology and Pharmacology 2005;9(2):95-101
In the heart, Na+-Ca2+ exchange (NCX) is the major Ca2+ extrusion mechanism. NCX has been considered as a relaxation mechanism, as it reduces global [Ca2+]i raised during activation. However, if NCX locates in the close proximity to the ryanodine receptor, then NCX would curtail Ca2+ before its diffusion to global Ca2+i. This will result in a global [Ca2+]i decrease especially during its ascending phase rather than descending phase. Therefore, NCX would decrease the myocardial contractility rather than inducing relaxation in the heart. This possibility was examined in this study by comparing NCX-induced extrusion of Ca2+ after its release from SR in the presence and absence of global Ca2+i transient in the isolated single rat ventricular myocytes by using patch-clamp technique in a whole-cell configuration. Global Ca2+i transient was controlled by an internal dialysis with different concentrations of BAPTA added in the pipette. During stimulation with a ramp pulse from +100 mV to -100 mV for 200 ms, global Ca2+i transient was suppressed only mildly, and completely at 1 mmol/L, and 10 mmol/L BAPTA, respectively. In these situations, ryanodine-sensitive inward NCX current was compared using 100micromol/L ryanodine, Na+ depletion, 5 mmol/L NiCl2 and 1micromol/L nifedipine. Surprisingly, the result showed that the ryanodine-sensitive inward NCX current was well preserved after 10 mmol/L BAPTA to 91 % of that obtained after 1 mmol/L BAPTA. From this result, it is concluded that most of the NCX-induced Ca2+ extrusion occurs before the Ca2+ diffuses to global Ca2+i in the rat ventricular myocyte.
Animals
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Architectural Accessibility
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Dialysis
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Diffusion*
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Heart
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Muscle Cells*
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Nifedipine
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Patch-Clamp Techniques
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Rats*
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Relaxation
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Ryanodine
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Ryanodine Receptor Calcium Release Channel
5.A Double Mutation of the Ryanodine Receptor Type 1 Gene in a Malignant Hyperthermia Family with Multiminicore Myopathy.
Seul Ki JEONG ; Dong Chan KIM ; Yong Gon CHO ; Il Nam SUNWO ; Dal Sik KIM
Journal of Clinical Neurology 2008;4(3):123-130
BACKGROUND AND PURPOSE: At least 100 Ryanodine receptor type 1 (RYR1) mutations associated with malignant hyperthermia (MH) and central core disease (CCD) have been identified, but 2 RYR1 mutations accompanying multiminicore myopathy in an MH and/or CCD family have been reported only rarely. METHODS: Fifty-three members of a large MH family were investigated with clinical, histopathologic, RYR1 mutation, and haplotyping studies. Blood creatine kinase (CK) and myoglobin levels were also measured where possible. RESULTS: Sequencing of the entire RYR1 coding region identified a double RYR1 mutation (R2435H and A4295V) in MH/CCD regions 2 and 3. Haplotyping analysis revealed that the two missense heterozygous mutations (c.7304G>A and c.12891C>T) were always present on a common haplotype allele, and were closely cosegregated with histological multiminicores and elevated serum CK. All the subjects with the double mutation showed elevated serum CK and myoglobin, and the obtained muscle biopsy samples showed multiminicore lesions, but only two family members presented a late-onset, slowly progressive myopathy. CONCLUSIONS: We found multiminicore myopathy with clinical and histological variability in a large MH family with an unusual double RYR1 mutation, including a typical CCD-causing known mutant. These results suggest that multiminicore lesions are associated with the presence of more than two mutations in the RYR1 gene.
Alleles
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Biopsy
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Clinical Coding
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Creatine Kinase
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Haplotypes
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Humans
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Malignant Hyperthermia
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Muscles
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Muscular Diseases
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Myoglobin
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Myopathies, Structural, Congenital
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Myopathy, Central Core
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Ophthalmoplegia
;
Ryanodine
;
Ryanodine Receptor Calcium Release Channel
6.Ryanodine Receptor-mediated Calcium Release Regulates Neuronal Excitability in Rat Spinal Substantia Gelatinosa Neurons.
International Journal of Oral Biology 2015;40(4):211-216
Nitric Oxide (NO) is an important signaling molecule in the nociceptive process. Our previous study suggested that high concentrations of sodium nitroprusside (SNP), a NO donor, induce a membrane hyperpolarization and outward current through large conductances calcium-activated potassium (BKca) channels in substantia gelatinosa (SG) neurons. In this study, patch clamp recording in spinal slices was used to investigate the sources of Ca2+ that induces Ca2+-activated potassium currents. Application of SNP induced a membrane hyperpolarization, which was significantly inhibited by hemoglobin and 2-(4-carboxyphenyl) -4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide potassium salt (c-PTIO), NO scavengers. SNP-induced hyperpolarization was decreased in the presence of charybdotoxin, a selective BKCa channel blocker. In addition, SNP-induced response was significantly blocked by pretreatment of thapsigargin which can remove Ca2+ in endoplasmic reticulum, and decreased by pretreatment of dentrolene, a ryanodine receptors (RyR) blocker. These data suggested that NO induces a membrane hyperpolarization through BKca channels, which are activated by intracellular Ca2+ increase via activation of RyR of Ca2+ stores.
Animals
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Calcium*
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Charybdotoxin
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Endoplasmic Reticulum
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Humans
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Membranes
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Neurons*
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Nitric Oxide
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Nitroprusside
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Potassium
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Rats*
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Ryanodine Receptor Calcium Release Channel
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Ryanodine*
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Substantia Gelatinosa*
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Thapsigargin
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Tissue Donors
7.The effects of local anesthetics on intracellular Ca2+ release from ryanodine-sensitive Ca2+ stores in gerbil hippocampal neurons.
Junfeng CHEN ; Weiren XU ; Hao JIANG
Chinese Medical Journal 2002;115(10):1542-1544
OBJECTIVETo examine the effects of procaine and lidocaine on intracellular Ca(2+) release from sarcoplasmic reticulum ryanodine-sensitive Ca(2+) stores.
METHODSThe experiment was performed on hippocampal slices from 60-80 g male Mongolian gerbils. Levels of intracellular Ca(2+) concentration in the slices were measured by microfluorometry. The slices were perfused with 50 mmol/L KCl containing medium for 30 seconds. Then, the medium was switched to physiological medium. After 5 min of incubation, the slice was perfused with 20 mmol/L caffeine containing physiology medium for 2 min. Following incubation, the slice was superfused with physiological medium until the end of the experiment. The effects of procaine and lidocanin (100 micro mol/L) on caffeine-evoked Ca(2+) release were evaluated by adding them to the medium after high K(+) medium perfusion.
RESULTSCaffeine induced a marked increase in intracellular Ca(2+) concentration which was then decreased 12% upon the addition of procaine (P < 0.05); however, lidocaine, did not induce a similar inhibitory reaction.
CONCLUSIONProcaine inhibits ryanodine-receptor mediated Ca(2+) release from intracellular Ca(2+) stores, while lidocaine may inhibit Ca(2+) release through other mechanisms.
Anesthetics, Local ; pharmacology ; Animals ; Calcium ; metabolism ; Gerbillinae ; Hippocampus ; drug effects ; metabolism ; Lidocaine ; pharmacology ; Male ; Procaine ; pharmacology ; Ryanodine ; pharmacology ; Ryanodine Receptor Calcium Release Channel ; physiology
8.Comparison of ryanodine binding to cardiac sarcoplasmic reticulum and nuclear envelope of rat.
Pei-Yong WANG ; Jun YANG ; Lin-Wang DONG ; Yong-Zheng PANG ; Chao-Shu TANG
Chinese Journal of Applied Physiology 2002;18(1):43-46
AIMThe characteristics of ryanodine receptor in rat cardiac sarcoplasmic reticulum (SR) and nuclear envelope (NE) were studied.
METHODSVelocity and isopyknic gradient centrifugation was employed to fractionate rat SR and NE. Ryanodine receptor was assayed with [3H] ryanodine saturate binding to the preparations.
RESULTSThe maximal binding (Bmax) and dissociating constant (Kd) of ryanodine receptor in rat cardiac NE were, 1.7% and 60% of those in SR respectively. Phosphorylation in vitro by PKA and PKC increased Bmax of the receptors in SR by 372% and 121%, and augmented those in NE by 221% and 306%, without any effects on Kd.
CONCLUSIONRyanodine receptors were present in rat myocardial NE, with lower density and higher affinity than those located in SR, which can be activated by PKA and PKC.
Animals ; Calcium ; metabolism ; Kinetics ; Myocardium ; metabolism ; Nuclear Envelope ; metabolism ; physiology ; Phosphorylation ; Rats ; Rats, Sprague-Dawley ; Ryanodine ; metabolism ; Ryanodine Receptor Calcium Release Channel ; metabolism ; Sarcoplasmic Reticulum ; metabolism ; physiology
9.Thyroid hormone-induced alterations of ryanodine and dihydropyridine receptor protein expression in rat hear.
Hae Won KIM ; Miyoung PARK ; Eun Hee LEE ; Hyoung Jin CHO ; Hee Ran LEE
The Korean Journal of Physiology and Pharmacology 1999;3(3):329-337
Thyroid hormone-induced cellular dysfunctions may be associated with changes in the intracellular Ca2+ concentration. The ryanodine receptor, a Ca2+ release channel of the SR, is responsible for the rapid release of Ca2+ that activates cardiac muscle contraction. In the excitation-contaction coupling cascade, activation of ryanodine receptors is initiated by the activity of sarcolemmal Ca2+ channels, the dihydropyridine receptors. In hyperthyroidism left ventricular contractility and relaxation velocity were increased, whereas these parameters were decreased in hypothyroidism. The mechanisms for these changes have been suggested to include alterations in the expression and/or activity levels of various proteins. In the present study, quantitative changes of ryanodine receptors and the dihydropyridine receptors, and the functional consequences of these changes in various thyroid states were investigated. In hyperthyroid hearts, (3H)ryanodine binding and ryanodine receptor mRNA levels were increased, but protein levels of ryanodine were not changed significantly. However, the above parameters were markedly decreased in hypothyroid hearts. In case of dihydropyridine receptor, there were a significant increase in the mRNA and protein levels, and (3H)nitrendipine binding, whereas no changes were observed in these parameters of hypothyroid hearts. Our findings indicate that hyperthyroidism is associated with increases in ryanodine receptor and dihydropyridine receptor expression levels, which is well correlated with the ryanodine and dihydropyridine binding. Whereas opposite changes occur in ryanodine receptor of the hypothyroid hearts.
Animals
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Calcium Channels, L-Type*
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Heart
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Hyperthyroidism
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Hypothyroidism
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Myocardium
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Rats*
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Relaxation
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RNA, Messenger
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Ryanodine Receptor Calcium Release Channel
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Ryanodine*
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Thyroid Gland*
10.Characteristics of Ca2+ stores in rabbit cerebral artery myocytes.
Sung Joon KIM ; Jin Kyung KIM ; Insuk SO ; Suk Hyo SUH ; Sang Jin LEE ; Ki Whan KIM
The Korean Journal of Physiology and Pharmacology 1998;2(3):313-322
In a myocyte freshly isolated from rabbit cerebral artery, the characteristics of Ca2+ release by histamine or caffeine were studied by microspectrofluorimetry using a Ca2+-binding fluorescent dye, fura-2. Histamine (5 micrometer) or caffeine (10 mM) induced a phasic rise of cytoplasmic free Ca2+ concentration ((Ca2+)c) which could occur repetitively with extracellular Ca2+ but only once or twice in Ca2+-free bathing solution. Also, the treatment with inhibitor of sarcoplasmic reticulum Ca2+-ATPase suppressed the rise of (Ca2+)c by histamine or caffeine. In Ca2+-free bathing solution, short application of caffeine in advance markedly attenuated the effect of histamine, and vice versa. In normal Ca2+-containing solution with ryanodine (2 micrometer), the caffeine-induced rise of (Ca2+)c occurred only once and in this condition, the response to histamine was also suppressed. On the other hand, in the presence of ryanodine, histamine could induce repetitive rise of (Ca2+)c while the amplitude of peak rise became stepwisely decreased and eventually disappeared. These results suggest that two different Ca2+-release mechanisms (caffeine-sensitive and histamine-sensitive) are present in rabbit cerebral artery myocyte and the corresponding pools overlap each other functionally. Increase of (Ca2+)c by histamine seems to partially activate ryanodine receptors present in caffeine-sensitive pool.
Baths
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Caffeine
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Cerebral Arteries*
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Cytoplasm
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Fura-2
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Hand
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Histamine
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Muscle Cells*
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Muscle, Smooth
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Ryanodine
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Ryanodine Receptor Calcium Release Channel
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Sarcoplasmic Reticulum