This paper is to report our study on the effects of indomethacin, fenbufen, ksto profen, naproxen and doxamethasone on the number of leucocytes, the phagocytosis of macrophages and the intracellucar cAMP level in the peptone induced peritoneal exudate of mouse and on the comparison of thses drugs with dexametha-sone.Under therapeutic dosage, indomethacin and ketoprofen could significantly decrease the number of leucocytes in the exudate (P

It was found that there was a significant increase of the number of circulating leucocytes 2h after a subcutaneous injcetion of isoprenaline and severe myocardial necrosis occurred after consecutive injections for 3 d.Cytoxan-induced depletion of leucocytes resulted in an obvious decrease of the percentage of myocardial necrosis.In the control.the neutrophilic phagocytosis was enhanced and their release of acid phosphatase elevated greatly,which indicates that the neutrophils are in an activated state.After the.neutrophils were inhibited with dexamethasone or indomethacint significant reduction of the contents of prostaglandin E2 and malondialdehyde and an increase of superoxide dismutase activty in the myocardium were observed and consequently myocardial necrosis was greatly alleviated.Our findings suggest that neutrophils might play an important role in the pathogenesis of myocardial infarction through the release of lysosomal enzymes and the production of oxygen-derived free radicals and certain metabolites of arachidonic acid,and proper regulation of the neutrophilic functions could exert prophylactic effects on myocardial infarction.This might be a promising direction to look for more effective drugs to treat myocardial infarction.

Twenty four hours after occluding of the middle cerebral artery in rats, thecirculating neutrophils were in activated state and the lipidperoxidation increased in ische-mic brain tissue. Tanshinone inhibited the chemoluminescence of the circulating neutrophilphagocytosis and decreased leukocyte infiltration in infarcted brain tissue. It alsodecreased lipoperoxide, malondialdehyde and increased SOD activity in ischemic cerebralregion. In addition, it reduced cerebral infarction size in the model. The correlationbetween inhibitory effects on leukocyte and reduction in ischemic cerebral damage wassignificant. Dexamethasone used as a positive control had similar effects on neutrophils andischemic cerebral injury. It was shown that the prophylactic effcts of Tanshinone oncerebral infarction might be mainly due to its inhibition on leukocyte function.

The relationship between the inhibitory effect of tetrandrine ( Tet ) on neutrophil ( Nen ) lysomal enzyme ( ?-glu-curonidasc , P-G ) release & calcium ion were studied. It was found that calcium ion added to the medium increased p-G release from Neu During incubation. After intracellular calcium was depleted by EGTA, P-G release was signifcantly decreased. While calcium was added, increase in P-G release reappeared. It indicated that both intracellular & extracellular calcium may be involved in P-G release. Tet & v erapamil ( Ver ) not only inhibited ?-G release induced either by calcium or serum opsonized zymOsan, but also inhibited potential operated channel of Neu. It was concludted that the inhibitory effect of Tet on Neu lysomal enzyme release was concerned with its calcium antagonism.

Objective To investigate the relative mechanism of Maimendong Tang (Ophiopogon Decoction) in amelioration of lung function and endoplasmic reticulum stress (ERS) in rats with bleomycin-induced pulmonary fibrosis.Methods Male healthy SD rats were randomly divided into normal group, model group, Maimendong Tang group 1 and Maimendong Tang group 2.The rat model of pulmonary fibrosis was established by bleomycin pulverization via tracheal intubation.On the 14 t h d after modeling, Maimendong Tang was intragastrically given to Maimendong Tang group1 and Maimendong Tang group 2 twice a day for 20 d continuously.The general conditions were observed, and pathological changes of lung tissue were observed after HE and Masson staining.The forced vital capacity (FVC) , 0.4 second rate (FEV0.4/FVC) , mass FVC, inspiratory resistance (Ri) , expiratory resistance (Re) and dynamic pulmonary compliance (Cydn) were detected by using spirometer.The expressions of glucose regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP) protein in lung tissue were analyzed by using Western blotting assay and immunohistochemistry staining technique.Results Compared with normal group, FVC, FVC/lung mass and Cydn decreased, and 0.4 second rate increased in model group.Compared with model group, FVC increased and 0.4 second rate decreased and Cydn increased in Maimendong Tang group 1 and Maimendong Tang group 2, and mass FVC increased in Maimendong Tang group 2.Pathological sections showed that there were chronic inflammatory cell infiltration in consolidation zone of lung tissue and massive collagen deposition, GRP78 and CHOP with high expressions were mainly positioned in vesicular type Ⅱ alveolar epithelial cells (AECⅡs).The inflammation was relieved, collagen deposition was decreased, protein expressions of GRP78 and CHOP were reduced and in Maimendong Tang group 2.Conclusion Maimendong Tang can significantly improve lung function and reduce lung interstitial collagen deposition, which may be related to regulation of protein expressions of GRP78 and CHOP in AECⅡs in consolidation zone of lung tissue, relief of ERS, and recovery of normal AECⅡs function.