Objective To explore the postoperative recovery of the patients with glioma-related epilepsy and the possible risk factors for recurrence of epilepsy.Methods To collect clinical data of 89 patients with glioma-related epilepsy,we recorded Engle grade score of all patients underwent resection of tumor and seizure focus in a week,1,3 and 6 month after surgery Repeated measures ANOVA was used to analysis the difference within group.Kaplan-Meier method and Cox regression analysis were used to analysis seizure recurrence risk curve and the epileptic recurrence related factors,respectively.Results The postoperative recurrence rate was 26.97％ (24/89).Engle grade scores were 2.966±0.081.2.202±0.080,1.730±0.093 and 1.313±0.042 in a week,1,3 and 6 month after surgery.The difference was statistically significant (Ftime=96.076,P=0.000).Cox regression model showed that tumor accumulation site (RR =2.908,CI:1.083 ～7.806),postoperative edema formation (RR =4.769,CI:1.737 ～ 13.096),tumor relapse (RR=8.309,CI:3.379～20.432)were the important risk factors for recurrence epileptic seizures (all P＜ 0.05).Conclusions Surgical treatment can significantly reduce or even eliminate seizures postoperative and its long term efficacy is superior to short term efficacy.Appropriate measures should be taken to treat risk factors associated with recurrence epileptiform seizure to improve the prognosis of patients quality of life.

Objective To explore the down-regulation of advanced receptor for advanced glycation end products(RAGE)on expression of high mobility group protein B1(HMGB1)in glioma cells line and the volume change of transplanted tumor in nude mice. Methods HMGB1 expression in glioma LN229 cells line (divided into a control group and a study group) was observed by immunohistochemical assay and Western blot. The control group received normal saline,whereas the study group received RAGE receptor blocking agent FPS-ZM1. Expression of HMGB1 protein was detected by the same methods. The difference of the expression was examined by independent sample t test. 30 Nu/Nu nude mice were randomly divided into two groups;the above two kinds cell lines were injected into the same area of the left back of nude mice. Six weeks after injection ,the volume size was measured six times ,and the variance of repeated measurement data was used to analyze the difference of the volume change. Results HMGB1 protein was mainly expressed in the cytoplasm and nucleus. As compared with the control group,HMGB1 protein expression levels were decreased in the study group(P < 0.05),the growth rate of transplanted tumor in nude mice was significantly faster in the control group than in the study group ,the difference was statistically significant(P < 0.05). Conclusions The growth and invasion of HMGB1 protein may be involved in glioma by RAGE receptor. RAGE receptor blocker FPS-ZM1 can significantly reduce the expression of HMGB1 protein and inhibit the growth of transplanted tumor volume. It is expected to be used for the research on glioma cell apoptosis.

Objective To explore the expression of human sodium coupled neutral amino acid transporter 1 (SNAT1) in human glioma tissues and its relationship with clinical pathological parameters and prognosis. Methods Immu?nohistochemical and western blotting were used to detect SNAT1 expression in glioma tissue and tumor peripheral tissue from 89 cases of glioma patients including 55 cases of low grade gliomas (WHO I-II), and 34 cases of high grade gliomas (WHO grade III-IV).χ2 test and was used to analyze the relationship between expression and clinical pathological param?eters of SNAT1. Kaplan-Meier method was used to analyze the effect of different expression of SNAT1 on the prognosis of patients and to establish the Cox regression model. Results The expression of SNAT1 was significantly higher in gliomas than in tumor peripheral tissue (t=-9.803, P=0.001). The expression of SNAT1 was significantly higher in high pathologi?cal grade tissues than in low grade of glioma tissues (t=-6.682, P=0.003). SNAT1 expression was associated with tumor di?ameter and pathological grade (χ2=4.963, 8.527, P<0.05);Cox regression model showed that the tumor pathological grade and different SNAT1 protein expression were independent risk factors for the prognosis of patients with glioma. Conclu?sions The expression of SNAT1 protein is closely associated with the pathological grade of gliomas and the prognosis of the patients, which may be a new target to judge the biological characteristics and to evaluate the prognosis of gliomas.

Objective:To explore the risk factors of diabetic nephropathy(DN).Methods:From January 2016 to September 2019, the clinical data of 403 patients with type 2 diabetes in the Integrated Traditional Chinese and Western Medicine Hospital of Wenzhou were analyzed retrospectively.Excluding other factors, they were divided into DM group (315 cases) and DN group(88 cases) according to whether or not they were complicated with DN.The age, course of disease, complications, monocyte to high-density lipoprotein cholesterol ratio(MHR) and other test data were included in the logistic regression model to analyze the independent risk factors of DN.Results:There were statistically significant differences in the course of ≥10 years[95 cases(30.16%) vs.37 cases(42.05%)], complicated with hypertension[141 cases(44.76%) vs.58 cases(65.91%)], insulin application[94 cases(29.84%) vs.39 cases(44.32%)], ACEI/ARB application[132 cases(41.90%) vs.62 cases(70.45%)], FBG[(7.38±2.45)mmol/L vs.(8.26±2.06)mmol/L], HbA1c≥7%[91 cases(28.89%) vs.36 cases(40.91%)], MHR[(10.30±4.38) vs.(17.10±7.18)] and HDL-C[(0.58±0.16)mmol/L vs.(0.52±0.17)mmol/L] between the two groups( t=4.41, 13.31, 6.52, 22.46, 3.08, 4.60, 11.02, 2.65, all P<0.05). Multivariate logistic regression analysis showed that hypertension( OR=2.06, 95% CI=1.36-2.76), HbA1c≥7%( OR=1.32, 95% CI=1.04-1.60), MHR≥11.78( OR=1.65, 95% CI=1.03-2.67) were independent risk factors for DN. Conclusion:Hypertension, HbA1c ≥7%, MHR ≥11.78 are independently correlated with DN, which provide a basis for clinical prognosis.

Objective:To explore the relationship between sex hormone binding globulin and glycolipid metabolism in patients with obese polycystic ovary syndrome.Methods:From April 2016 to April 2019, 114 patients with obese polycystic ovary syndrome in the Integrated Chinese and Western Medicine Hospital of Wenzhou were selected and divided into two groups according to the level of sex hormone binding globulin, including 66 cases in the low level group and 48 cases in the normal group.The blood glucose, blood lipid and sex hormone levels of the two groups were measured and the results were analyzed.Results:The levels of TC, TG, LDL-C and ApoB in the low level group[(2.32±0.97)mmol/L, (6.45±1.25)mmol/L, (3.57±1.01)mmol/L, (0.93±0.32)g/L] were significantly higher than those in the normal group[(1.87±0.89)mmol/L, (4.88±0.94)mmol/L, (3.12±0.88)mmol/L, (0.76±0.17)g/L]( t=2.477-7.322, all P<0.05). The level of ApoA in the low level group[(1.22±0.34)g/L] was significantly lower than that in the normal group[(1.58±0.47)g/L]( t=-4.748, P<0.05), while there was no statistically significant difference in HDL-C between the two groups[(1.45±0.31)mmol/L vs.(1.43±0.56)mmol/L, t=0.244, P>0.05]. The levels of FPG, HbA1C and FINS in the low level group[(5.53±1.45)mmol/L, (6.42±0.79)% and (18.71±5.46)mU/L] were significantly higher than those in the normal group[(5.09±0.76)mmol/L, (6.01±0.92)% and (15.49±4.98)mU/L]( t=2.136-3.225, all P<0.05), while there was no statistically significant difference in 2hPG between the two groups[(8.79±1.56)mmol/L vs.(8.32±1.84)mmol/L]( t=1.472, P>0.05). The incidence of insulin resistance in the low level group[54.55%(36/66)] was significantly higher than that in the normal group[35.42%(17/48)](χ 2=4.088, P<0.05). The level of T in the low level group[(61.45±21.44)ng/mL] was significantly higher than that in the normal group[(50.39±22.31)ng/mL]( t=2.673, P<0.05), while there were no statistically significant differences in FSH, LH, E 2 and PRL between the two groups( t=0.427-1.714, all P>0.05). Conclusion:The patients with low levels of sex hormone binding globulin are more likely to suffer from hyperglycemia and hyperlipidemia, and low levels of sex hormone binding globulin may be a risk factor for obese polycystic ovary syndrome.