Humans ; MicroRNAs ; genetics ; Rhinitis, Allergic ; genetics

Objective To study clinical efficacy of ulinastatin combined with naloxone in patients with cardiogenic shock(CS) after acute myocardial infarction (AMI).Methods Eighty patients with CS after AMI were randomly divided into routine treatment group (n =19),ulinastatin group (n =20),naloxone group (n =21) and ulinastatin combined with naloxone group (n =20).The levels of serum cardiac troponin I (cTnI),brain natriuretic peptide(BNP),tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6)were measured before and a week after treatment.In the meantime,recovery time of shock,the average hospitalization days and 28-day mortality rate were recorded.Results After the treatment,the levels of serum cTnI,BNP,TNF-α and IL-6decreased in all groups(P ＜ 0.01),and there was significant difference on the decreasing degree of cTnI,BNP,TNF-α and IL-6 in ulinastatin combined with naloxone group when compared with those in routine treatment group,ulinastatin group and naloxone group(cTnI:(1.04 ± 0.17) ng/L vs.(2.06 ± 0.15) ng/L,(1.59 ± 0.16)ng/L,(1.97 ± 0.14) ng/L; BNP:(143.21-56.94) ng/L vs.(261.07 ± 71.43) ng/L,(203.46 ± 65.73) ng/L,(252.96 ± 68.85) ng/L; TNF-α:(13.42 ± 8.93) ng/L vs.(31.21 ± 12.32) ng/L,(20.39 ± 11.08) ng/L,(28.98 ± 11.76) ng/L ; IL-6:(37.58 ± 11.14) ng/L vs.(80.46 ± 27.15) ng/L,(59.84 ± 20.72) ng/L,(76.15 ±26.45) ng/L; P ＜ 0.01).The recovery time of shock,the average hospitalization days and 28-day mortality rate in ulinastatin combined with naloxone group were significantly lower than those in routine treatment group,ulinastatin group and naloxone group(recovery time of shock:(7.16 ± 1.52) d vs.(11.43 ± 2.40) d,(8.05 ±1.81)d,(8.74 ± 1.98)d;the average hospitalization days:(15.03 ±3.23)d vs.(22.64 ±4.18)d,(18.93 ±3.97)d,(19.21 ±3.94)d ;28-day mortality rate:(41.62％ vs.61.20％,50.74％,52.31％ ; P ＜0.01)).Conclusion The application of ulinastatin combined with naloxone can effectively inhibit the cardiac injury and inflammatory response,promote the recovery of circulation function and improve prognosis in patients with CS after AMI.

Purpose: There is no appropriate instrument or devices and corresponding technological specification for measurement of output dose and dose disdribution in stereotactic radiothrapy. Small field of the stereotatic radiotherapy can't be measured by normal ionization chamber for its characteristics.A practible instrument and method were developed for dose measurement in stereotactic irradiation.Materials and Methods:A diode detector with 1mm sensitivety volume and a 0.3cc graphite chamber developed by us are used to measure the dose output & dose distribution in warious phantom for the beams used in stereotactic irradiation.Results:The dose outputs and dose ditributions measured by SCD-61 diode developed by us were tested and verified by TLD dosemeter,and the results show tha there is a 5% consistency for the value at the focus point.Conclusion: the diameter of the detector used for measurement must be smaller than the halt size of the radiation beams to be measured. A diode detector with 1mm sensitivity volume is the best choice for the dosimetric measurements for smaller beams used in stereotactic irradiation.

Objective To investigate the expression level and clinical significance of cyclinB1 in primary hepatocellular carcinoma(PHC).Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the mRNA level of cyclinB1 in liver cancer tissues and adjacent tissues of cancer.Results CyclinB1 mRNA was positive expression in 90%(36/40)cancer tissues and 85%(34/40)adjacent tissues of cancer.The expression level of cyclinB1 in cancer tissues (0.531?0.015)was significantly higher than that in adjacent tissues of cancer(0.263?0.023).The level of cyclinB1 was significantly associated with pathological grades and lymph node metastasis, but not associated with sex、age、tumor size or tumor thrombus.Conclusion CyclinB1 may play an important role in the tumorigenesis of PHC and may be a potential adjuvant parameter in evaluating pathological grades and lymph node metastasis.

0.05).Conclusion Cyclin B1 levels in liver cancer tissues were stronger than in adjacent cancer tissues and normal liver tissues.

Objective To investigate the p33~ ING1b mRNA level and the relationship to clinical pathological data in primary hepatocellular carcinoma(PHC).Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) method was employed to detect the mRNA level of p33~ ING1b in liver cancer tissues and adjacent tissues of cancer.Results p33~ ING1b mRNA was positive expression in 63.6% (35/55) cancer tissues and 70.9% (39/55) adjacent tissues of cancer.The expression level of p33~ ING1b mRNA in cancer tissues (0.410?0.175) was significantly lower than that in adjacent tissues of cancer (0.529?0.203). The level of p33~ ING1b was significantly associated with pathological grades, lymph node metastasis and envelope infiltration, but not associated with sex, age, tumor size, tumor thrombus or liver cirrhosis.Conclusion The decrease of p33~ ING1b may play an important role in the tumorigenesis and development of PHC, and may be an adjuvant parameter in evaluating tumor differentiation, invasion and prognosis.

Objective:To investigate the relation between eotaxin, STAT6 and allergic rhinitis through comparing expressions of eotaxin and STAT6 in nasal mucosa of normal guinea pigs and of guinea pig allergic rhinitis models. Methods: Twenty-four healthy guinea pigs were equally randomized into 2 groups: normal control and allergic rhinitis models. Guinea pig allergic rhinitis models were established by the ovalbumin challenge method. Protein expressions of eotaxin and STAT6 in the nasal mucosa of the 2 groups were detected by immunohistochemical technique. Results: Expressions of eotaxin and STAT6 were found in the nasal mucosa of both normal guinea pigs and allergic rhinitis guinea pigs. Eotaxin was mostly located in the cytoplasm of the serous and mucous glands,while STAT6 mostly in the cytoplasm of subepithelial infiltrative cells.Expressions of eotaxin and STAT6 in the nasal mucosa of allergic rhinitis guinea pigs were significantly higher than those of normal guinea pigs~(P

Objective: The activity of nitric oxide synthase(NOS)in nasal mucosa of normal guinea pigs was compared with that of allergic rhinitis(AR)guinea pigs to investigate the relationship between NOS and AR. Methods: Localization of NOS in nasal mucosa of normal and AR guinea pigs was examined by means of NADPH diaphorase (NADPH d) histochemical stain. Results: Nasal mucosa of both normal and AR groups was positive for NADPH d, though the AR group reacted more strongly. Reaction was localized to the cytoplasm of glandular cell, epithelium and vascular endothelium. Conclusion: There is distribution of NOS in nasal mucosa of normal guinea pigs. The activity of NOS in guinea pig AR model was significantly stronger than that in normal group. NOS is related to AR. [

Objective: To investigate the pathogenesis of allergic rhinitis at cell level by determing basophil histamine releasability in guinea pigs and attempt to find an objective way for the diagnosis of allergic rhinitis. Methods: A model of allergic rhinitis was established after healthy guinea pigs were randomly divided into 2 groups. The basophil histamine releasability to the stimulant of concanavalin A between normal and experimental guinea pigs was compared by means of fluorometric assay of histamine. Results: Basophil histamine release to various concentrations of concanavalin A in allergic rhinitis group was significantly enhanced as compared with that in normal control ( P

Objective: To explore the expression of Eotaxin and the effect of histamine in allergic rhinitis model (AR),and aim to explore the pathogenesis of AR. Method:The AR models were established by applicating of ovain albumin in rats. The expression of Eotaxin in nosal mucosa,serum and nasal cavity lavage fluid,were observed before and after treatment of histamine or its antagonist by immunochemistry,RT-PCR and ELISA technique. Result:The expression of Eotaxin in nasal lavage fluid and nasal mueosa increased after treatment of histamine(P＜0.05). Contrarily,the expression of Eotaxin in nasal lavage fluid,nasal mucosa and serum decreased after treatment of the antagonist of histamine. Conclusion:Both histamine and its receptor can involve in the pathogenesis of AR by affecting the expression of Eotaxin.