To study the growth, expansion and differentiation of bone marrow stromal cells (BMSC) of rhesus monkey, the BMSC were isolated from adult rhesus monkeys, cultured and induced with a special medium confected in our lab. Identification was performed with immunochemichemistry method. The results showed that BMSC could proliferate and generate clone when culturing in vitro. These cells grow rapidly and differentiated into neuron like cells and astrocyte like cells. The cell clones proliferated from stem cell could express Nestin antigen and the differentiated cells expressing GFAP or NSE antigen respectively. In this study, we did not observe RA, BFGF and EGF remarkably influencing the induction and differentiation of the BMSC. It is suggested that the BMSC from rhesus monkey have the self renewal and differentiation abilities. They might differentiate into neuron like and astrocyte like cells which express GFAP or NSE. As available easily, the BMSC could be considered as the ideal seed cells of the neural stem cells.

Objective: To establish the culture system of hairy root of Isatis indigotica and to induce the regeneration plant of hairy root. Methods: Hairy root of Isatis indigotica was obtained from infected cotyledon explants after infection with Agrobacterium rhizogenes strains A4, R1601 and ATCC15834. The better lines were selected. The growth curve was surveyed and extrinsic factors affecting the growth of hairy roots were investigated. The regeneration plant was induced on MS media with different hormones. The transformation of Ri T DNA was examined through high voltage paper electrophoresis. Results: The hairy root was originally obtained from Isatis indigotica . The regeneration plant was induced on MS media with BA. The result of high voltage paper electrophoresis confirmed the transformation of T DNA from Ri plasmid to the hairy root and regeneration plant. Conclusion: The acquisition of hairy root of Isatis indigotica and regeneration plant of it lay a foundation for the production of active component and introduction of foreign gene. [

Objective To investigate the timeliness and accuracy of collection of clinical urine specimen.Methods Totals of 371 collections of urine specimen were followed , the cause of not-in-time and inaccuracy was analyzed , improvement strategies were proposed , and effective collections of clinical specimen were managed .Results Totals of 330 urine specimens were collected timely and accurately , accounting for 88.95%, and 41 specimens failed, accounting for 11.05%, in which 10 were delayed because of patients'menstruation, anuria or inspection on that day (2.70%), and 31 were not collected timely or accurately (8.35%).Twenty-two urine specimens were wrongly collected because of the first urine specimen of admission (91.67%), ten were wrongly collected for leaving two or more than two urine specimens at the same time (41.67%), and twelve were wrongly collected because of family assistance due to low education level (50.00%).Conclusions The timeliness and accuracy should be listed as one of the quality monitoring indicators of specialist nurses .Nurses'knowledge and quality consciousness of specimens collection should be strengthened to help patients with collection , so as to improve the timeliness and accuracy of urine specimens collection.

Objective:To build an evaluation index system for the post competency of nurses in Kidney Intensive Care Unit (KICU) , so as to provide references for the post access management and training system setting of KICU nurses.Methods:Through literature review and semi-structured interview, the post competency evaluation index system of nurses in KICU was preliminarily constructed. Using the purposive sampling method, a total of 15 nursing experts were selected for 2 rounds of expert letter consultation from March to June 2021, and the indicators at all levels were screened, revised and improved. The evaluation index system for the post competency of nurses in KICU was established.Results:The effective recovery rates of the two rounds of questionnaires were both 100.00% (15/15) , the expert authority coefficients were respectively 0.85 and 0.87 and the Kendall harmony coefficients of total indicators were respectively 0.332 and 0.341 ( P<0.01) . The final evaluation index system of post competency of nurse in KICU included 4 first-level indicators, 16 second-level indicators and 51 third-level indicators. Conclusions:The constructed evaluation index system of post competency for KICU nurses is scientific and reliable, which can provide references for the post access management and training system setting of KICU nurses.