1.Effect of high-concentration hydrogen inhalation on sepsis-associated encephalopathy in mice
Ruqing ZHU ; Xing MAO ; Lina ZHENG ; Yonghao YU ; Dai SHEN ; Keliang XIE
Chinese Journal of Anesthesiology 2022;42(8):961-964
Objective:To evaluate the effect of high-concentration hydrogen inhalation on sepsis-associated encephalopathy (SAE) in mice.Methods:Healthy male ICR mice, aged 6-8 weeks, weighing 20-25 g, were divided into 4 groups ( n=50 each) using the random number table method: sham operation group (Sham group), SAE group, sham operation plus high-concentration hydrogen group (Sham+ H 2 group), and SAE plus high-concentration hydrogen group (SAE+ H 2 group). SAE model was prepared by cecal ligation and puncture (CLP) in anesthetized animals.At 1 and 6 h after operation, Sham+ H 2 and SAE+ H 2 groups inhaled the mixture of hydrogen and oxygen (67% hydrogen-33% oxygen) for 1 h, and Sham and SAE groups inhaled the mixture of nitrogen and oxygen (67% nitrogen-33% oxygen) for 1 h. The postoperative 7-day survival rate was recorded.Cognitive function was assessed by Y maze test at days 3, 5 and 7 after operation.The mice were sacrificed at 24 h after operation, and hippocampal tissues were obtained for microscopic examination of the pathological changes of neurons in hippocampal CA1 region (with a light microscope) and for determination of normal neuron count, contents of tumor necrosis factor-ɑ (TNF-α) and high mobility group box-1 (HMGB1) (by enzyme-linked immunosorbent assay), mitochondrial membrane potential (MMP) (by fluorescence spectrophotometry) and content of mitochondrial ATP (by fluorescein-fluorescent enzyme luminescence method). Results:Compared with Sham group, the 7-day survival rate after operation, percentage of spontaneous alternation at each time point after operation, and the number of normal neurons were significantly decreased, the contents of TNF-ɑ and HMGB1 were increased, and the contents of ATP and MMP were decreased in SAE and SAE+ H 2 groups ( P<0.05), and no significant change was found in Sham+ H 2 group ( P>0.05). Compared with SAE group, the 7-day survival rate after operation, percentage of spontaneous alternation at each time point after operation, and the number of normal neurons were significantly increased, the contents of TNF-ɑ and HMGB1 were decreased, and the contents of ATP and MMP were increased in SAE+ H 2 group ( P<0.05). Conclusions:High-concentration hydrogen inhalation can reduce SAE, and the mechanism may be related to reduction of hippocampal inflammatory responses and improvement in mitochondrial function in mice.
2.Perioperative nursing care of a child with Berry syndrome recognized by radical surgery
Ruqing XU ; Jihua ZHU ; Jun ZHOU ; Shanshan XIA ; Hui LI ; Yaping DING
Chinese Journal of Nursing 2023;58(23):2910-2914
To summarize the perioperative nursing experience of a child with Berry syndrome who underwent radical surgery.The key points of preoperative care include the prevention of heart failure and vigilance against ischemia and hypoxia in the lower limbs.The key points of postoperative care include maintaining stable cardiac function,preventing pulmonary hypertension crisis,ensuring safe transition during delayed chest closure,paying attention to psychological support,and emphasizing continuous care.After 54 days of careful treatment and nursing care,the child recovered well and was discharged from the hospital.The follow-up was couducted a month after discharge,and the child was good.