Objective To explore the correlation between TCM constitution and sleep status in escort troops. Methods Standardized TCM Constitution Questionnaire and Pittsburgh Sleep Quality Index (PSQI) were used to investigate escort troops. Factors of PSQI and constitution types were analyzed. Results The results of PSQI showed the number of escort troops who had bad sleeping (PSQI>7) was 64, which accounted for 46%of the escort troops, among which, 45 people (70.3%) were justified as biased constitution, while 19 were normal constitution (29.7%). The top three biased constitution in bad sleeping were:qi deficiency, phlegm-dampness and dampness-heat. PSQI factors such as sleep disorder, daytime dysfunction and total score had positive correlation with qi deficiency, yang deficiency, yin deficiency, dampness-heat and blood stasis, and negative correlation with normal constitution. Conclusion Sleep condition of escort troops has correlation with TCM constitution, which indicates that sleep status could be improved by regulating TCM constitution.

Objective To explore the diagnostic and prognostic value of combined detection of serum CC chemokine ligand 11(CCL11)and interferon regulatory factor 5(IRF5)levels for neonatal necrotizing enterocolitis(NEC).Methods A total of 115 children with NEC who visited Zhangjiakou Maternal and Child Health Hospital from August 2020 to May 2023 were collected as the study group,and 92 healthy newborns during the same period were selected as the control group.After 30 days of treatment,these patients were grouped into a survival group(n=89)and a death group(n=26)based on their survival status.Enzyme-linked immunosorbent assay(ELISA)method was applied to detect the levels of serum CCL11 and IRF5 in each group.Clinical data of children in the survival and death groups were collected and compared,and multivariate Logistic regression was applied to analyze the influencing factors of neonatal NEC occurrence.Receiver operating characteristic(ROC)curve was plotted to evaluate the diagnostic and prognostic value of serum CCL11 and IRF5 levels for neonatal NEC.Results Compared with the control group,the serum CCL11 level(1.41±0.62 pg/ml vs 0.79±0.28 pg/ml)and IRF5 level(5.34±2.16 pg/ml vs 3.29±1.43 pg/ml)in the study group were increased,and the differences were significant(t=8.891,7.831,all P＜0.001).The area under the curve(AUC)of the combined diagnosis of two for neonatal NEC was 0.898,with a sensitivity of 86.96％,and the combination of the two was superior to the diagnosis of CCL11 and IRF5 alone(Z=2.747,2.921,P=0.006,0.004).Compared with the survival group,the percentage of children with NEC in the death group with Bell stage Ⅱ～Ⅲ(76.92％vs 42.70％),the percentage of children with feeding mode of formula feeding(61.54％vs 24.72％),percentage with respiratory failure(34.62％vs 13.48％),treatment by surgery(53.85％vs 38.09％)were higher(x2=9.429,13.596,4.688,5.956),but the birth weights(1.71±0.23kg vs 1.83±0.26 kg)was lower(t=2.122),and the differences were statistically significant(all P＜0.05),respectively.Compared with the survival group,serum CCL11 levels(2.14±1.23 pg/ml vs 1.20±0.44 pg/ml)and IRF5 levels(8.63±3.84 pg/ml vs 4.38±1.67 pg/ml)were higher in children with NEC in the death group,and the differences were significant(t=6.052,8.178,all P＜0.001).Multifactorial logistic regression analysis showed that Bell staging for stage Ⅱ to Ⅲ(OR=1.725,95％CI=1.186～2.508),formula feeding(OR=1.429,95％CI=1.018～2.006),respiratory failure(OR=1.652,95％CI:1.121～2.435),CCL11(OR=1.641,95％CI=1.056～2.551)and IRF5(OR=1.646,95％CI=1.082～2.504)were risk factors for death in children with NEC(all P＜0.05).The AUCs of predicting the prognosis and death of NEC children with serum CCL11 and IRF5 levels alone and combination were 0.828,0.784 and 0.928,respectively,and the combination of the two was better than their respective independent predictions(Z=2.028,2.235,P=0.043,0.025).Conclusion The serum levels of CCL11 and IRF5 in NEC children are greatly elevated,and the increases in their serum levels are risk factors for death in NEC patients.Combined detection of serum CCL11 and IRF5 levels can have high diagnostic and prognostic value for neonatal NEC.