Objective To study the chemical constituents from the fungal fruiting bodies of Ganoderma sinense.Methods Individual constituents,isolated and repeatedly purified on silica gel and Sephadex LH-20 column chromatography,were identified by physicochemical constants and structurally elucidated by spectral methods.Results Four compounds were isolated and identified as: sinensoic acid(1),cerebroside D(2),poke-weed cerebroside(3),hemisceramide(4).Conclusion Compound 1 is a new compound,others are isolated from the fungus of Ganoderma for the first time.

Objective To isolate and identify the components from the stem barks of Morus yunnanensis.Methods The compounds were isolated and purified by silic gel column,Sephadex LH-20,and RP-18 chromatography.Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.Results Eleven compounds were isolated and identified as:mulberroside C(1),oxyresveratrol(2),2',4',7-trihydroxy-(2S)-flavone(3),norartocarpetin(4),moracin P(5),betulinic acid(6),sitosteryl 3?-glucoside 6'-O-palmitate(7),lupeol(8),betulinic acid(9),?-daucosterol(10),and ?-sitosterol(11).Conclusion All the compounds are isolated from the plant for the first time,and compound 9 shows cytotoxic activities.

The investigation on the leaves of Morus alba L. was carried out to find the relationship of the constituents and the pharmacological activities. The isolation and purification were performed by various chromatographies such as silica gel, Sephadex LH-20, RP-C18 column chromatography and so on. Further detailed investigation on the fraction of the ethanol extract of leaves of Morus alba yielded four Diels-Alder type adducts mulberrofuran F1 (1), mulberrofuran F (2), chalcomoracin (3), kuwanon J (4), together with two chalcones morachalcone A (5), isobavachalcone (6), and three flavones norartocarpetin (7), kuwanon C (8), 6-geranylapigenin (9). Their structures were elucidated by the spectral analysis such as NMR, MS etc. Compounds 1, 6 were isolated from this plant for the first time, compounds 4-5, 7-9 were isolated from the leaves of Morus alba L. for the first time, among which 1 was a new compound. Compounds 1-5 were evaluated for the cytotoxicity against A549, Be17402, BGC823, HCT-8 and A2780 cell lines in vitro by MTT method, but only compounds 1-3 showed cytotoxicity against several human cancer cell lines.

Objective To study the effect of nerve growth factor on airway inflammation and Th1/Th2 cells immune imbalance.Methods(1)32 SD rats were divided into 4 groups randomly:asthma,control,NGF,anti-NGF-antibody groups.The asthmatic model were established by inhalation and injection of ovalbumin.(2)We investigated the expression of NGF mRNA in the lungs in asthma and control groups by reverse transcription-polymerase chain reaction(RT-PCR).(3)In the lungs of the four groups,the expression of interferon-?(IFN-?,one of the Th1 cytokines),interleukin-4(IL-4,one of the Th2 cytokines)mRNA was detected by RT-PCR too.Results(1)As compared with the control group,NGF mRNA was significantly enhanced in the asthma group[(90.4?7.6)% vs(51.8?12.3)%,P

Objective To study the chemical constituents from the roots of Dryopteris championii.Methods The compounds were isolated by column chromatography with Sephadex LH-20 and silica gel and preparative thin layer chromatography.The structures of the compounds were identified by physico-chemical properties and spectral evidence.Results Ten compounds were identified as aspidin-BB (Ⅰ), aspidin-AB (Ⅱ), aemulin-BB (Ⅲ), methylene-bis-desaspidinol (Ⅳ), pseudo-aspidinol B (Ⅴ), methyl-phlor-butyrophenon (Ⅵ), desaspidinol (Ⅶ), hop-22(29)-ene (Ⅷ), ?-sitosterol (Ⅸ), and (E)-3-nonacosene-2-ketone (Ⅹ).Conclusion Compound Ⅰ-Ⅳ, Ⅵ and Ⅶ are isolated from this plant for the first time.Antitumor effects of aspidin-BB are investigated.

Objective To study the Diels-Alder type adducts in the stem bark of Morus mongolica.Methods The compounds were isolated and purified by silic gel column chromatography,RP18 and Sephadex LH-20.The structures were identified by various spectral evidence.Results Six Diels-Alder type adducts were obtained and they were albanin F(Ⅰ),kuwanon L(Ⅱ),dimoracin(Ⅲ),kuwanon J(Ⅳ),mulberrofuran J(Ⅴ),mulberrofuran Q(Ⅵ).Conclusion Compounds Ⅱ,Ⅲ,and Ⅴ are isolated from the plant for the first time and compounds ⅣⅥ show the anti-oxidation activities.

BACKGROUND: Hematogenesis of a body mainly depends on the proliferation of hematopoietic progenitor cell (HPC). Hematopoietic functional impairment will occur when hematopoietic cells are injured by radioactive ray or chemical drug. The proliferation of HPC is the key link of promoting hematogenesis.OBJECTIVE: To study the effects of nine monomers extracted from Spatholobus suberectus Dunn (SSD) on proliferation of HPC in marrow-depressed mice. DESIGN: Randomized controlled trial.SETTING: Department of Pharmacy, General Hospital of Chinese PLA.MATERIALS: This experiment was conducted in the Department of Clinical Pharmacology, General Hospital of Chinese PLA from November 2002 to February 2003. Totally 348 healthy Kunming mice, weighing 22-25g, clean grade, of irrespective gender, were selected in this study (certification: SCXK-2001-001). The animal experiment was approved by the local ethics committee. SSD was provided by Dispensary of Traditional Chinese Medicine, General Hospital of Chinese PLA; monomers (gallocatechin, formononetin, catechin, pyromucic acid, syringic acid, Demethylvestitol, 1,3,5-benzenetriol, ononin, and epicatechin) were extracted from SSD acetoacetate; TGL-16 centrifuger was made in Shanghai 6th Medical Equipment Factory; CO2 incubator was made in SANYO Company, Japan; MK inverted microscope was provided by OLYMPUS Company, Japan.METHODS: Experimental grouping: Mice were randomly divided into 29 groups, including normal group; control group; gallocatechin high-, medium-, low-dose groups; formononetin high-, medium-, low-dose groups; catechin high-, medium-, low-dose groups; pyromucic acid high-, medium-, low-dose groups; syringic acid high-, medium-, low-dose groups; Demethylvestitol high-, medium-, low-dose groups; 1,3,5-benzenetriol high-, medium-, low-dose groups; ononin high-, medium-, low-dose groups; epicatechin high-, medium-, low-dose groups with 12 mice in each group. Experimental intervention: All the mice except the mice in normal group had been given total body sublethal dose of irradiation by 60Co γ-ray (215.3 rontgen/min, 4 Gy dose rate, irradiation time of 107.5 seconds). Normal saline was injected intraperitoneally into 8 mice in normal group and control group at the third day after inadiation. Stored solution 2,0.4,0.08g/L of each monomer was intraperitoneally injected into the mice in each monomer high-, medium-, low-dose groups, respectively, at the third day after irradiation. Experimental evaluation: Thirty minutes after administration, blood of 8 mice in normal, control group and 12 mice in other groups was collected and normal, control and each dose monomer-containing serums were obtained after centrifugation for 15 minutes, filtering through 0.45μm filter membrane. Then 4 mice in normal and control group were killed to study the effects of nine monomers on proliferation of HPC in marrow-depressed mice by counting erythrocyte colony-forming unit (CFU-E), burst-forming uniterythroid (BFU-E), granulocyte-macrophage colony-forming unit (CFU-GM), and megakaryocyte colony-forming unit (CFU-Meg).MAIN OUTCOME MEASURES: CFU-GM, CFU-E, BFU-E, and CFU-Meg in each group. RESULTS: Totally 348 mice were included in the final analysis. CFU-E: The quantity of CFU-E in high-dose of catechin, gallocatechin, syningic acid, and epicatechin groups was significantly higher than that in the control group (P<0.05-0.01) while the quantity of CFU-E in medium-and low-dose of catechin and medium-dose of gallocatechin was also significantly higher than that in the control group (P<0.05). CFU-GM: Except pyromucic acid and ononin groups, the amount of CFU-GM in other groups was significantly higher than that in the control group (P<0.01). BFU-E: Compared with control group, the amount of BFU-E remarkably increased under the effect of each dose of catechin, gallocatechin, syringic acid and high-, medium-dose of epicatechin (P<0.05). CFU-Meg: The amount of CFU-Meg in high-, low-dose syringic acid groups, low-dose gallocatechin groups and each dose group of catechin and epicatechin was significantly higher than that in the control group (P<0.05). Amount of all colonies in the control group was significantly lower than that in the normal group (P<0.01). CONCLUSION: Nine monomers extracted from SSD can promote the proliferation of HPC in bone marrow depressed mice. In particular, the activity of catechin to stimulate proliferation is the strongest.

The investigation on the chemical constituents of Anabasis aphylla L. was carried out by using various chromatographies, such as silica gel, Sephadex LH-20 and RP-C18 column chromatography. Further detailed investigation on the fraction of the ethanol extract of Anabasis aphylla L. yielded one new compound p-acetyl-phenol 1-O-beta-D-xylopyranosyl-(1-->2)-beta-D-glucopyranoside (1), together with five known compounds: piceine (2), isorhamnetin (3), quercetin (4), rutin (5) and isorhamnetin-3-rutinoside (6). Their structures were elucidated by spectral analysis such as NMR and MS. Among these compounds, compounds 2-6 were isolated from this plant for the first time.