1.Effects of different fluid resuscitation on renal function and glycocalyx in septic shock rats
Yusi HUA ; Min HE ; Ruoran WANG ; Xiaofeng OU
Chinese Journal of Internal Medicine 2021;60(6):552-555
To investigate the effects of different fluid resuscitation on renal function and glycocalyx in septic shock rats. The septic shock SD rats (induced by lipopolysaccharide) were randomly divided into control group, normal saline resuscitation group, Lactate Ringer′s solution resuscitation group and succinyl gelatin resuscitation group ( n=6 each). The mean arterial pressure, heart rate, serum creatinine (Scr), urea nitrogen (BUN), renal blood flow, renal tissue pathology, serum interleukin-6, tumor necrosis factor α and glycocalyx were measured at baseline, the development of septic shock, 0 hour and 8 hours after resuscitation. When shock was established, the renal blood flow significantly decreased ( P<0.05) and recovered to normal at 0 hour and 8 hours after resuscitation. Compared with normal saline group and succinyl gelatin group, the levels of Scr, BUN and glycocalyx components (heparan sulfate, syndecan-1) were significant lower in Lactate Ringer′s solution group ( P<0.05). The pathology of kidney tissue suggested that the microscopic ischemic damage with Lactate Ringer′s solution were minor than the other two groups. Compared with normal saline and succinyl gelatin, Lactate Ringer′s solution can reduce the damage of renal function and glycocalyx in septic shock rats.
2.Clinical effect of neoadjuvant chemotherapy combined tumor cells to destroy the loss in treatment of patients with advanced ovarian cancer
Enling LIU ; Yuxiu ZHOU ; Ruoran MI ; Dehua WANG ; Liqun WANG ; Yanmei ZHANG
Clinical Medicine of China 2015;31(11):1041-1043
Objective To discuss the clinical effect of neoadjuvant chemotherapy combined tumor cells to destroy the loss in treatment of patients with advanced ovarian cancer.Methods One hundred and forty-four patients with advanced ovarian cancer were divided randomly into the control group(n=72) and research group (n=72).The patients of control group were given conventional chemotherapy(ovarian tumor remove first and then neoadjuvant chemotherapy) and the patients of research group were given neoadjuvant chemotherapy (neoadjuvant chemotherapy first and then ovarian tumor remove).The operation time, intraoperative blood loss, hospital stay, ideal reduction rate, clinical efficacy and postoperative complications between the two groups were compared.Results The operation time, intraoperative blood loss, hospital stay of the research group were obviously lower than that of the control group((124.6±21.3) min vs.(186.4±32.6) min, (382.5±62.3) ml vs.(618.5± 86.4) ml, (8.9± 1.3) d vs.(12.2± 3.4) d;t =5.623,9.646,5.257), while the ideal reduction rate of the research group were obviously higher than that of the control group(70.8% vs.47.2%, x2 =8.735), the differences were statistically significant(P<0.05).The clinical efficacy(87.5% vs.52.8%, x2 =6.748) of the research group were obviously higher than that of the control group, while the postoperative incision infection (9.7% vs.19.4%, x2 =4.452) and fever(4.2% vs.15.3%,x2 =5.536) were obviously lower than that of the control group, the differences were statistically significant (P<0.05).Conclusion The treatment of neoadjuvant chemotherapy can obviously increase the the clinical effect of treatment of patients with advanced ovarian cancer and decrease the postoperative complications, it is worth popularization and application.
3.Effect of VEGF-C Gene Transfection on the Expression of VEGF-C in Human Cervical Carcinoma HeLa Cells and the Molecular Meehnisms of Its Anti-apoptosis Effect
Xing CHEN ; Meifen WANG ; Zhaoyang WU ; Hongping REN ; Lingzhi ZHENG ; Shumin ZHENG ; Dongsheng XIONG ; Chunzheng YANG ; Ruoran MI
Chinese Journal of Clinical Oncology 2009;36(21):1248-1252
Objective: TO explore the effect of VEGF-C gene transfection on the expression of VEGF-C in human cervical carcinoma HeLa cells and the mechanisms of its anti-apoptosis effect. Methods: The con-structed pcDNA3.1(+)NEGF-C vector was transformed into human cervical cancer HeLa cells and was select-ed by G418. The changes in the expression level of VEGF-C mRNA and protein were determined by semi-quantitive RT-PCR and ELISA. HeLa cells with overexpression of VEGF-C were named as HeLa/S1. The expression level of NF-KB and bcl-2 mRNA was determined by RT-PCR in transfected cells. Results: After transfection by liposome, the VEGF-C mRNA level and the expression of VEGF-C protein in transfected cells were higher than those in the control groups. HeLa/S1 cell line was successfully established. In HeLa/S1 cells, the expression of NF-κB (2.06±0.09 vs 1.35±0.02 vs 1.38±0.02 P<0.05) and bcl-2 gene mRNA (2.02± 0.67 vs 0.41±0.06 vs 0.37±0.06, P<0.05) level were higher than those in the control groups. Conclusion: VEGF-C gene transfection by liposome can increase the expression of VEGF-C in human cervical cancer HeLa cells. NF-κB is stimulated and induces the overexpression of bcl-2 gene in HeLa/S1 cells.
4. Study on the status of low vision among students of Beijing in 2017
Ruoran LYU ; Yuzhen SONG ; Jiali DUAN ; Ying SUN ; Wei GAO ; Lingling WANG ; Dandan ZHANG
Chinese Journal of Epidemiology 2019;40(4):457-460
Objective:
The purpose of this study was to describe the status quo and related factors on low vision (LV) among students of Beijing.
Methods:
All the students in Beijing were included as the subjects of interest, based on the reference of the Chinese National Survey on Students’ Constitution and Health.
Results:
The overall rate of LV among students in Beijing was 58.6