Objective:To identify the potential pathogenic genes for perioperative neurocognitive disorder (PND) in the patients with digestive system tumors.Methods:The gene expression data of esophageal cancer, gastric cancer, colon cancer, rectal cancer and liver cancer in The Cancer Genome Atlas database were analyzed by bioinformatics analysis method, and the differentially expressed genes in tumor tissues in above-mentioned disease samples were identified compared with para-carcinoma tissues.Secretory proteome differential genes with the same expression trend in digestive system tumors were obtained by comparing with human secretory proteome genes.The correlation between secretomics and PND was determined by comparing with the GeneCards database.Hub genes were identified through PPI network construction and calculation, and the functions and signaling pathways of the above-mentioned differential genes were identified through GO and KEGG enrichment analysis.Results:Compared with para-carcinoma tissues, the expression of 2 640 genes was significantly up-regulated and the expression of 1 423 genes was down-regulated in esophageal cancer tissues; the expression of 3 748 genes was up-regulated and the expression of 908 genes was down-regulated in gastric cancer samples; the expression of 2 684 genes was up-regulated and the expression of 2 678 genes was down-regulated in colon cancer samples; the expression of 2 876 genes was up-regulated and the expression of 2 945 genes was down-regulated in rectal cancer samples; the expression of 1 484 genes was up-regulated and the expression of 723 genes was down-regulated in hepatocellular carcinoma samples.Among them, the expression of the encoding genes of 53 secreted proteins was uniformly up-regulated and the expression of the encoding genes of 20 secreted proteins was uniformly down-regulated in the above tumors.Twenty up-regulated genes and 3 down-regulated genes were associated with PND.PPI network analysis showed that MMP9 was the hub gene.The results of GO and KEGG analysis suggested that differentially expressed genes were mainly related to receptor-ligand activity, cytokine activity and chemokine activity, and were mainly enriched in signaling pathways related to cell cycle and cellular senescence.Conclusions:About 23 differentially expressed genes in digestive system tumors are potentially related to PND, of which MMP9 and other genes may be the hub genes, mainly acting on receptor-ligand binding, regulation of cytokine and chemokine activity, cell cycle, cellular senescence and other related signaling pathways.

Objective:To evaluate the role of acetaldehyde dehydrogenase 2 (ALDH2) in hippocampus in memory decline after myocardial ischemia-reperfusion (I/R) in rats.Methods:Twenty-four healthy male Sprague-Dawley rats, aged 2-3 months, weighing 220-280 g, were divided into 3 groups ( n=8 each) using a random number table method: sham operation group (group S), myocardial I/R group (group I/R) and ALDH2 agonist ALDA-1 group (group ALDA-1). Myocardial I/R was induced by 30 min occlusion of left anterior descending branch of coronary artery followed by 120 min reperfusion in anesthetized animals.ALDA-1 10 mg/kg was intraperitoneally injected at 5 min before ischemia in group ALDA-1.The positioning navigation training in Morris water maze test was started from 6 days before developing the model.The spatial exploration in Morris water maze test was performed at 24 h after developing the model.The rats were sacrificed after the end of behavioral experiment, and the hippocampus was extracted for microscopic examination of the pathological changes (by hematoxylin and eosin staining) and for determination of the apoptosis index (AI) (by TUNEL staining), activity of ALDH2 (by colorimetry), contents of 4-hydroxynonenal (4-HNE) and malondialdehyde (MDA) (by enzyme-linked immunosorbent assay), and expression of ALDH2 and 4-HNE (by Western blot). Results:Compared with group S, the number of crossing the original platform was significantly decreased, the time spent in the target quadrant was shortened, the activity of ALDH2 in the hippocampus was decreased, the expression of 4-HNE was up-regulated, and the contents of 4-HNE and MDA and AI were increased in group I/R ( P<0.05). Compared with group I/R, the number of crossing the original platform was significantly increased, the time spent in the target quadrant was prolonged, the ALDH2 activity was increased, the expression of 4-HNE was down-regulated, and the contents of 4-HNE and MDA and AI were decreased in group ALDA-1 ( P<0.05). There was no significant difference in ALDH2 expression in hippocampus among the three groups ( P>0.05). Conclusions:The mechanism of memory decline developed after myocardial I/R may be related to the decrease in ALDH2 activity and promotion of accumulation of aldehydes in the hippocampus of rats.

Objective:This paper briefly reviewed the literature related to skin flaps published in Chinese Journal of Plastic Surgery ( CJPS) and Plastic and Reconstructive Surgery ( PRS) in recent five years (from January 2016 to December 2020) to compare the similarities and differences between these two journals and to direct future research. Methods:In May of 2021, literature with "flap" as the keyword in the titles and abstracts published in CJPS and PRS in recent five years were searched. Related literature published by CJPS were searched by Wanfang Data and CNKI database, and that of PRS were searched by PubMed and Scopus database. After removing the repetitive literature, the titles and abstracts were read to exclude the non-flap studies and non-original articles. By reading the full text and using bibliometrics, the total number of papers published, the number of papers on flap research, the level of evidence-based medicine evidence, the nationality and organization distribution of the authors, the type of flaps, the application of flaps, and new technologies were comprehensively analyzed. Results:The total number of papers published by CJPS in recent five years was 1 116, and 244 were included in this study. The total number of articles published in PRS in the same period was 4 562, and 268 were included in this study. Most of the articles published in PRS are from American authors. The number of articles published by Chinese authors is in the second place. In the past five years, authors from the mainland of China published 21 papers in PRS. Most of the articles published by CJPS are about the pedicle flap, while PRS is about the free flap. CJPS published more articles about the traditional flaps than perforator flaps, and PRS did the opposite. CJPS published articles mainly on the local flap, anterolateral thigh flap, and peroneal artery perforator flap, while PRS focused on the inferior epigastric artery perforator fibula bone or osteocutaneous flap, and anterolateral thigh flap. The indication of flap surgery reported by CJPS is the reconstruction of various defects, while the flaps reported by PRS are mainly used in breast reconstruction and other fields. In addition, computer-aided imaging, indocyanine green angiography, propeller flap, multilobed flap, and other new technologies and concepts have been widely reported in the literature related to skin flaps published in CPJS and PRS. Conclusions:In the recent five years, the flap research in China has been at the leading international level and has certain competitiveness. However, the study in China is limited to reporting clinical experience, and the level of evidence-based medicine is relatively low, so there is still a certain gap with the international frontier research.

Objective:This paper briefly reviewed the literature related to skin flaps published in Chinese Journal of Plastic Surgery ( CJPS) and Plastic and Reconstructive Surgery ( PRS) in recent five years (from January 2016 to December 2020) to compare the similarities and differences between these two journals and to direct future research. Methods:In May of 2021, literature with "flap" as the keyword in the titles and abstracts published in CJPS and PRS in recent five years were searched. Related literature published by CJPS were searched by Wanfang Data and CNKI database, and that of PRS were searched by PubMed and Scopus database. After removing the repetitive literature, the titles and abstracts were read to exclude the non-flap studies and non-original articles. By reading the full text and using bibliometrics, the total number of papers published, the number of papers on flap research, the level of evidence-based medicine evidence, the nationality and organization distribution of the authors, the type of flaps, the application of flaps, and new technologies were comprehensively analyzed. Results:The total number of papers published by CJPS in recent five years was 1 116, and 244 were included in this study. The total number of articles published in PRS in the same period was 4 562, and 268 were included in this study. Most of the articles published in PRS are from American authors. The number of articles published by Chinese authors is in the second place. In the past five years, authors from the mainland of China published 21 papers in PRS. Most of the articles published by CJPS are about the pedicle flap, while PRS is about the free flap. CJPS published more articles about the traditional flaps than perforator flaps, and PRS did the opposite. CJPS published articles mainly on the local flap, anterolateral thigh flap, and peroneal artery perforator flap, while PRS focused on the inferior epigastric artery perforator fibula bone or osteocutaneous flap, and anterolateral thigh flap. The indication of flap surgery reported by CJPS is the reconstruction of various defects, while the flaps reported by PRS are mainly used in breast reconstruction and other fields. In addition, computer-aided imaging, indocyanine green angiography, propeller flap, multilobed flap, and other new technologies and concepts have been widely reported in the literature related to skin flaps published in CPJS and PRS. Conclusions:In the recent five years, the flap research in China has been at the leading international level and has certain competitiveness. However, the study in China is limited to reporting clinical experience, and the level of evidence-based medicine is relatively low, so there is still a certain gap with the international frontier research.