Objective:To investigate the diagnostic value of endoscopic ultrasonography (EUS) in pancreatic duct stones.Methods:The clinicopathologic data of 204 patients undergoing EUS for symptoms such as abdominal pain, abdominal distension and jaundice suspected of pancreatic duct stones, who were admitted to the General Hospital of Western Theater Command from January 2019 to April 2023 were retrospectively analyzed. Of 159 patients were finally enrolled, including 47 females and 112 males, aged (51.8±13.9) years. Surgery or endoscopic retrograde cholangiopancreatography (ERCP) is considered the " gold standard" for the diagnosis of pancreatic duct stones. Of 38 patients (23.9%) had abdominal ultrasound, 143 (89.9%) had CT scan and 93 (58.5%) had magnetic resonance cholangiopancreatography (MRCP) at the same time. The diagnostic accuracy of imaging examinations in pancreatic duct stones was compared.Results:In 159 patients, 61 (38.4%) were diagnosed of pancreatic duct stones. In the 159 patients, 61 (38.4%) were diagnosed of pancreatic duct stones by EUS. The diagnostic sensitivity, specificity, positive predictive value, negative predictive value, Youden index and accuracy of EUS for pancreatic duct stones were 98.4%, 99.0%, 98.4%, 99.0%, 97.3% and 98.7%, respectively. The accuracy of EUS in diagnosing pancreatic duct stones was higher than that of percutaneous ultrasound, CT and MRCP (χ 2=7.71, 13.76, and 5.70, P=0.012, <0.001, 0.033). The diagnostic accuracy of EUS is comparable with operation and ERCP (Kappa=0.854, P<0.001). Conclusion:EUS could be a superior imaging approach to diagnose the pancreatic duct stone.

Objective: To confirm the laboratory diagnosis of dengue bordline cases reported in Henan Province and trace its origin from molecular level in 2017. Methods: The study samples were blood samples (3-5 ml), which came from 8 suspected cases of dengue fever reported in the 2017 direct reporting system of Henan provincial infectious disease monitoring network. Meanwhile, case investigation was conducted according to National dengue fever surveillance programme. Serum were separated from blood samples and tested for Dengue NS1 antigen, IgM & IgG antibodies, and dengue RNA. According to dengue diagnosis criteria, confirmed cases were identified by testing results. Samples carried dengue RNA performed for real-time PCR genotyping and amplification of E gene. Then, the amplicons were sequenced and homological and phylogenetic analyses were constructed. Results: 8 serum samples of suspected dengue cases were collected in Henan Province, 2017. Six of them were diagnosed as dengue confirmed cases. All the dengue confirmed cases belonged to outside imported cases, 5 of them were positive by dengue RNA testing. Genotyping results showed there were 1 DENV1 case, 2 DENV2 cases and 2 DENV3 cases. A DENV2 case and a DENV3 case of this study were traced its origin successfully. The sequence of Pakistan imported DENV2 case belongs to cosmopolitan genotype, which was the most consistent with Pakistan's DENV2 KJ010186 in 2013 (identity 99.0%). The sequence of Malaysia imported DENV3 case belongs to genotype I, which was the most consistent with Singapore's DENV3 KX224276 in 2014(identity 99.0%). Conclusion The laboratory diagnosis and molecular traceability of dengue cases in Henan Province in 2017 confirmed that all cases were imported and did not cause local epidemics.

Coronary atherosclerotic heart disease is a serious threat to human life and health. In recent years, the main treatment for it is to implant the intravascular stent into the lesion to support blood vessels and reconstruct blood supply. However, a large number of experimental results showed that mechanical injury and anti-proliferative drugs caused great damage after stent implantation, and increased in-stent restenosis and late thrombosis risk. Thus, maintaining the integrity and normal function of the endothelium can significantly reduce the rate of thrombosis and restenosis. Stem cell mobilization, homing, differentiation and proliferation are the main mechanisms of endothelial repair after vascular stent implantation. Vascular factor and mechanical microenvironmental changes in implanted sites have a certain effect on re-endothelialization. In this paper, the process of injury caused by stent implantation, the repair mechanism after injury and its influencing factors are expounded in detail. And repairing strategies are analyzed and summarized. This review provides a reference for overcoming the in-stent restenosis, endothelialization delay and late thrombosis during the interventional treatment, as well as for designing drug-eluting and biodegradation stents.

Soluble epoxide hydrolase (sEH) is related to arachidonic acid cascade and is over-expressed in a variety of diseases, making sEH an attractive target for the treatment of pain as well as inflammatory-related diseases. A new series of memantyl urea derivatives as potent sEH inhibitors was obtained using our previous reported compound 4 as lead compound. A preferential modification of piperidinyl to 3-carbamoyl piperidinyl was identified for this series via structure-based rational drug design. Compound A20 exhibited moderate percentage plasma protein binding (88.6%) and better metabolic stability in vitro. After oral administration, the bioavailability of A20 was 28.6%. Acute toxicity test showed that A20 was well tolerated and there was no adverse event encountered at dose of 6.0 g/kg. Inhibitor A20 also displayed robust analgesic effect in vivo and dose-dependently attenuated neuropathic pain in rat model induced by spared nerve injury, which was better than gabapentin and sEH inhibitor (±)-EC-5026. In one word, the oral administration of A20 significantly alleviated pain and improved the health status of the rats, demonstrating that A20 was a promising candidate to be further evaluated for the treatment of neuropathic pain.