Objective To investigate the relationship between Wnt5a gene and E-cadherin or vimentin gene in breast cancer cell line MCF-7. Methods RT-PCR was used to detect the mRNA expression of Wnt5a, E-cadherin and vimentin in breast cancer MCF-7 cells and the normal human mammary epithelial cell line MCF-10A, respectively, and their correlation was analyzed. Results The mRNA expression levels of Wnt5a and E-cadherin in cell line MCF-7 were significantly lower than those in cell line MCF-10A [(16.93± 2.97)%vs. (27.47±2.76) %, (12.97±1.35) % vs. (20.43±2.60) %, both P<0.05]. The mRNA expression level of vimentin in cell line MCF-7 was significantly higher than that in cell line MCF-10A [(16.53±0.85)%(6.33± 2.08) %, P<0.05 ]. In cell line MCF-7, the expression of Wnt5a was positively related to E-cadherin (г=0.997, P<0.05), but it was negatively related to vimentin (г=-0.998, P<0.05). Conclusions The expression of Wnt5a in human breast cancer cell line MCF-7 is significantly lower than that in cell line MCF-10A, which indicates that Wnt5a is a cancer suppressor gene in breast cancer. The expression of Wnt5a in cell line MCF-7 is positively related with E-cadherin, and it is negatively related with vimentin. Wnt5a may cause invasion and metastasis of breast cancer cell through the breast epithelial mesenchymal transitions.

Egg freezing and transfer technology play an important role in addressing the issues of aging and low birthrate. However， current laws adopt a “medical model” that strictly limits this technology to medical purposes.The legal system should shift from a “medical model” to a “social model，” granting women the right to autonomously choose to freeze their eggs for non-medical indications. In conjunction with the implementation of the “social model”， it is necessary to reconstruct the principle of public welfare based on the concept of solidarity， ensuring the accessibility and fairness of technology. Meanwhile， the principle of the best interests of future generations should be prioritized to protect their well-being， and systematic supervision should be improved through the risk-monitoring model. In terms of specific rules， a pre-consultation system should be constructed to guarantee that women are fully informed and have complete self-determination in social egg freezing； the right to know the genetic origins of offspring should be confirmed to ensure that children have the right to access their genetic information； as well as an independent reproductive medicine ethics committee should be established to configure special ethical review rules suitable for egg freezing and transfer， to avoid the risk of technological abuse.

Mantle cell lymphoma (MCL) is an unique B-cell non-Hodgkin lymphoma with aggressive biological behavior, high malignancy, fast progress, bad reaction to the normal treatment, short remission after the standard treatment, which is still considered as the an incurable lymphoma type. The 59th American Society of Hematology Annual Meeting covered progress of the new target exploration of MCL, the drug resistance study and the optimization of clinical trails. The paper discusses the progress of MCL treatment according to the meeting literatures.