[ ABSTRACT] AIM:To investigate the depressant effect of FK228 combined with rapamycin on the human breast cancer cell line MCF-7 and MDA-MB-435.METHODS:FK228, a new histone deacetylase inhibitor, and rapamycin, the specific inhibitor of the mammalian target of rapamycin ( mTOR) protein, were used in the study.MCF-7 cells and MDA-MB-435 cells were exposed to different concentrations of FK228 and rapamycin.The inhibitory rate of cell growth was de-termined by SRB assay.Combination index ( CI) was used to evaluate the interaction between FK228 and rapamycin.The expression of the apoptotic proteins, cycle proteins and nucleic acid proteins were detected by Western blotting.The cell cycle was analyzed by flow cytometry.RESULTS: Both FK228 and rapamycin showed growth inhibitory effects on the breast cancer cell lines in a time-and dose-dependent manner.CI of the 2 drugs was less than 1 when the inhibitory rate of the cell growth was 50%effective dose (ED50)~ED70, indicating a synergistic effect.The combination therapy of FK228 with rapamycin increased the apoptotic proteins, and induced the down-regulation of phosphorylated Akt and over-expres-sion of caspase-3 compared with a single use of the drugs.The combination therapy of FK228 with rapamycin reduced the cycle proteins, and the cell cycle was arrested in G2/M.The levels of phosphorylated H2AX and acetylated H3 were ob-viously increased after combination therapy.CONCLUSION:The combination therapy of FK228 with rapamycin inhibits the cell proliferation and increases apoptosis with a synergistic effect, which may become a new trend for treating endometri-al cancer.

Objective: To evaluate the effect of ICU mechanical ventilation patients with cluster nursing and conventional nursing, and provide the basis for the clinical work. Methods: Randomized controlled trials (RCTs) related to the ICU mechanical ventilation patients with cluster nursing and conventional nursing were collected through the databases such as CBM、CNKI, Wanfang Data, VIP Data. Meta-analyses were conducted for the included studies by the RevMan 5.3 software. Results: A total of 12 qualified research literatures were included, with a total sample size of 1478 cases, the test group 742 cases, the control group 736 cases. Meta-analysis showed that cluster nursing intervention can reduce the time of ICU patients with mechanical ventilation (Z=14.00, P<0.01), reduce the time at ICU (Z=41.89, P<0.01), reduce the incidence of ventilator associated pneumonia (Z=8.33, P<0.01) and reduce the patient mortality (Z=2.79, P<0.01). Conclusions The results of this meta-analysis showed that cluster nursing measures were statistically significant in reducing mechanical ventilation complications in ICU patients compared with conventional nursing measures. However, in view of the methodological quality and limitations of this study included in the literature. Suggestions for future research to carry out large sample, high quality, multicenter randomized controlled study, make the ICU mechanical ventilation in patients with cluster nursing measures to constantly update and perfect, as well as to seek more evidence-based nursing support evidence.