Objective:To investigate the predictive effects of multiple biomarkers on heart failure in elderly female patients with gynecologic tumor after surgery .Methods :This retrospective study included 416 elderly female patients with gynecologic tumor after surgical treatment .Patients were divided into the heart failure group and the non‐heart‐failure group according to the NYHA classification of cardiac insufficiency . All patients underwent physical examination , laboratory examination ,ECG ,heart color Doppler sonography and preoperative evaluation of gynecology before operation ,and the heart function was evaluated again by heart color Doppler sonography after operation . White blood cell count (WBC ) , electrocardiogram fragmented QRS complex (fQRS) ,serum cardiac troponin I (TnI) and N‐terminal pro brain natriuretic peptide (NT‐proBNP) were selected as evaluation indexes for predicting cardiac function .Results:The appearance of fQRS in heart failure group was significantly higher than that in non‐heart‐failure group ,and the difference was statistically significant (P< 0 .05) .The heart failure group had significantly higher WBC ,TnI ,and NT‐proBNP levels than the non‐heart‐failure group ,and the differences were statistically significant (P<0 .05) .The sensitivity of the combination of multiple biomarkers in predicting postoperative heart failure in elderly female with gynecological tumor was 96 .2% ,and the specificity was 85 .4% . Conclusions :The combination of multiple biomarkers can be used to predict postoperative heart failure in elderly female with gynecologic tumor with simple operation methods and effective prediction results .

After treating with chemotherapy or immunosuppressant, malignant diseases of hematopoietic system such as leukemia, malignant lymphoma and aplastic anemia usually induced severe infection such as sepsis. Sepsis which is hard to be diagnosed causes high death rate. This study was purposed to establish an experimental sepsis mouse model so as to provide a basis for pathogenesis and intervention study. A classic caecal ligation and puncture (CLP) was used to establish experimental sepsis model. ELISA was used to detect levels of C5a, IL-6, TNFalpha, and IFN-gamma. Flow Cytometry was applied to measure apoptosis of lymphocytes in thymus and mesentery. The pathologic changes of thymus and spleen were confirmed by HE staining. The results showed that almost 70%-80% mice died at 72 hours after CLP. Only approximate 20% animal survived during finite time, mice in CLP group had significant weight lose. Meanwhile large release of different inflammatory mediators which are related with sepsis (C5a, IL-6, TNF-alpha, and IFN-gamma) was observed after CLP. Apoptosis of lymphocytes in thymus and mesentery lymphonodus was enhanced markedly after CLP. Significantly pathologic injury was also observed in thymus and spleen. It is concluded that a mouse model of experimental sepsis was successfully established by caecal ligation and puncture which can well mimic the clinical symptom of sepsis. The experimental sepsis mouse model provides an excellent tool for exploring the pathogenesis and intervention ways for sepsis accompanied with complicated malignant hematological diseases in vivo.
Animals ; Apoptosis ; Cecum ; injuries ; Complement C5a ; metabolism ; Disease Models, Animal ; Interferon-gamma ; metabolism ; Interleukin-6 ; metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Sepsis ; metabolism ; pathology ; Spleen ; pathology ; Thymus Gland ; pathology ; Tumor Necrosis Factor-alpha ; metabolism

The treatment ideas with acupuncture for knee osteoarthritis (KOA) are explored on the base of Dongyuan needling technology. Regarding the rules of acupoint selection, Zusanli (ST 36) is predominant, the back-shu points are used for the disorders related to the invasion of exogenous factors, and the front-mu points are for the cases caused by internal injury. Besides, the xing-spring points and shu-stream points are preferred. In treatment of KOA, besides the local points, the front-mu points, i.e. Zhongwan (CV 12), Tianshu (ST 25) and Guanyuan (CV 4), are selected specially to tonifying the spleen and stomach. The earth points and acupoints on the earth meridians (i.e. Yinlingquan [SP 9], Xuehai [SP 10], Liangqiu [ST 34], Dubi [ST 35], Zusanli [ST 36] and Yanglingquan [GB 34]) are optional to coordinate yin and yang, essence and qi , and regulate the qi movement of spleen and stomach. The shu-stream points of liver, spleen and kidney meridians (Taichong [LR 3], Taibai [SP 3] and Taixi [KI 3]) are chosen to promote meridian circulation and regulate zangfu functions.
Humans ; Osteoarthritis, Knee ; Acupuncture Therapy ; Meridians ; Acupuncture Points ; Spleen