1.Proliferation of renal mesangial cells induced by very low density lipoprotein is mediated by p42/44 mitogen activated protein kinase.
Guo-qing YU ; Wei-jie YUAN ; Ruo-lan CUI ; Peng FU
Chinese Medical Journal 2010;123(19):2710-2713
BACKGROUNDThe plasma concentration of very low density lipoprotein (VLDL) is negatively correlated to renal function in glomerular diseases. Effects of VLDL on renal function have been partially attributed to the proliferation of mesangial cells. This study examined the potential role of the p42/44 mitogen activated protein kinase (MAPK) in mesangial cell proliferation induced by VLDL.
METHODSMesangial cells were treated with VLDL at different concentrations or for different time. The cell cycle of the mesangial cells was analyzed by XTT assay and flow-cytometry; MAPK activity was also assayed. In some experiments, cells were treated with VLDL together with or without 0.1 µmol/L PD 98059.
RESULTSTen to 500 µg/ml VLDL stimulated the proliferation of mesangial cells cultured in vitro in a concentration-dependent manner. The effect was associated with an increase in p42/44 MAPK activity. Increased proliferation of mesangial cells by VLDL was significantly attenuated by PD98059, a specific p42/44 MAPK inhibitor.
CONCLUSIONThese results indicate that the p42/44 MAPK pathway is an important regulator of mesangial cell proliferation and of renal functions.
Animals ; Cell Cycle ; drug effects ; Cell Proliferation ; drug effects ; Cells, Cultured ; Lipoproteins, VLDL ; pharmacology ; Male ; Mesangial Cells ; cytology ; drug effects ; Mitogen-Activated Protein Kinase 1 ; metabolism ; Mitogen-Activated Protein Kinase 3 ; metabolism ; Rats ; Rats, Sprague-Dawley
2.Megastigmanes from an aqueous extract of Uncaria rhynchophylla
Le-ling SONG ; Yue WANG ; Ruo-fei LI ; Cheng-gen ZHU ; Qing-lan GUO ; Jian-gong SHI
Acta Pharmaceutica Sinica 2022;57(6):1832-1839
Five new megastigmanes (
3.Epidemiological characteristics and trend of pneumoconiosis in Wuxi, China during 2006-2012.
Yu-lan YAN ; Rong-ming MIAO ; Ruo-han SUN ; Yong-ming YAO ; Ping GUO ; Shen HU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(12):912-916
China
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epidemiology
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Humans
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Incidence
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Pneumoconiosis
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epidemiology
4.Minor triterpenoid acids from an aqueous extract of Uncaria rhynchophylla
Qing ZHANG ; Xiao-qiang LEI ; Ruo-fei LI ; Hua SUN ; Cheng-bo XU ; Cheng-gen ZHU ; Qing-lan GUO ; Jian-gong SHI
Acta Pharmaceutica Sinica 2023;57(4):992-1002
Seventeen minor triterpenoid acids (
5.Levels of amino acids in cerebral spinal fluid in children with cerebral palsy.
Hai-Bin YUAN ; Lian-Ying CHENG ; Fei YIN ; Guo-Xun ZHANG ; Jing PENG ; Ming-Xiu KANG ; You-Ming XU ; Ruo-Lan CHEN ; Li WANG
Chinese Journal of Contemporary Pediatrics 2008;10(4):475-477
OBJECTIVETo study the changes of amino acids in cerebral spinal fluid (CSF) in children with spastic or athetotic cerebral palsy (CP) by examining CSF levels of glutamic acid (Glu), gamma-aminobutyric acid (GABA) and aspartate (ASP).
METHODSCSF samples were obtained from 13 children with spastic CP, from 14 children with athetotic CP, and from 10 children without central nervous system and infectious diseases (control group). CSF levels of Glu, GABA and ASP were determined by high-performance liquid chromatography.
RESULTSCSF levels of GABA, ASP and Glu in the control group were 13.04+/-2.19, 10.21+/-0.45 and 8.41+/-2.26 micromol/L, respectively. Compared with the control group, CSF GABA levels in the spastic and the athetotic CP groups (8.02+/-2.03 and 10.01+/-2.68 micromol/L respectively) significantly decreased (P<0.01), whereas CSF levels of Glu (20.99+/-8.15 and 28.77+/-17.62 micromol/L respectively) and Asp (13.53+/-3.93 and 14.02+/-2.88 micromol/L respectively) in the spastic and the athetotic CP groups significantly increased (P<0.01). There were statistical differences in the GABA level between the spastic and the athetotic CP groups (P<0.05). In children with spastic CPCSF Glu level was positively correlated to muscle tension.
CONCLUSIONSCSF excitatory amino acid levels increased, while CSF inhibitory amino acid levels decreased in children with CP. There were differences for CSF amino acid levels in different types of CP. The changes of amino acid levels may contribute to the pathogenesis of CP.
Amino Acids ; cerebrospinal fluid ; Cerebral Palsy ; cerebrospinal fluid ; physiopathology ; Child, Preschool ; Chromatography, High Pressure Liquid ; Female ; Humans ; Male ; Muscle Tonus
6.Influences of bracket bonding on mutans streptococcus in plaque detected by real time fluorescence-quantitative polymerase chain reaction.
Hong AI ; Hong-fei LU ; Huan-you LIANG ; Jian WU ; Ruo-lan LI ; Guo-ping LIU ; Yun XI
Chinese Medical Journal 2005;118(23):2005-2010
BACKGROUNDEnamel demineralization occurs frequently during orthodontic treatment. In this study, we evaluated the changes of the density of mutans streptococcus (MS) in plaque after bracket bonding and using fluoride adhesive on maxillary incisors by real time fluorescence-quantitative polymerase chain reaction (RT-FQ PCR).
METHODSThe study was designed as a self-paired test. Brackets were bonded with fluoride adhesive on the left side, while non-fluoride adhesive on the right side for each patient. Plaque samples were taken from the surfaces around the brackets of four maxillary incisors before brackets bonding and after the bonding 4 weeks later. The amount of MS was measured by RT-FQ PCR. The data obtained were analyzed statistically using the SPSS 11.5 version and the alpha level was set at 0.05 (2-tailed).
RESULTSThe amount of MS in plaque increased significantly after bracket bonding (P < 0.01), whereas no significant differences were observed among four maxillary incisors both before and after brackets bonding (P > 0.05), and among the incisors using and not using fluoride adhesive (P > 0.05).
CONCLUSIONSThe increase of the density of MS in plaque after bracket bonding is one of the etiological factors for enamel demineralization in orthodontic patients. The result of this study did not support what we observed clinically that the incidence of enamel demineralization for lateral incisors was higher than that for central incisors. Using fluoride adhesive for bonding did not affect the amount of MS in plaque in our study. Further study is needed.
Adhesives ; Adolescent ; Dental Bonding ; Dental Plaque ; microbiology ; Female ; Fluorescence ; Fluorides ; administration & dosage ; Humans ; Male ; Orthodontic Brackets ; Polymerase Chain Reaction ; methods ; Streptococcus mutans ; genetics ; isolation & purification ; Tooth Demineralization
7.Effect of Individualized Moderate-intensity Exercise Prescription on Cardiopulmonary Function for Patients with Pulmonary Arterial Hypertension
Shi-li ZHU ; You-hong XIE ; Wei HUANG ; Xing-guo SUN ; Zhang-min WANG ; Xiao-dong WANG ; Wei DENG ; Ruo-lan WANG
Chinese Journal of Rehabilitation Theory and Practice 2020;26(4):479-486
Objective:To analyze the cardiopulmonary function of stable patients with pulmonary arterial hypertension (PAH), and to explore effects of the cardiopulmonary exercise testing (CPET)-based individualized moderate-intensity exercise prescription on cardiopulmonary functional reserve and exercise capacity in patients with PAH. Methods:From April, 2018 to July, 2019, 31 stable patients with PAH (PAH group) and 32 healthy counterparts (normal group) were enrolled. All subjects underwent CPET. PAH group was assessed with 6-Minute Walking Test (6MWT), and then was divided into exercise group (
8. Celastrol exerts anti-colorectal cancer effect via STAT3 inhibition
Ruo-Lan FAN ; Hai-Lan CHEN ; Bin LAI ; Wei XU ; Guo-Hong YAN ; Shao-Hua XU ; Zhi-Li LI ; Zhi-Min FAN ; Guo-Hong YAN
Chinese Pharmacological Bulletin 2022;38(11):1673-1680
Aim To investigate the anti-tumor effect of celastrol(CEL)on colorectal cancer and the possible targets/mechanisms. Methods The cytotoxic activities of CEL were evaluated against A549, HCT-116, HepG2 by CCK-8 method. Western blotting was used to detect the expression level of STAT3 and its upstream and downstream proteins(JAK2, Survivin, MCL-1)in HCT-116 cells before and after CEL treatment Flow cytometry was applied to assess CEL's apoptosis and cell-cycle arrest effect in HCT-116 cells. SPR detection and molecular docking analysis were performed to further assess the binding ability between CEL and STAT3 protein. Lastly, human colorectal cancer organoid culture was constructed to verify the anti-tumor effect of CEL. Results CEL showed significant cytotoxicity to A549(IC50 = 2.37±0.02 μmol·L-1), HCT-116(IC50 = 1.40±0.21 μmol·L-1)and HepG2(IC50 = 2.52±0.02 μmol·L-1). Additionally, CEL could effectively decrease the level of p-STAT3 and the downstream gene expression of STAT3(Survivin and MCL-1)in a concentration-dependent manner; however, CEL did not affect the total level of STAT3 and upstream kinases JAK2. Moreover, CEL could induce apoptosis of HCT-116 cells concentration-dependently and arrest the cell cycle. According to the SPR analysis, CEL showed a strong binding affinity with the KD value(the equilibrium dissociation constant)of 60.38 μmol·L-1. Molecular docking analysis also suggested that CEL bound to the SH2 domain of STAT3. Lastly, CEL showed much better activity than the positive drug oxaliplatin(L-OHP)on all the colorectal cancer organoids. Conclusions CEL shows a significant anti-colorectal cancer effect, potentially caused by a direct target inhibiting STAT3, inducing apoptosis, and blocking the cell cycle.
9.Two folate-derived analogues from an aqueous decoction of Uncaria rhynchophylla.
Yue WANG ; Qing-Lan GUO ; Ruo-Fei LI ; Cheng-Bo XU ; Cheng-Gen ZHU ; Jian-Gong SHI
Chinese Journal of Natural Medicines (English Ed.) 2019;17(12):928-934
Two new folate-derived analogues, named uncarophyllofolic acids A (1) and B (2), respectively, were isolated from the Uncaria rhynchophylla hook bearing stem (Gouteng in Chinese). The distinct stereochemical structures of 1 and 2 were determined by spectroscopic data analysis in combination with acidic hydrolysis and Marfey's derivatization, along with comparison of their specific rotation and Cotton effect (CE) data with those of the biogenetically related known derivatives as well as theoretical calculations of electronic circular dichroism (ECD) spectra. A plausible biosynthetic pathway of 1 and 2, associating to folate metabolism and the previously reported orychophragines A-C from Orychophragmus violaceus, is discussed.