1.Convolution-based restoration approach to the double fractures of the radius and ulna.
Xiao-Rong ZHENG ; Li-Juan ZHONG ; Qing-Rui XIAO ; Zhen-Sheng WANG ; Ruo-Fan YE ; Xiao-Bao WU
China Journal of Orthopaedics and Traumatology 2012;25(10):875-876
Adolescent
;
Adult
;
Child
;
Child, Preschool
;
Female
;
Humans
;
Male
;
Manipulation, Orthopedic
;
methods
;
Radius Fractures
;
therapy
;
Ulna Fractures
;
therapy
2.Detection of early organ dysfunction for the selection of treatment strategy on severe acute pancreatitis..
Yi-Fan LU ; Ruo-Qing LEI ; Zhi-Wei XU ; Yang DENG ; Sheng CHEN ; Hong-Chang LI ; Chun-Yu CHAI ; Tian-Quan HAN ; Yao-Qing TANG ; Sheng-Dao ZHANG
Chinese Journal of Surgery 2009;47(19):1455-1458
OBJECTIVETo investigate the severity related influencing factor and treatment strategy of severe acute pancreatitis with early organ dysfunction.
METHODSFrom July 2007 to December 2008, 167 patients with severe acute pancreatitis were treated in the Surgical Department of Ruijin Hospital. The relationships between the happening of early organ dysfunction and outcome of the patients were observed, with operative or nonoperative treatment strategy.
RESULTSAmong 167 patients, 68 patients have early organ dysfunction, in which 39 with single organ dysfunction and 29 with multiple organ dysfunction. The early organ dysfunction were involved in 47.1% in cardiovascular system, 35.3% in lung and 29.4% in kidney. Aging (P < 0.05) and higher APACHE II score (P < 0.05) predicted a poor prognosis, which were benefit from early operation.
CONCLUSIONSThe mortality of the patients with SAP is related to age, and the degree of organ dysfunction as well. In the first phase of the disease, the selection of operation depends on the trends and the degree of early organ dysfunction before infected necrosis happens, with the aid of SOFA score as a scale.
Acute Disease ; Aging ; Humans ; Multiple Organ Failure ; Pancreatitis ; diagnosis ; Prognosis
3.Design and experiment of the pedicle aiming device for the cervical spine.
Dong-sheng HUANG ; Pei-qiang SU ; Ruo-fan MA ; Chun-hai LI ; Yan PENG ; Shang-li LIU
Acta Academiae Medicinae Sinicae 2005;27(2):156-159
OBJECTIVETo probe the accuracy and safety of using an aiming device in the transpedicular fixation of cervical spine.
METHODSEight cervical specimens were obtained. We used the computed tomography (CT) to scan C3 to C7, measured the medial angle of the pedicle, and determined the location of the pedicle projecting on the articular process. Then we took the oblique X-ray film, measured the cephalic/caudal angle of the pedicle, and determined the location of the pedicle projecting on the articular process. All the specimens were equally divided into two groups. Screws of 2.8 mm x 30 mm, were used. Specimens in one group were inserted with the transpedicular screw manually, while specimens in the other one inserted with the transpedicular screw using a self-designed aiming device that can be modulate at the three dimensions according to the angles of the pedicles.
RESULTSThe first group totally had 40 screws from C3 to C7. There were 13 screws in the pedicle, 9 violated the walls of the pedicle but not involved the adjunct structure, and 18 injured the important structure such as spinal cord, verteberal artery, or nerve root. In the other group, only 4 screws violated the walls of the pedicle but not involved the adjunct structure, and the others all in the pedicles. The difference was of statistical significance (P < 0.01).
CONCLUSIONIn the cervical spine, transpedicular fixation using an aiming device can improve the accuracy and safety during operation.
Adult ; Bone Screws ; Cervical Vertebrae ; diagnostic imaging ; surgery ; Female ; Humans ; Internal Fixators ; Male ; Orthopedic Fixation Devices ; Spinal Fusion ; instrumentation ; methods ; standards ; Stereotaxic Techniques ; Therapy, Computer-Assisted ; Tomography, X-Ray Computed
4.Histogram Analysis of Diffusion Kurtosis Magnetic Resonance Imaging for Diagnosis of Hepatic Fibrosis.
Ruo Fan SHENG ; Kai Pu JIN ; Li YANG ; He Qing WANG ; Hao LIU ; Yuan JI ; Cai Xia FU ; Meng Su ZENG
Korean Journal of Radiology 2018;19(5):916-922
OBJECTIVE: To investigate the diagnostic value of diffusion kurtosis imaging (DKI) histogram analysis in hepatic fibrosis staging. MATERIALS AND METHODS: Thirty-six rats were divided into carbon tetrachloride-induced fibrosis groups (6 rats per group for 2, 4, 6, and 8 weeks) and a control group (n = 12). MRI was performed using a 3T scanner. Histograms of DKI were obtained for corrected apparent diffusion (D), kurtosis (K) and apparent diffusion coefficient (ADC). Mean, median, skewness, kurtosis and 25th and 75th percentiles were generated and compared according to the fibrosis stage and inflammatory activity. RESULTS: A total of 35 rats were included, and 12, 5, 5, 6, and 7 rats were diagnosed as F0–F4. The mean, median, 25th and 75th percentiles, kurtosis of D map, median, 25th percentile, skewness of K map, and 75th percentile of ADC map demonstrated significant correlation with fibrosis stage (r = −0.767 to 0.339, p < 0.001 to p = 0.039). The fibrosis score was the independent variable associated with histogram parameters compared with inflammatory activity grade (p < 0.001 to p = 0.041), except the median of K map (p = 0.185). Areas under the receiver operating characteristic curve of D were larger than K and ADC maps in fibrosis staging, although no significant differences existed in pairwise comparisons (p = 0.0512 to p = 0.847). CONCLUSION: Corrected apparent diffusion of DKI histogram analysis provides added value and better diagnostic performance to detect various liver fibrosis stages compared with ADC.
Animals
;
Carbon
;
Diagnosis*
;
Diffusion*
;
Fibrosis*
;
Liver
;
Liver Cirrhosis
;
Magnetic Resonance Imaging*
;
Rats
;
ROC Curve
6.Effect of interleukin-6 on the chondrocytes in the cartilage endplate of rabbits in vitro.
Wei YE ; Ruo-Fan MA ; Yue DING ; Dong-Sheng HUANG ; Wei-Jian CHEN ; Yan PENG ; Shang-Li LIU
Journal of Southern Medical University 2007;27(8):1187-1189
OBJECTIVETo evaluate the effect of interleukin-6 (IL-6) on the biological behaviors of the chondrocytes in the cartilage endplate of rabbits.
METHODSChondrocytes isolated from the cartilage endplate of New Zealand rabbits, verified for their biological characteristics by such means as toluidine blue staining for type II collagen, were treated with IL-6 at different concentrations. The proliferation of the chondrocytes was evaluated by MTT assay at different time points following the treatment, the cell cycle changes were determined by flow cytometry and the changes of aggrecan and type II collagen mRNAs detected by RT-PCR.
RESULTSAt the concentrations of 10, 50 and 100 ng/ml, IL-6 did not obviously affect the rate of chondrocyte proliferation. IL-6 at 50 ng/ml resulted in no obvious changes of the cell cycle of the chondrocytes, but significantly decreased the expression of collagen IIa mRNA.
CONCLUSIONIL-6 has no effect on the proliferation and cell cycle of the chondrocytes, but at higher concentrations, it inhibits matrix synthesis of the chondrocytes to promote intervertebral disc degeneration.
Aggrecans ; genetics ; Animals ; Cartilage ; cytology ; Cell Cycle ; drug effects ; Cell Proliferation ; drug effects ; Chondrocytes ; cytology ; drug effects ; metabolism ; Collagen Type II ; genetics ; Female ; Gene Expression Regulation ; drug effects ; Interleukin-6 ; pharmacology ; Male ; RNA, Messenger ; genetics ; metabolism ; Rabbits
7.The risk factors and preventive strategies of heterotopic ossification after artificial disc replacement in lumbar spine.
Dong-sheng HUANG ; An-jing LIANG ; Wei YE ; Pei-qiang SU ; Shang-li LIU ; Ruo-fan MA ; Bi-ling LIANG ; Guo-zhao LI
Chinese Journal of Surgery 2006;44(4):242-245
OBJECTIVETo analyze the risk factors of heterotopic (HO) ossification after total lumbar disc replacement and probe the preventive strategies for it.
METHODSThe radiographs and clinical data of 78 discs in 65 patients who received artificial lumbar disc replacement (ADR) from April 1998 to December 2003 were analyzed retrospectively by two radiologists and one orthopaedic surgeon and then postoperative HO were graded according to McAfee system. The bony formations in disc spaces, time of HO were found, and range of motion (ROM) of the operated levels were measured on radiographic films. In addition, the risk factors such as preoperative peri-annulus ossification, bony endplates injuries, and subsided or mal-position of the prostheses were also analyzed by Logistic regression analysis.
RESULTSPostoperative HO was found in 10 spaces of 9 cases. Class I of HO were occurred in 7 patients at means 2.1 years postoperatively with normal range of motion preserved. Three of them turned into class II or III with 10 degrees of mean ROM in the following 2.5 years. Another 2 (2/9) cases with preoperative peri-annulus ossification had bridging trabecular bone (class III) between the endplates and 9 degrees of ROM 2 years after surgery, then turned into class IV at 6 years with 0 degrees and 4 degrees of motion in the operated levels. As the risk factors of HO, preoperative annulus ossification (2 cases), bony endplates injuries (5 cases), mal-positioned prostheses (2 cases) and subsided prostheses (2 cases) were found simultaneity with significant positive relation to HO occurred (P < 0.05).
CONCLUSIONSFactors such as preoperative ossification of annulus, endplate injuries, prosthesis subsided and mal-position would have higher risks to have HO occurred after ADR, but ROM of most affected levels are preserved. Strict control indication and avoid all above risk factors can prevent HO occurring effectively.
Adult ; Aged ; Diskectomy ; Female ; Follow-Up Studies ; Humans ; Lumbar Vertebrae ; surgery ; Male ; Middle Aged ; Ossification, Heterotopic ; etiology ; prevention & control ; Prosthesis Implantation ; adverse effects ; Retrospective Studies ; Risk Factors
8.Epidemiological characteristics and drug resistance surveillance of Shigella in infants and young children in Henan Province from 2013 to 2017
Yu-jiao MU ; Ruo-lin WANG ; Bai-fan ZHANG ; Jia-yong ZHAO ; Meng-lei LI ; Sheng-li XIA ; Xue-yong HUANG
Chinese Journal of Disease Control & Prevention 2019;23(7):835-839
Objective To investigate the pathogenic characteristics of Shigella in infants from 2013 to 2017 in Henan Province. Methods From 2013 to 2017, 606 Shigella strains were isolated from 5 149 children with diarrhea under 5 years old in Henan Province. Serotyping, drug sensitivity test and Polymerase Chain Reaction detection of virulence gene methods were used to detect the pathogen of Shigella. Results The detection rate of Shigella in children with diarrhea was 11.77%, and the highest detection rate was in the 1-2 age group(24.08%). 606 Shigella strains were divided into two groups and 11 serotypes. Shigella flexneri accounted for 73.43%, and Shigella sonnei accounted for 26.57%. Resistance of 176 Shigella strains to ampicillin and naphthidine was serious (resistance rate > 90%), and the resistance rates to chloramphenicol, ciprofloxacin, norfloxacin and compound sulfamethoxamine were higher than 65%, and the sensitivity of imipenem and cephalosporin were higher. There were differences in drug resistance between Shigella flexneri and Shigella sonnei. The virulence genes of infants were mainly shET-1+, shET-2+, ipaH+ and ial+, and 5 avirulent strains were detected. Conclusions The bacterial dysentery of infants in Henan Province is dominated by Shigella flexneri. There are serious resistance and multidrug resistance to common antibiotics, and the dominant genes in different serotyping strains are different.
9.Study of magnetic resonance imaging based on liver imaging reporting and data system for evaluating phosphatidylinositol proteoglycan-3 expression in hepatocellular carcinoma.
Wei SUN ; Jiang Tao ZHAO ; Shan Shan GAO ; Jing HAN ; Ruo Fan SHENG ; Mengsu ZENG
Chinese Journal of Hepatology 2022;30(8):866-872
Objective: To clarify the diagnostic value of magnetic resonance imaging based on liver imaging reporting and data system (LI-RADS) for phosphatidylinositol proteoglycan-3 (GPC3) expression in hepatocellular carcinoma (HCC). Methods: Clinical and pathological data of 95 HCC cases with positive GPC3 expression (+) and 40 HCC cases with negative GPC3 expression (-) were retrospectively analyzed, and their MRI image features based on the 2018 version of LI-RADS were compared. Multivariate logistic regression analysis was used to determine the main predictors of GPC3 expression. Receiver operating characteristic curve was used further to determine the diagnostic efficacy of combined clinical imaging model to predict GPC3 expression. Enumeration data were compared with χ2 test or Fisher's exact test. Measurement data were compared using independent samples t-test or Mann-Whitney U-test. Results: There were statistically significant differences between HCC in GPC3 (+) and GPC3(-) group at alpha-fetoprotein (AFP) levels (χ2=31.814, P<0.000 1), and MRI features: capsular enhacement (χ2=4.108, P=0.043), halo type enhancement (χ2=4.847, P=0.028), and lesion apparent dispersion coefficient (ADC) (t=2.552, P=0.011 8). Multivariate regression analysis showed that AFP>20 μg/L (OR=9.358, P<0.000 1) and ADC≤1.404×10-3 mm2/s (OR=1.003, P=0.017) were independent predictors for GPC3 expression in HCC. The combined model and the area under the curve value for the diagnosis of GPC3(+) in HCC was 0.810, and its diagnostic sensitivity and specificity were 76.8% and 77.5%, respectively. Conclusion: AFP>20 μg/L and ADC≤1.404×10-3 mm2/s may indicate the expression of GPC3 in HCC, and the combination of the two diagnostic indicators can provide a simple and effective non-invasive diagnostic method for clinical practice.
Biomarkers, Tumor
;
Carcinoma, Hepatocellular/pathology*
;
Glypicans/metabolism*
;
Humans
;
Liver Neoplasms/pathology*
;
Magnetic Resonance Imaging
;
Phosphatidylinositols
;
Retrospective Studies
;
alpha-Fetoproteins/metabolism*
10.Exploring the mechanism of anti-hereditary Parkinson's disease of baicalein based on PINK1 RNAi Drosophila model
Sheng-hui HAO ; Ruo-fan JIA ; Jiao-rui WANG ; Li GAO ; Xue-mei QIN ; Guan-hua DU ; Jian-qin ZHANG
Acta Pharmaceutica Sinica 2023;57(3):672-678
The aim of this study was to investigate the effect of baicalein on a