BACKGROUND:For a long time, the myocardium of adult mammalians is the terminal y differentiated tissue with no regeneration capacity. If damaged, myocardial cells wil be replaced by fibrous connective tissue. OBJECTIVE:To rediscover the myocardial cells and to do a review for cardiac stem cells, in order to define the existence of myocardial cells. METHODS:A computer-based online research of CNKI and PubMed databases was performed to col ect articles published between 2003 and 2014 with the key words of“cardiac stem cells, stem cells, cardiac regeneration”in Chinese and English, respectively. There were 82 articles after the initial survey, and final y 40 articles were included in result analysis. RESULTS AND CONCLUSION:Cardiac stem cells exist in the heart, and some surface markers of cardiac stem cells have been discovered. Cardiac stem cells for some diseases with myocardial cellinjury have opened up a new way. Because of the smal number of myocardial cells, how to purification, culture, identification and proliferation wil be further studied to meet the need for regenerative medicine and tissue engineering. Cardiac stem cellresearch wil open a new approach for cardiac tissue engineering.

Bilingual education refers to applying a kind of foreign language to the non-language class curriculum,and the foreign language and the professional knowledge are gained in synchronization.Using bilingual education has increased students' study difficulty in the medical course.The object of Taskbased Teaching is that the student must grasp the specialized knowledge and the foreign language.

Objective To screen differential Mycobacterium tuberculosis genes between Xinjiang clinical strains and H37Rv by suppression subtractive hybridization( SSH), and to analyze the function of these specifically pathopoiesis genes. Methods Both M. tuberculosis Xinjiang clinical strains and H37Rv as tester and driver each other, most identical genome was drived whereas some distinctive genes was re-mained and enriched by utilization SSH technique. Meanwhile through inserting differential genes to E. coli all of sequences that we have cloned were determined by BLAST in GenBank. The function of differential genes between M. tuberculosis H37Rv and Xinjiang clinical strains were analyzed. Results We cloned and analyzed six different DNA fragments that only existed in Xinjiang clinical strains. One is the fragment of a gene ceding monooxygenase, flavin-binding family identified by Glimmer2. One fragment belongs to acyl-transferase family protein. One for aminotransferase, class Ⅱ, acyl carrier protein. One fragment belongs to chromosomal replication initiator protein DNA and one for M. tuberculosis paralogous family 11-pyridoxamine 5'-phosphate oxidase-related. Meanwhile, we cloned ten DNA fragments only in H37Rv. Conclusion SSH technique can efficiently screen differential genes in M. tuberculosis in Xinjiang clinical strains. They are possible key genes that M. tuberculosis survive and fortify virulence in mal-environment as same as their ho-mogenic genes, such as enhanced adsorbability in wall-held protein, counteracted digestion by nitro-oxygen-ase, elevated composition capability in the acyhransferase, control chromosomal replication initiator protein, synthesized aminotransferase acyl cartier protein and pyridoxamine 5'-phosphate oxidase.

Objective To discuss the characteristics of ultrasonic images obtained by color Doppler per rectum after intrarectal pouring and the advantages of this method.Methods 40 cases who were diagnosed as rectal carcinoma prior to surgeries were investigated.The rectum was cleand and then was poured with normal saline before examination.Color Doppler ultrasonography per rectum was performed with intrarectal probe touching the inferior edge of the tumor.The 2-dimensional images and color Doppler images were then analyzed.Results The accurate typing of rectal tumors(detailedly,the ulcerative,intumescent,and confined types)can be obtained with this method.Within the 40 cases,24 were ulcerative,12 were intumescent and 4 were confined.Only one case with ulcerative tumor was misdiagnosed as intumescent.The total accuracy rate was 97.5%.As with rectal tumors less than 5cm,there was no significant difference between the measurement by ultrasonography and that by pathologic methods (P＞0.05).While for tumors larger than 5cm,the measurement by ultrasonography was significantly different from that by pathologic methods (P＜0.05).There was no significant correlation between the bloodstream signal of color Doppler and pathological grading(P＞0.05).The method can be applied for patients who have a narrow rectal duct or are suffering from agonies which prevent the passage of the colonoscope.For cases failing to detect the margin and surface of the tumor with routine method,or ultrasonic probe failing to pass the confined area leading to illegible 2-dimensional images,This method is competent as well.Conclusion Color Doppler ultrasonography per rectum after intrareetal pouring can be applied for the diagnosis of rectal tumors of all types.

Objective To compare the effectiveness of two kinds of defluorinating agents (bone char and modified clay) for removal of fluoride in drinking water,and to analyze their application feasibility in small towns.Methods Influencing factors on defluorinating efficiencies of the two kinds of defluorinating agents under the same conditions were studied,namely the effects of adsorption time,temperature,pH,common anions and cations,and solid-liquid ratio on defluorinating efficiencies were studied.The defluorinating efficiencies of the two kinds of defluorinating agents were validated by original groundwater in Jilin Province.The homemade bone char defluorinating agent and modified clay defluorinating agent were regenerated by NaOH and Al2(SO4)3,respectively,and their regeneration abilities were investigated.Results The adsorption data of the two kinds of defluorinating agents were fitted Langmuir model well,and the maximum adsorption capacities were 10.225 g/kg(bone char) and 0.587 g/kg(modified clay).The effect of temperature on defluorinating efficiencies was limited,with increasing temperature(10-35 ℃),the adsorption capacity of bone char and modified clay for fluoride ranged from 1.568 g/kg to 1.598 g/kg and 0.423 g/kg to 0.470 g/kg,respectively.Fluoride could be easily removed under low pH conditions,with increasing of pH(4-10),the adsorption capacity of bone char and modified clay for fluoride decreased from 1.598 g/kg to 1.523 g/kg and 0.724 g/kg to 0.320 g/kg,respectively.The defluorinating efficiencies were improved by common cations(Ca2+) in groundwater,and the adsorption capacity of bone char and modified clay for fluoride increased to 1.713 g/kg and 0.485 g/kg,respectively.Whereas,the defluorinating efficiencies were inhibited by common anions(HCO3-,SO42-) at a certain degree,and the adsorption capacities of bone char and modified clay for fluoride were decreased to 1.158 g/kg(HCO3-) and 0.237 g/kg(SO42),respectively.Defluoridation bone char could be regenerated by soaking in NaOH,and modified clay by immersion in Al2(SO4)3.The adsorption capacities of the two defluorinating agents that had been regenerated for three times still reached 94.6％ and 97.3％ of their original capacities.Conclusions Both of the homemade bone char defluorinating agent and modified clay defluorinating agent have good defluorinating efficiencies and can be regenerated for many times,which are perfectly suitable for application in small towns since they are simple and can be easily applied.The homemade bone char defluorinating agent is suitable for family drinking water and the homemade modified clay defluorinating agent would be more suitable for centralized drinking water.

The basic information and clinical application of nutritional risk scales for children with cancer were reviewed, and the strengths and weaknesses of each scale were analyzed. After systematic search and reading, the scales with more clinical applications included universal scales: Pediatric Malnutrition Assessment Screening Tool (STAMP), Nutritional Risk and Stunting Malnutrition Screening Tool (STRONG kids), Pediatric Yorkhill Malnutrition Score (PYMS), Pediatric Subjective Global Nutritional Risk Assessment (SGNA); specific scales: Nutritional Screening Tool for Childhood Cancer (SCAN), Nutritional Risk Screening for Childhood Cancer (NRS-PC). In order to effectively manage the nutritional risk of pediatric cancer patients, we should selectively use and further actively Chinese or develop specific nutritional risk measurement tools adapted to our national conditions.