Objective To explore the value of plasma brain natriuretic peptide test in the diagnosis and treatment of elderly heart failure patients with normal ejection fraction,and to provide reference for clinical diagnosis and treatment of the disease.Methods 104 patients with heart failure were selected,at the same time,30 elderly patients with normal heart function in our hospital were selected as the control group.The plasma brain natriuretic peptide and cardiac color Doppler ultrasound were examined.The plasma brain natriuretic peptide and left ventricular ejection fraction,left ventricular posterior wall thickness,left ventricular mass index,left ventricular diastolic diameter and ventricular septal thickness were compared between two groups ; at the same time,peptide and left ventricular plasma brain natriuretic ejection fraction were compared in heart failure patients with different levels of NYHA.Results The plasma level of brain natriuretic peptide,left ventricular posterior wall thickness,left ventricular mass index in the heart failure group were (401.37 ± 368.63) ng/L,(13.42 ± 1.33) mm,(130.62 ± 9.18) g/m2,which were significantly higher than those of the control group (all P ＜ 0.05).The left ventricular ejection fraction of the heart failure group was (51.36 ±4.41) ％,which was significantly lower than the control group(P ＜0.05).The left ventricular ejection fraction of patients with different NYHA grading in the heart failure group was significantly lower than that of the control group(all P ＜0.05),plasma brain natriuretic peptide levels in patients with different NYHA grading were significantly higher than those in the control group,and with the elevated levels of NYHA classification,the plasma brain natriuretic peptide level increased significantly(P ＜ 0.05).Conclusion The level of plasma brain natriuretic peptide in elderly heart failure patients with normal ejection fraction was significantly increased,and with the increase of NYHA grade,plasma brain natriuretic peptide level rised,it is important to assess diagnosis and severity of this disease.

Objective:To investigate the effect and the mechanism of Apigenin on lipopolysaccharides ( LPS )-induced inflammatory mediators production in murine macrophages. Methods:The murine macrophage cell line RAW 264. 7 cells were cultured in vitro,and were treated with different concentration of Apigenin followed by LPS administration. Expression of heme oxygenase-1 ( HO-1),cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS),phosphorylation of p38 and IκB,nuclear translocation of Nrf2 were detected by Western blot. Production of Nitrite and nitrate ( NOx) was analyzed by colorimetric technique. Secretion of prosta-glandin E2 (PGE2) was detected by ELISA. Activation of NF-κB was measured by luciferase assay. Results: Western blot indicated that apigenin could induce RAW 264. 7 cells expression of HO-1, and pretreatment of SB203580, an inhibitor of p38 significantly inhibited apigenin induced HO-1 expression. In addition,Apigenin could also decrease the content of nuclear transcription factor Nrf2 in cytoplasm and increase its level in the nucleus. Silencing of Nrf2 by specific siRNA could inhibit apigenin-induced HO-1 expression. Furthermore,apigenin administration significantly inhibited LPS-induced NOx production and PGE2 secretion, COX-2 and iNOS expression,IκB phosphorylation and NF-κB activation,and transfection of HO-1 siRNA could reverse these actions. Conclusion:Apigenin inhibits LPS-induced inflammatory response through induction of HO-1 and inhibition of NF-κB in macrophages.

Objective: To explore the effect of ISBAR communication mode on the bed shift in severe patients. Methods: The "ISBAR Nursing Delivery Card near the Bed of Severe Diseases Medicine" was formulated. 100 cases of severe patients admitted from February 2018 to March 2018 were set up as control group by experimental research methods. 100 cases of severe patients admitted from April 2018 to May 2018 were set up as experimental group and non-random control was established. The patients in the experimental group were treated with ISBAR nursing shift card for bed shift, and the control group were treated with normal oral shift. Observe and compare the incidence of nurse shift problems, the score of nurse shift assessment scale and the nurse′s knowledge of the patient′s condition "ten know" score between the two groups. Results: The incidence of nurse shift problems dropped from 39.50% (79/200) of control group to 16.50% (33/200) of experimental group, which had a significant difference (χ²=42.938, P<0.01). The score of the nurse′s succession assessment table was increased from (57.80 ± 3.61) points of control group to (73.96 ± 2.33) points of experimental group, which had a significant difference (t=-26.10, P<0.05). The nurse′s knowledge of the patient′s condition "ten know" had been increased from (72.14±4.13) points of control group to (87.75±2.54) points of experimental group, which had a significant difference (t=-32.01, P<0.05). Conclusion The use of ISBAR communication mode for serious patients in the severe medical department can effectively reduce the incidence of nurse shift problems, effectively improve nurses 'satisfaction with shift and the degree of mastery of patients′ conditions, and the effect of shift is better.