1.Research advances in SULF2 and its relationship with hepatocellular carcinoma
Shaoshan HAN ; Runkun LIU ; Lei HAN ; Qingguang LIU
Chinese Journal of Hepatobiliary Surgery 2021;27(1):74-77
Cell secreted sulfatase 2 is an endogenous sulphate esterase, which can hydrolyze the sulphate groups in extracellular matrix or on the chain of heparan sulphate on the outer surface of cell membrane, which can dissociate the growth factor bound to heparan sulphate proteoglycan, improve the local concentration of growth factor and activate the downstream signal. Sulfatase 2 is highly expressed in a variety of tumors, and the increased expression of sulfatase 2 in hepatocellular carcinoma is associated with poor prognosis. Sulfatase 2 can promote tumor progression by activating multiple signaling pathways in hepatocellular carcinoma cells. Inhibit the activity of hepatocellular carcinoma cell sulfatase 2, and then inhibit the proliferation, migration and tumorigenesis of hepatocellular carcinoma cells. In this paper, the research progress of sulfatase 2 and its role in the occurrence and development of hepatocellular carcinoma is summarized.
2.Methyl rosmarinate induces cell apoptosis in human hepatocellular carcinoma cells via inhibiting the Akt/mTOR signaling pathways
Yao XIAN ; Wei JIANG ; Runkun LIU ; Kangsheng TU ; Shijie GAO ; Jun WANG ; Lei ZHANG
Journal of Xi'an Jiaotong University(Medical Sciences) 2023;44(5):802-808
【Objective】 To investigate the cell death-inducing effect of methyl rosmarinate (MR) on human hepatoma Hep-3B and SK-Hep1 cells and their potential mechanisms. 【Methods】 The effects of MR on the viability of Hep-3B, SK-Hep1 and MIHA cells were determined by cell counting kit-8 (CCK-8) assay. The morphological changes of three kinds of cells treated with different concentrations of MR were observed by optical microscopy. EdU assay and flow cytometry were used to detect the proliferation and apoptosis of Hep-3B and SK-Hep1 cells. Transwell assay was used to study the effects of MR on the migration and invasion of Hep-3B and SK-Hep1 cells. Western blotting was used to evaluate the protein expression levels of apoptosis, EMT and Akt/mTOR signaling pathways. 【Results】 After treated with different concentrations of MR (0~200 μmol/L) for 48 h, Hep-3B and SK-Hep1 cells activities were significantly decreased in a concentration-dependent manner (P<0.01), while there was no significant effect on MIHA cell activity (P>0.05), and the IC