Objective To investigate the effects of Danshen Duofensuanyan on the blood coagulation,inflammatory and cardiac function in acute myocardial infarction(AMI) patients who did not accept emergency percutaneous coronary intervention.Methods One hundred and twenty patients,from January 2012 to November 2014 in the First Hospital of Qinhuangdao admission due to acute myocardial infarction frequent episodes of angina(Killip grade Ⅱ,grade m),were randomly divided into two groups:control group (n =60) and observation group (n =60).All patients suffered from severe angina after AMI and heart failure but without PCI.All included patients were gave antiplatelet drug,anticoagulants and Statins,etc.And the patients in observation group were additionally given 7-day Danshen Duofensuanyan injection 200 mg/d once daily intravenously.Platelet aggregation rate (PAG),fibrinogen (FIB),c-reaction protein (CRP),erythrocyte sedimentation rate (ESR),troponin Ⅰ (TNI),plasma brain natriuretic peptide (BNP) and left ventricular end diastolic dimension(LVEDD),left ventricular ejection fraction (LVEF) and cardiac output (CO) of two group were monitored before and after treatment.Results (1)Changes in coagulation,inflammatory cytokines and enzymes between the treatment group and the control group after treatment (Treatment Group:PAG (22.32 ±17.61) ％,FIB(3.58±0.74) g/L,CRP(9.34±1.82) mg/L,ESR(10.1±4.6) mm/1 h,TNI(4.51± 1.89) ng/ml);control group:PAG(31.32±21.62)％,FIB(3.84±0.62) g/L,CRP (14.32±1.79) mg/L,ESR(13.2 ± ±5.4) mm/1 h,TNI(5.32±2.31) μg/L) and before treatment(Treatment group:PAG(88.87± 18.21) ％,FIB (4.78±0.97) g/L,CRP(32.13±11.59) mg/L,ESR(28.5±6.1) mm/1 h,TNI (56.43 ± 21.87) μg/L);control group:PAG (89.53± 19.35) ％,FIB (4.66 ± 0.78) g/L,CRP (29.06± 12.47) mg/L,ESR (29.3 ± 3.2) mm/1 h,TNI (53.69± 18.76) μg/L) were significandy improved,and the differences were statistically significant (P＜0.01),and observation group had more significant improvements than the control group(P＜0.05 or P ＜0.01) after treatment.(2) All aboved indexes were improved after treatment except LVEDD.And Danshen Duofensuanyan can significantly improve PAG,FIB,CRP,ESR,TNI,BNP LVEF and CO(P＜0.05 or P ＜0.01).Conclusion Danshen Duofensuanyan can effectively improve the blood rheology and the frequency of angina,and then improve heart functions.

Fas-associated death domain (FADD) is a signal connection protein in Fas/FasL apoptotic path which might play a key role on apoptosis by transferring apoptotic signal. To reveal the intracellular signal transduction molecules involved in the procedure of follicular development in bovine ovary, we cloned FADD gene in bovine ovary tissue with RT-PCR, deleted the termination codon in its cDNA and directionally cloned the amplified FADD gene into eukaryotic expression vector pAcGFP-N1 including AcGFP, successfully constructed the fusion protein recombinant plasmid. After identifying by restrictive enzyme Bgl II/EcoR I and sequencing, transfected pAcGFP-bFADD into CHO-K1 cell mediated by Lipofectamine 2000, observed the expression of AcGFP and detected the transcription and expression of FADD by RT-PCR and Western blotting. The results showed that the cattle FADD was successfully cloned, the pAcGFP-bFADD fusion protein recombinant plasmid was successfully constructed by introducing Bgl II, EcoR I cloning site at two ends of FADD open reading frame and inserting a Kozak sequence before start codon. AcGFP expression was detected as early as 24 h after transfection. The percentage of AcGFP positive cells reached about 65% after 24 h. A 654 bp transcription was amplified by RT-PCR, and 51.4 kD target protein was detected by Western blotting. Construction of pAcGFP-bFADD recombinant plasmid should be helpful for further understanding the mechanism of regulation of FADD on bovine oocytes formation and development.
Animals ; Base Sequence ; CHO Cells ; Cattle ; Cloning, Molecular ; Cricetinae ; Cricetulus ; DNA, Complementary ; genetics ; Fas-Associated Death Domain Protein ; biosynthesis ; genetics ; Female ; Genetic Vectors ; genetics ; Green Fluorescent Proteins ; biosynthesis ; genetics ; Molecular Sequence Data ; Oocytes ; cytology ; Open Reading Frames ; Ovary ; metabolism ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; Transfection

Objective:To investigate whether the image quality of total-body PET/CT (TB PET/CT) with 1 min acquisition can meet the clinical diagnostic requirements.Methods:From May 2019 to September 2021, a total of 90 malignant tumor patients (60 males, 30 females, age 31-86 years) with primary lesions confirmed by pathological diagnosis in Zhongshan Hospital, Fudan University were respectively analyzed. All patients underwent conventional PET/CT (C PET/CT) scan with conventional clinical acquisition and TB PET/CT scan with 1 min acquisition after injecting 18F-FDG in random order. Paired t test or Wilcoxon signed rank test was used to analyze the image quality of these two scans. Results:SUV max of primary lesions in TB PET/CT group was significantly higher than that in C PET/CT group (15.9(7.9, 24.6) vs 12.5(5.8, 16.6); z=8.14, P<0.001), so were signal-to-noise ratio (SNR) of the blood pool, liver, muscles (9.3±3.0, 11.4(9.5, 14.2), 8.3(7.3, 10.1) vs 6.2±1.7, 9.4(7.7, 11.8), 6.0(4.9, 7.1)), tumor-to-blood pool ratio (TBR) (9.3(4.3, 14.8) vs 8.5(4.3, 11.1)), tumor-to-liver ratio (TLR) (6.7(3.0, 10.4) vs 6.1(2.9, 7.7)), tumor-to-muscle ratio (TMR) (23.2(11.5, 38.0) vs 18.3(9.6, 26.6); t=9.36, z values: 4.44-7.40, all P<0.001). Conclusion:The image quality of TB PET/CT scan with 1 min acquisition can meet the diagnostic requirements, and is better than the C PET/CT image quality with conventional clinical acquisition.