Objective To study the current status of catheter - related thrombosis(CRT)in Chinese children through a retrospective analysis of the inpatients in the Department of Medicine,Beijing Children's Hospital Affiliated to Capital Medical University. Methods The clinical data of the inpatients with CRT from November 2010 to November 2013 were collected retrospectively,and the causes,clinical symptoms,diagnosis,treatment and prognosis were ana-lyzed. Results There were 42 cases of children with CRT in Beijing Children's Hospital Affiliated to Capital Medical University. Among the cases,the male to female ratio was 1. 0:0. 5;the median age of onset was 88(2 - 186)months with ﹤ 1 year old counted for 16. 7%(7 / 42 cases)and 13 - 14 years old counted for 11. 9%(5 / 42 cases);the distri-bution differences between the male and the female age were not significant(P = 0. 826). The median time from cathe-terization to CRT onset was 9(1 - 81)days,0 - 10 days after catheterization was the peak of onset(52. 5% ,21 / 40 ca-ses)followed by 10 - 20 days(35. 0% ,14 / 40 cases). The protopathy was usually hematologic tumor,kidney disease or deep fungal infection. Slightly more cases developed CRT on the right side(57. 1% ,24 / 42 cases)than on the left side (38. 1% ,18 / 42 cases). All cases were diagnosed by using B - ultrasound,of whom 28. 6%(12 / 42 cases)were symp-tom - free. After being diagnosed,7. 1%(3 / 42 cases)were treated with conservative methods such as immobilization of the affected limbs and hot compress;7. 1%(3 / 42 cases)had catheter removed;anticoagulant and/ or thrombolytics after catheter removal used in 33. 3% patients(14 / 42 cases). After 1 week,22 cases were reviewed,of whom 54. 5%(12 / 22 cases)had thrombosis reduced(all with intervention),thrombosis growing in 22. 7% patients(5 / 22 cases), and thrombosis did not change in 22. 7% patients(5 / 22 cases). Three cases needed re - catheterization after catheter removal,and all of 3 cases had CRT recurrences(100% ). Conclusions CRT is more common among infants and senior children. CRT usually develops within 20 days after catheterization. Children with hematologic tumor,kidney disease or deep fungal infection are more likely to have CRT. Routine ultrasound test should be conducted to monitor CRT in catheterized children. Once CRT is diagnosed,patients need to be treated with anticoagulants and/ or thrombo-lytics. Catheter should also be removed if necessary. Recatheterization can result in CRT recurrence.

Objective To study the lignans in Patrinia scabra. Methods The constituents were separated and purified by column chromatographies with silica gel, RP-silica gel, and Sephadex LH-20. Their structures were elucidated on the basis of spectral data (IR, MS, 1H-NMR , 13 C-NMR , DEPT, HMQC and HMBC). Results There were four lignans obtained from P. scabra with the structures identified as pinoresinol-4, 4′-di-O-?-D-glucopyranoside (Ⅰ), and matairesinol-4, 4′-di-O-?-D-glucopyranoside (Ⅱ), lariciresinol-4′-O-?-D-glucopyranoside (Ⅲ), and lariciresinol-4-O-?-D-glucopyranoside (Ⅳ). Conclusion All the four compounds are found in P. scabra for the first time. The NMR data of compound Ⅱ are given first.

Objective To investigate the impact of auto and allogenic mesenchymal stem cells (MSC) transplantation on hematopoietic reconstitution. Methods MSC from auto, donor bone marrow or embryonic tissue were cultured and expanded in vitro in the serum culture system. Five patients received hematopoietic stem cell transplantation (HSCT) were investigated. Case 1 of systemic lupus erythematosus and Case 2 of non-hodgkin' s lymphoma (NHL) received auto MSC transplant before auto-HSCT. Case 3 of paroxysmal nocturnal hemoglobinuria received HLA-identical allogenic MSC transplant before HLA-identical allo-HSCT.Case 4 of chronic myelocytic leukemia and Case 5 of NHL had delayed hematopoietic reconstitution (129th and 78th day, respectively) after allo- and auto-HSCT, respectively, and received MSC from embryonic tissue.Results Case 1, 2 and 3 had no manifested side effects after MSC transplantation combined with HSCT.Neutrophil count of case 1, 2, and 3 were over 0.5 ×109/L at 1st, 10th and 10th day, respectively, platelet count were over 20 ×109/L at 1st, 8th and 33th day, respectively, and agranulocytosis at Ost, 7th and 12th day, respectively. The treatment of embryonic tissue MSC transplant was confirmed to fail for Case 4 and 5.Conclusion The time of MSC transplant has a great impact on hematopoietic reconstitution. MSC transplantation and HSCT performed simultaneously can improve hematopoietic reconstitution. However, the impact of MSC on patients with delayed hematopoietic reconstitution after HSCT needs further study.

This review made a brief summary of several pharmacokinetic methods of traditional Chinese medicine (TCM ) prescription including the methods of biological effects ,plasma concentration ,integrated Pharmacokinetics of multiple effective components of TCM prescription and integrated pharmacokinetic‐pharmacodynamic of TCM prescription .The corresponding characteristics ,advantages and disadvantages of each of these methods were analyzed .And the progresses of the pharmacokinetic methods of TCM prescription were concluded and prospected .

Objective To screen and identify the main pro-angiogenic compounds of Shexiang Baoxin pill ( SBP) presenting in the plasma.Methods The pro-angiogenic effects of SBP and its compounds absorbed into blood were measured by the cell prolif-eration and cell migration assays by xCELLigence .And the cell tube formation and rat aortic ring models were established to evaluate their pro-angiogenic effect.Results SBP(10 -4 ~10 -2 μg/ml), ginsenoside Rg3(1 ~10 μmol/L) and ginsenoside Rh2(1 ~10μmol/L)significantly stimulated human umbilical vein endothelial cells (HUVECs) proliferation, migration and tube-like structures formation at different concentrations (P<0.05).In addition, compared to the control group, only the high concentration group of SBP (10 -2 μg/ml), Rg3(10 μmol/L) and Rh2(10 μmol/L)could induce endothelial cell sprouting from the aortic ring(P<0.05) .Conclusion SBP, ginsenosideRg3 and Rh2 exhibited significantly pro-angiogenic effect in vitro.

OBJECTIVETo investigate the chemical constituents from the whole plants of Senecio chrysanthemoides.

METHODConstituents were isolated by using a combination of various chromatographic techniques including column chromatography over silica gel, Sephadex LH-20, and ODS C18, as well as reversed-phase HPLC. Structures of the isolates were identified by spectroscopic and chemical methods.

RESULTEighteen glycosides were obtained from a H2O-soluble portion of an ethanolic extract of the whole plants of Senecio chrysanthemoides and their structures were elucidated as 5'-methoxyligusinenoside B (1), hyuganoside III b (2), citrusin A (3), alaschanioside A (4), citrusin B (5), dehydrodieoniferyl alcohol 4, gamma'-di-O-beta-D-glucopyranoside (6), osmanthuside G (7), syringin (8), dehydrosyringin (9), 2-(4-hydroxy-3,5-dimethoxyphenyl) ethanol 4-O-beta-D-glucopyranoside (10), 2-phenylethyl beta-gentiobioside (11), phenethyl beta-D-glucopyranoside (12), nikoenoside (13), benzyl beta-D-glucopyranoyl (1 --> 6 ) -beta-D-glucopyranoside (14), 3,5-dimethoxy-4-hydroxybenzyl alcohol 4-O-beta-D-glucopyranoside (15), icariside B2 (16), sonchuionoside C (17), and 1-[(beta-D-glucopyranosyloxy) methyl] -5,6-dihydropyrrolizin-7-one (18).

CONCLUSIONCompound 1 was a new lignan glycoside, and the remaining compounds were obtained from this plant for the first time.

Chromatography ; methods ; Glycosides ; chemistry ; isolation & purification ; Lignans ; chemistry ; isolation & purification ; Plant Extracts ; chemistry ; Plants, Medicinal ; chemistry ; Senecio ; chemistry

OBJECTIVETo investigate the chemical constituents from the whole plants of Senecio chrysanthemoides.

METHODThe chemical constituents were isolated and purified by chromatographic techniques over silica gel, Sephadex LH-20, preparative TLC and preparative HPLC. Structures of the compounds were identified by NMR and MS spectroscopic methods.

RESULTTwenty five compounds were obtained and their structures were elucidated as seneciphyline (1), senecionine (2) , 1,2-dihydrocacalohastine (3) , eu-desm-4( 15)-ene-1beta,6alpha-diol (4), 7,11,15-trimethyl- 3methylidenehexadecane-1,2-diol (5), faradiol 3-O-palmitate (6),maniladiol 3-O-palmitate (7), faradiol (8), maniladiol (9), beta-amyrin (10), alpha-amyrin (11), betulin (12), loranthol (13), (+)-syringaresinol (14) , 1-hydroxy-4-oxo-cyclohexane-1-acetate (15), 2, 6-dimethoxy-p-benzoquinone (16), stigmasta-5, 22-dien-3beta-hydroxy-7-one (17) , stigmasta-5, 22-dien-7-one (18) , stigmasta-4-en-3-one (19), stigmasta-4,22-dien-3-one (20), beta-sitosterol (21), stigmasterol (22), daucosterol (23), glycerol 1-hendecanoate (24), and methyl hendecanoate (25).

CONCLUSIONCompounds 5-9,13, 17-20 and 24 were obtained from the genus Senecio for the first time.

Acetates ; chemistry ; Chemical Fractionation ; Plant Extracts ; analysis ; isolation & purification ; Senecio ; chemistry

Objective To build hypoxia/reoxygenation injury model in cultured neonatal rat cardiomyocyte and screen active components from Shexiang Baoxin Pill ( SBP) absorbed in blood against hypoxia/reoxygenation injury .Methods Cardiomyocytes were isolated and purified from hearts of neonatal Sprague Dawley rats (1~3 days old) and were used to build hypoxia/reoxygenation injury model.The components of SBP absorbed in blood were screened by methyl thiazolil tetracolium (MTT) colorimetic method.Results SBP showed significant protective effect against cardiomyocytes hypoxia /reoxygenation injury atthe concentration of 50 μg/ml.Ginsen-oside Rb1, Rb2, bufalin and muscone of twenty components from SBP absorbed in blood also possessed significant protective effect a -gainst cardiomyocytes hypoxia/reoxygenation injury .Conclusion SBP have the protective activity against cardiomyocytes hypoxia /reoxygenation injury , and ginsenoside Rb1, Rb2, bufalin, muscone are the main active components of SBP .This experiment offered basis for further pharmacodynamics and mechanism study of SBP .