1. Clinical value of a rapid respiratory syncytial virus antigen detection in point-of-care testing
Yaxin DING ; Run TIAN ; Yuan QIAN ; Yu SUN ; Jie DENG ; Fang WANG ; Runan ZHU ; Linqing ZHAO
Chinese Journal of Pediatrics 2017;55(2):139-143
Objective:
To evaluate the clinical value of a rapid respiratory syncytial virus (RSV) antigen detection in point-of-care testing (POCT).
Method:
A total of 209 specimens, including 78 throat swabs (TS) and 131 nasopharyngeal aspirates (NPAs), were collected from inpatients who visited the Children′s Hospital Affiliated to the Capital Institute of Pediatrics and were diagnosed as acute respiratory infection from 5 January to 7 February, 2015. These specimens were tested for RSV by a rapid antigen detection kit which was compared with reverse transcription polymerase chain reaction (RT-PCR) and direct immunofluorescence assay (DFA) for RSV detection.
Result:
Compared with DFA for NPAs, the sensitivity and specificity of rapid antigen detection were 83.9% and 97.3%, respectively, with
2. Molecular biological and clinical characteristics of respiratory syncytial virus in children with bronchiolitis
Tuohui ZHANG ; Jie DENG ; Yuan QIAN ; Runan ZHU ; Yu SUN ; Fang WANG ; Run TIAN ; Linqing ZHAO
Chinese Journal of Pediatrics 2017;55(8):586-592
Objective:
To investigate the clinical characteristics of respiratory syncytial virus(RSV)bronchiolitis and molecular biological characteristics of RSV in children in Beijing.
Method:
In a systematic retrospective study, 2 296 nasopharyngeal aspirates (NPA) were collected from children diagnosed with bronchiolitis from July 2006 to June 2016 for respiratory virus screening using direct immunofluorescence assay (DFA). For specimens positive for RSV, subgroup A or B was confirmed by real time RT-PCR and genotype of RSV was determined by amplifying the full G glycoprotein gene and sequencing. Clinical data were evaluated by the modified Tal score to compare the severity between RSV subtypes, as well as genotypes. Statistical analyses were performed using
3. Epidemiological features of prevalent influenza A viruses in children with influenza-like illness during the 2004-2017 season in Beijing
Fang WANG ; Yuan QIAN ; Jie DENG ; Yu SUN ; Linqing ZHAO ; Run TIAN ; Runan ZHU
Chinese Journal of Pediatrics 2018;56(6):429-434
Objective:
To analyze and compare the epidemiological features of prevalent influenza A viruses in children in Beijing during 13 consecutive surveillance seasons from 2004 to 2017.
Methods:
This was a repeated cross section study. Throat swabs were collected weekly from children with influenza-like illnesses (ILI) who presented to the outpatient/emergency department of Children's Hospital, Capital Institute of Pediatrics during the period from September, 2004 to August, 2017. All of the specimens were inoculated into Madin Darby canine kidney (MDCK) cells to isolate influenza viruses followed by identifying different types of influenza viruses with reference antisera by hemagglutination-inhibition assay. Descriptive statistics,
4.Phenylpropanoid pathway in plants and its role in response to heavy metal stress: a review.
Wenjia GE ; Jianpan XIN ; Runan TIAN
Chinese Journal of Biotechnology 2023;39(2):425-445
Phenylpropanoid metabolic pathway is one of the most important secondary metabolic pathways in plants. It directly or indirectly plays an antioxidant role in plant resistance to heavy metal stress, and can improve the absorption and stress tolerance of plants to heavy metal ions. In this paper, the core reactions and key enzymes of the phenylpropanoid metabolic pathway were summarized, and the biosynthetic processes of key metabolites such as lignin, flavonoids and proanthocyanidins and relevant mechanisms were analyzed. Based on this, the mechanisms of key products of phenylpropanoid metabolic pathway in response to heavy metal stress were discussed. The perspectives on the involvement of phenylpropanoid metabolism in plant defense against heavy metal stress provides a theoretical basis for improving the phytoremediation efficiency of heavy metal polluted environment.
Plants/metabolism*
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Metals, Heavy/metabolism*
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Flavonoids/metabolism*
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Biodegradation, Environmental
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Antioxidants