Self-cloning is a favorable technique in modification of industrial microorganisms especially food microorganisms because of its bio-safety. A survey and outlook of research in breeding of self-cloning industrial brewing yeast were introduced in this study.

The yeast fusant ZFF-28, which is high in biomass production and rich in selenium, was constructed after mutagenesis and protoplasts fusion between yeast strains. The total selenium content of ZFF-28 is 1.8 and 1.0 times higher than that of the parental strains Saccharomyces cerevisiae ZY-67 and Saccharomyces kluyveri SZY-198 respectively. Using single factor tests and a L16(4(3) x 2(1)) orthogonal design, the cultivation conditions was optimized as: 50mL culture in 250mL shake flasks in molasses containing 6% sugar and 60microg/mL Se at 28 degree C for 25h at 220 r/min, with the initial pH adjusted to 6.0 - 6.5. Under the optimized conditions, the biomass (dry weight) reached 8.2g/L and the Se content of the cells reached 2050microg/g, with organic and inorganic Se contents being 91% and 9% respectively.
Biomass ; Hydrogen-Ion Concentration ; Saccharomyces ; genetics ; growth & development ; metabolism ; Saccharomyces cerevisiae ; genetics ; metabolism ; Selenium ; metabolism ; Selenium Compounds ; metabolism

Aged ; Aged, 80 and over ; Foreign-Body Migration ; etiology ; Hernia, Inguinal ; surgery ; Humans ; Intestinal Obstruction ; etiology ; Male ; Postoperative Complications ; etiology ; Surgical Mesh

Adult ; Gallbladder Diseases ; pathology ; Gastric Mucosa ; pathology ; Humans ; Male

OBJECTIVETo discuss the safety and the feasibility of laparoscopic selective portal inflow occlusion.

METHODSFrom January 2005 to February 2006, 7 patients were performed by laparoscopic hepatectomy with selective portal inflow occlusion. The patients included 1 male and 6 females, the mean age was 48.9 years, ranged from 31 to 70 years. Procedure included left lateral segmentectomies (4 patients), left hemihepatectomies (2 patients), and right hemihepatectomies (1 patient). Portal vein, hepatic artery and their branches were exposed by blunt dissection and performed selective portal occlusion with absorbable clips.

RESULTSThere is no conversion, no mortality, and no postoperative complication. Seven selective portal inflow occlusions were performed successfully. The mean operating time was 188.7 min (range 70 min to 300 min), the mean intraoperative blood loss was 625 ml (range 350 ml to 1000 ml), and the mean postoperative hospital stay was 8.4 days.

CONCLUSIONSLaparoscopic selective portal inflow occlusion is a safe and feasible technique.

Adult ; Aged ; Female ; Follow-Up Studies ; Hemostasis, Surgical ; methods ; Hepatectomy ; methods ; Humans ; Laparoscopy ; Male ; Middle Aged

Objective To investigate the effect of RablA on proliferation and apoptosis of multiple myeloma (MM) cell line 8226. Methods The siRNA interference was used to knockdown RablA gene. The multiple myeloma cell line 8226 was divided into blank control group, negative control group and RablA siRNA group. In the blank control group, the multiple myeloma cells were not treated. Multiple myeloma cells 8226 in the negative control group were transfected with negative control siRNA. The RablA siRNA group was transfected with Rabl A-targeted siRNA. The effect of RablA on multiple myeloma cell 8226 proliferation was analyzed by colony forming test and cell counting kit-8 (CCK-8) assay. The apoptosis of multiple myeloma cell 8226 was detected by flow cytometry. Western blotting and Real-time PCR were used to observe the effect of RablA siRNA on the expression of c-Myc, cyclin D1, Bcl-2 and Bax. Results The expressions of RablA mRNA and RablA protein in the RablA siRNA group were significantly down-regulated compared with those in the negative control group. The result of colony formation and CCK-8 assay showed that RablA siRNA inhibit the proliferation of multiple myeloma cells 8226. The early and late apoptosis ratio of multiple myeloma cell 8226 in RablA siRNA group increased significantly compared with the negative control group (P<0.05). The expression of cyclin D1 and Bcl-2 in the RablA siRNA group were significantly down-regulated compared with the negative control group (P<0.05), and the expression of c-Myc and Bax were significantly up-regulated compared with the negative control group (P<0.05). Conclusion RablA may promote the proliferation of multiple myeloma cells 8226 by regulating the expression of c-Myc, cyclin Dl, Bcl-2 and Bax, while RablA siRNA can effectively inhibit the expression of RablA in rpmi-8226 cells, thereby inhibiting its proliferation and promoting apoptosis.

Adult ; Hemostasis, Surgical ; methods ; Hepatectomy ; methods ; Humans ; Laparoscopy ; methods ; Liver ; blood supply ; Middle Aged ; Treatment Outcome

Adult ; Cysts ; pathology ; surgery ; Endoscopes ; Female ; Humans ; Liver Diseases ; pathology ; surgery ; Surgical Procedures, Operative ; methods ; Tomography, X-Ray Computed ; Treatment Outcome

Adult ; Aged ; Female ; Humans ; Intestine, Small ; surgery ; Lymphangiectasis, Intestinal ; pathology ; surgery ; Male ; Middle Aged

Female ; Hepatectomy ; methods ; Humans ; Laparoscopy ; methods ; Liver Neoplasms ; surgery ; Middle Aged